Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production

Abstract The biofuels are receiving considerable attention as a substitute for petro diesel. For microalgae, the cell density or biomass and lipid contents are key components for biodiesel production. This study was conducted to develop favorable culture conditions for Dunaliella salina to maximize...

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Main Authors: Rajper Aftab Ahmed, Meilin He, Rajper Asma Aftab, Shiyan Zheng, Mostafa Nagi, Ramadan Bakri, Changhai Wang
Format: Article
Language:English
Published: Nature Portfolio 2017-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-07540-x
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author Rajper Aftab Ahmed
Meilin He
Rajper Asma Aftab
Shiyan Zheng
Mostafa Nagi
Ramadan Bakri
Changhai Wang
author_facet Rajper Aftab Ahmed
Meilin He
Rajper Asma Aftab
Shiyan Zheng
Mostafa Nagi
Ramadan Bakri
Changhai Wang
author_sort Rajper Aftab Ahmed
collection DOAJ
description Abstract The biofuels are receiving considerable attention as a substitute for petro diesel. For microalgae, the cell density or biomass and lipid contents are key components for biodiesel production. This study was conducted to develop favorable culture conditions for Dunaliella salina to maximize its biomass and lipid accumulation. The effect of salinity (0.5 to 2.5 M NaCl) on the cell population, biochemical composition, and lipid output of Dunaliella salina was examined under a controlled environment for 21 days. Maximum growth (6.57 × 107 to 7.17 × 107cells mL−1) potentials were observed at 1.5 to 2 M NaCl. The photosynthetic pigments and carbohydrates also showed trends similar to growth. The maximum carotenoid level (5.16 mg L−1) was recorded at 2 M NaCl. Almost all physicochemical parameters increased with increases in salinity, biomass (1231.66 ± 1.26 mg L−1) and lipid content (248.33 mg L−1), as recorded at 2 M NaCl. Based on fluorescence intensity, the highest values (11.84 × 107cells/ml) of neutral lipids and total lipids (22.28%) were recorded at optimum salinity levels. The present study suggests that a high biomass and lipid accumulation of Dunaliella salina SA 134 could be obtained at the 2 M NaCl level.
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spelling doaj.art-ce275543869441ec89f83803f19e3ca42022-12-21T19:10:51ZengNature PortfolioScientific Reports2045-23222017-08-017111010.1038/s41598-017-07540-xBioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid productionRajper Aftab Ahmed0Meilin He1Rajper Asma Aftab2Shiyan Zheng3Mostafa Nagi4Ramadan Bakri5Changhai Wang6Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityJiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityJiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityJiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityJiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityJiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityJiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural UniversityAbstract The biofuels are receiving considerable attention as a substitute for petro diesel. For microalgae, the cell density or biomass and lipid contents are key components for biodiesel production. This study was conducted to develop favorable culture conditions for Dunaliella salina to maximize its biomass and lipid accumulation. The effect of salinity (0.5 to 2.5 M NaCl) on the cell population, biochemical composition, and lipid output of Dunaliella salina was examined under a controlled environment for 21 days. Maximum growth (6.57 × 107 to 7.17 × 107cells mL−1) potentials were observed at 1.5 to 2 M NaCl. The photosynthetic pigments and carbohydrates also showed trends similar to growth. The maximum carotenoid level (5.16 mg L−1) was recorded at 2 M NaCl. Almost all physicochemical parameters increased with increases in salinity, biomass (1231.66 ± 1.26 mg L−1) and lipid content (248.33 mg L−1), as recorded at 2 M NaCl. Based on fluorescence intensity, the highest values (11.84 × 107cells/ml) of neutral lipids and total lipids (22.28%) were recorded at optimum salinity levels. The present study suggests that a high biomass and lipid accumulation of Dunaliella salina SA 134 could be obtained at the 2 M NaCl level.https://doi.org/10.1038/s41598-017-07540-x
spellingShingle Rajper Aftab Ahmed
Meilin He
Rajper Asma Aftab
Shiyan Zheng
Mostafa Nagi
Ramadan Bakri
Changhai Wang
Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production
Scientific Reports
title Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production
title_full Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production
title_fullStr Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production
title_full_unstemmed Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production
title_short Bioenergy application of Dunaliella salina SA 134 grown at various salinity levels for lipid production
title_sort bioenergy application of dunaliella salina sa 134 grown at various salinity levels for lipid production
url https://doi.org/10.1038/s41598-017-07540-x
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