Efficient regeneration system applicable to five Musa cultivars
Banana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Hybridization is seriously hampered by the long generation time, polyploidy, and sterility of most cultivars. Establishment of an efficient regeneration and transformation system for banana is critical fo...
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| Format: | Article |
| Language: | English |
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Higher Education Press
2016-12-01
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| Series: | Frontiers of Agricultural Science and Engineering |
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| Online Access: | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1481096098202-1400245432.pdf |
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| author | Juhua LIU,Peiguang SUN,Jing ZHANG,Jiashui WANG,Jianbin ZHANG,Jingyi WANG,Caihong JIA,Pengzhao GAO,Biyu XU,Zhiqiang JIN |
| author_facet | Juhua LIU,Peiguang SUN,Jing ZHANG,Jiashui WANG,Jianbin ZHANG,Jingyi WANG,Caihong JIA,Pengzhao GAO,Biyu XU,Zhiqiang JIN |
| author_sort | Juhua LIU,Peiguang SUN,Jing ZHANG,Jiashui WANG,Jianbin ZHANG,Jingyi WANG,Caihong JIA,Pengzhao GAO,Biyu XU,Zhiqiang JIN |
| collection | DOAJ |
| description | Banana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Hybridization is seriously hampered by the long generation time, polyploidy, and sterility of most cultivars. Establishment of an efficient regeneration and transformation system for banana is critical for their genetic improvement. An efficient and reproducible transformation system for banana using direct organogenesis was developed. Media containing benzylaminopurine (BA) combined with one of four other growth regulators was evaluated for the regeneration efficiency of five Musa cultivars and the ability to induce/support development of new banana shoots. The result indicated that the greatest number of shoots per explant for all five Musa cultivars was obtained using MS medium supplemented with 8.9 mmol·L<sup>−1</sup> BA and 9.1 mmol·L<sup>−1</sup> thidiazuron (TDZ). In 240–270 d, one immature male flower could regenerate between 380 and 456, 310–372, 200–240, 130–156, and 100–130 well-developed shoots for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Such a system will facilitate molecular breeding and functional genomics of banana. |
| first_indexed | 2024-12-13T13:57:24Z |
| format | Article |
| id | doaj.art-ce28307df1ce4a8a99dc21d91cd56f03 |
| institution | Directory Open Access Journal |
| issn | 2095-7505 |
| language | English |
| last_indexed | 2024-12-13T13:57:24Z |
| publishDate | 2016-12-01 |
| publisher | Higher Education Press |
| record_format | Article |
| series | Frontiers of Agricultural Science and Engineering |
| spelling | doaj.art-ce28307df1ce4a8a99dc21d91cd56f032022-12-21T23:42:51ZengHigher Education PressFrontiers of Agricultural Science and Engineering2095-75052016-12-013433033410.15302/J-FASE-2016118Efficient regeneration system applicable to five Musa cultivarsJuhua LIU,Peiguang SUN,Jing ZHANG,Jiashui WANG,Jianbin ZHANG,Jingyi WANG,Caihong JIA,Pengzhao GAO,Biyu XU,Zhiqiang JIN01. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, ChinaBanana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Hybridization is seriously hampered by the long generation time, polyploidy, and sterility of most cultivars. Establishment of an efficient regeneration and transformation system for banana is critical for their genetic improvement. An efficient and reproducible transformation system for banana using direct organogenesis was developed. Media containing benzylaminopurine (BA) combined with one of four other growth regulators was evaluated for the regeneration efficiency of five Musa cultivars and the ability to induce/support development of new banana shoots. The result indicated that the greatest number of shoots per explant for all five Musa cultivars was obtained using MS medium supplemented with 8.9 mmol·L<sup>−1</sup> BA and 9.1 mmol·L<sup>−1</sup> thidiazuron (TDZ). In 240–270 d, one immature male flower could regenerate between 380 and 456, 310–372, 200–240, 130–156, and 100–130 well-developed shoots for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Such a system will facilitate molecular breeding and functional genomics of banana.http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1481096098202-1400245432.pdfbanana (Musa spp)|system|regeneration |
| spellingShingle | Juhua LIU,Peiguang SUN,Jing ZHANG,Jiashui WANG,Jianbin ZHANG,Jingyi WANG,Caihong JIA,Pengzhao GAO,Biyu XU,Zhiqiang JIN Efficient regeneration system applicable to five Musa cultivars Frontiers of Agricultural Science and Engineering banana (Musa spp)|system|regeneration |
| title | Efficient regeneration system applicable to five Musa cultivars |
| title_full | Efficient regeneration system applicable to five Musa cultivars |
| title_fullStr | Efficient regeneration system applicable to five Musa cultivars |
| title_full_unstemmed | Efficient regeneration system applicable to five Musa cultivars |
| title_short | Efficient regeneration system applicable to five Musa cultivars |
| title_sort | efficient regeneration system applicable to five musa cultivars |
| topic | banana (Musa spp)|system|regeneration |
| url | http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/1481096098202-1400245432.pdf |
| work_keys_str_mv | AT juhualiupeiguangsunjingzhangjiashuiwangjianbinzhangjingyiwangcaihongjiapengzhaogaobiyuxuzhiqiangjin efficientregenerationsystemapplicabletofivemusacultivars |