Characterisation of Cooked Cheese Flavour: Non-Volatile Components

This work examined the role of selected non-volatile compounds in cooked cheese flavour, both as tastants and as precursors of aroma generation in the Maillard reaction. The effect of cooking on the concentration of selected non-volatile compounds (organic acids, sugars, amino acids, γ-glutamyl dipe...

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Main Authors: Rosa C. Sullivan, Samantha Nottage, Fiyinfolu Makinwa, Maria Jose Oruna-Concha, Colette C. Fagan, Jane K. Parker
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/12/20/3749
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author Rosa C. Sullivan
Samantha Nottage
Fiyinfolu Makinwa
Maria Jose Oruna-Concha
Colette C. Fagan
Jane K. Parker
author_facet Rosa C. Sullivan
Samantha Nottage
Fiyinfolu Makinwa
Maria Jose Oruna-Concha
Colette C. Fagan
Jane K. Parker
author_sort Rosa C. Sullivan
collection DOAJ
description This work examined the role of selected non-volatile compounds in cooked cheese flavour, both as tastants and as precursors of aroma generation in the Maillard reaction. The effect of cooking on the concentration of selected non-volatile compounds (organic acids, sugars, amino acids, γ-glutamyl dipeptides, and diketopiperazines) in six cheeses (mature Cheddar, mozzarella, Parmesan, and mild Cheddar (low, medium, and high fat)) was determined. Sugars, amino acids, and γ-glutamyl dipeptides were extracted and analysed by LC, whereas diketopiperazines were extracted by solid-phase extraction and analysed by GC-MS. Sugars, amino acids, and γ-glutamyl dipeptides decreased in concentration during cooking, whereas diketopiperazines and some organic acids increased in concentration. Diketopiperazines were above the taste threshold in some cooked cheeses and below the threshold in uncooked cheeses. The role of fat content in cooked cheese flavour is discussed. Furthermore, γ-glutamyl dipeptide concentration increased during 24 months of ageing in low, medium, and high-fat Cheddars, with similar levels of γ-glutamyl dipeptide detected in aged low and high-fat Cheddars. This work will give valuable insight for the dairy industry to inform the development of cheeses, especially low-fat variants, for use in cooked foods.
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spelling doaj.art-ce2aa821221241709a806d5fcb0f25bc2023-11-19T16:29:13ZengMDPI AGFoods2304-81582023-10-011220374910.3390/foods12203749Characterisation of Cooked Cheese Flavour: Non-Volatile ComponentsRosa C. Sullivan0Samantha Nottage1Fiyinfolu Makinwa2Maria Jose Oruna-Concha3Colette C. Fagan4Jane K. Parker5Department of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6DZ, UKDepartment of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6DZ, UKDepartment of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6DZ, UKDepartment of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6DZ, UKDepartment of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6DZ, UKDepartment of Food and Nutritional Sciences, University of Reading, Whiteknights, Reading RG6 6DZ, UKThis work examined the role of selected non-volatile compounds in cooked cheese flavour, both as tastants and as precursors of aroma generation in the Maillard reaction. The effect of cooking on the concentration of selected non-volatile compounds (organic acids, sugars, amino acids, γ-glutamyl dipeptides, and diketopiperazines) in six cheeses (mature Cheddar, mozzarella, Parmesan, and mild Cheddar (low, medium, and high fat)) was determined. Sugars, amino acids, and γ-glutamyl dipeptides were extracted and analysed by LC, whereas diketopiperazines were extracted by solid-phase extraction and analysed by GC-MS. Sugars, amino acids, and γ-glutamyl dipeptides decreased in concentration during cooking, whereas diketopiperazines and some organic acids increased in concentration. Diketopiperazines were above the taste threshold in some cooked cheeses and below the threshold in uncooked cheeses. The role of fat content in cooked cheese flavour is discussed. Furthermore, γ-glutamyl dipeptide concentration increased during 24 months of ageing in low, medium, and high-fat Cheddars, with similar levels of γ-glutamyl dipeptide detected in aged low and high-fat Cheddars. This work will give valuable insight for the dairy industry to inform the development of cheeses, especially low-fat variants, for use in cooked foods.https://www.mdpi.com/2304-8158/12/20/3749cooked cheesetastantdiketopiperazineγ-glutamyl-dipeptidecheddarmozzarella
spellingShingle Rosa C. Sullivan
Samantha Nottage
Fiyinfolu Makinwa
Maria Jose Oruna-Concha
Colette C. Fagan
Jane K. Parker
Characterisation of Cooked Cheese Flavour: Non-Volatile Components
Foods
cooked cheese
tastant
diketopiperazine
γ-glutamyl-dipeptide
cheddar
mozzarella
title Characterisation of Cooked Cheese Flavour: Non-Volatile Components
title_full Characterisation of Cooked Cheese Flavour: Non-Volatile Components
title_fullStr Characterisation of Cooked Cheese Flavour: Non-Volatile Components
title_full_unstemmed Characterisation of Cooked Cheese Flavour: Non-Volatile Components
title_short Characterisation of Cooked Cheese Flavour: Non-Volatile Components
title_sort characterisation of cooked cheese flavour non volatile components
topic cooked cheese
tastant
diketopiperazine
γ-glutamyl-dipeptide
cheddar
mozzarella
url https://www.mdpi.com/2304-8158/12/20/3749
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AT mariajoseorunaconcha characterisationofcookedcheeseflavournonvolatilecomponents
AT colettecfagan characterisationofcookedcheeseflavournonvolatilecomponents
AT janekparker characterisationofcookedcheeseflavournonvolatilecomponents