Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans
Objective(s):Sulfur mustard (SM) was used by the Iraqi army against the Iranian troops in the Iran-Iraq war from 1983–1988. This chemical gas affects different organs including the skin, lungs and the hematopoietic system. Any exposure to SM increases the risk of chromosomal breaking, hyperdiploidy...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2015-09-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_5205_d5c445acb41970e84d5705443872b315.html |
| _version_ | 1823998739049611264 |
|---|---|
| author | Hossein Ayatollahi Mohammad Rafiee Mohammad-Reza Keramati Mahdi Balali-Mood Ali Asgharzadeh Mohammad Hadi Sadeghian Maryam Sheikhi Nafiseh Amini Azam Moradi Zarmehri |
| author_facet | Hossein Ayatollahi Mohammad Rafiee Mohammad-Reza Keramati Mahdi Balali-Mood Ali Asgharzadeh Mohammad Hadi Sadeghian Maryam Sheikhi Nafiseh Amini Azam Moradi Zarmehri |
| author_sort | Hossein Ayatollahi |
| collection | DOAJ |
| description | Objective(s):Sulfur mustard (SM) was used by the Iraqi army against the Iranian troops in the Iran-Iraq war from 1983–1988. This chemical gas affects different organs including the skin, lungs and the hematopoietic system. Any exposure to SM increases the risk of chromosomal breaking, hyperdiploidy and hypodiploidy. Studies have shown that the risk for acute myeloblastic and lymphoblastic leukemia increases in veterans exposed to SM. FLT3 mutations including ITD and TKD mutations had been observed in some cases of leukemia. Therefore, we aimed to investigate the frequency of FLT3-TKD835 mutations in the veterans exposed to SM agent. Materials and Methods: We studied 42 patients who were exposed to SM during the war in Khorasan Razavi province, Mashhad, Iran in 2012. As control group, 30 healthy males were selected from first-degree relatives of the patients. For assessment of TKD835 mutation, DNA was extracted and RFLP-PCR was performed. Results: Analysis of RFLP-PCR data showed no FLT-3 TKD mutation in any of the patients. Conclusion: Although contact with SM can increase the risk of malignancy especially hematologic neoplasms, results of the study show that another mechanism of leukemogenesis, other than FLT3-TKD mutation, may be the reason for increased risk of leukemia in SM toxicity. |
| first_indexed | 2024-12-18T17:34:00Z |
| format | Article |
| id | doaj.art-ce2dc1002b634b7c9ac812f45c228128 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T17:34:00Z |
| publishDate | 2015-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ce2dc1002b634b7c9ac812f45c2281282022-12-21T20:59:24ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-09-011898568605205Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veteransHossein Ayatollahi0Mohammad Rafiee1Mohammad-Reza Keramati2Mahdi Balali-Mood3Ali Asgharzadeh4Mohammad Hadi Sadeghian5Maryam Sheikhi6Nafiseh Amini7Azam Moradi Zarmehri8Cancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranFaculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranMedical Toxicology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranFaculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranFaculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective(s):Sulfur mustard (SM) was used by the Iraqi army against the Iranian troops in the Iran-Iraq war from 1983–1988. This chemical gas affects different organs including the skin, lungs and the hematopoietic system. Any exposure to SM increases the risk of chromosomal breaking, hyperdiploidy and hypodiploidy. Studies have shown that the risk for acute myeloblastic and lymphoblastic leukemia increases in veterans exposed to SM. FLT3 mutations including ITD and TKD mutations had been observed in some cases of leukemia. Therefore, we aimed to investigate the frequency of FLT3-TKD835 mutations in the veterans exposed to SM agent. Materials and Methods: We studied 42 patients who were exposed to SM during the war in Khorasan Razavi province, Mashhad, Iran in 2012. As control group, 30 healthy males were selected from first-degree relatives of the patients. For assessment of TKD835 mutation, DNA was extracted and RFLP-PCR was performed. Results: Analysis of RFLP-PCR data showed no FLT-3 TKD mutation in any of the patients. Conclusion: Although contact with SM can increase the risk of malignancy especially hematologic neoplasms, results of the study show that another mechanism of leukemogenesis, other than FLT3-TKD mutation, may be the reason for increased risk of leukemia in SM toxicity.http://ijbms.mums.ac.ir/pdf_5205_d5c445acb41970e84d5705443872b315.htmlFLT3-TKD835 mutationIranLeukemiaSulfur mustard |
| spellingShingle | Hossein Ayatollahi Mohammad Rafiee Mohammad-Reza Keramati Mahdi Balali-Mood Ali Asgharzadeh Mohammad Hadi Sadeghian Maryam Sheikhi Nafiseh Amini Azam Moradi Zarmehri Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans Iranian Journal of Basic Medical Sciences FLT3-TKD835 mutation Iran Leukemia Sulfur mustard |
| title | Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans |
| title_full | Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans |
| title_fullStr | Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans |
| title_full_unstemmed | Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans |
| title_short | Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans |
| title_sort | lack of flt3 tkd835 gene mutation in toxicity of sulfur mustard in iranian veterans |
| topic | FLT3-TKD835 mutation Iran Leukemia Sulfur mustard |
| url | http://ijbms.mums.ac.ir/pdf_5205_d5c445acb41970e84d5705443872b315.html |
| work_keys_str_mv | AT hosseinayatollahi lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT mohammadrafiee lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT mohammadrezakeramati lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT mahdibalalimood lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT aliasgharzadeh lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT mohammadhadisadeghian lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT maryamsheikhi lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT nafisehamini lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans AT azammoradizarmehri lackofflt3tkd835genemutationintoxicityofsulfurmustardiniranianveterans |