Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population
Abstract Background Sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. It has the highest biomass production reaching 1.9 billion tonnes in 2014 worldwide. Results To i...
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| Format: | Article |
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BMC
2017-10-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-017-4158-8 |
| _version_ | 1819210862426062848 |
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| author | Ching Man Wai Jisen Zhang Tyler C. Jones Chifumi Nagai Ray Ming |
| author_facet | Ching Man Wai Jisen Zhang Tyler C. Jones Chifumi Nagai Ray Ming |
| author_sort | Ching Man Wai |
| collection | DOAJ |
| description | Abstract Background Sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. It has the highest biomass production reaching 1.9 billion tonnes in 2014 worldwide. Results To improve sugarcane biomass accumulation, we developed an interspecific cross between Saccharum officinarum ‘LA Purple’ and Saccharum robustum ‘MOL5829’. Selected F1 individuals were self-pollinated to generate a transgressive F2 population with a wide range of biomass yield. Leaf and stem internodes of fourteen high biomass and eight low biomass F2 extreme segregants were used for RNA-seq to decipher the molecular mechanism of rapid plant growth and dry weight accumulation. Gene Ontology terms involved in cell wall metabolism and carbohydrate catabolism were enriched among 3274 differentially expressed genes between high and low biomass groups. Up-regulation of cellulose metabolism, pectin degradation and lignin biosynthesis genes were observed in the high biomass group, in conjunction with higher transcript levels of callose metabolic genes and the cell wall loosening enzyme expansin. Furthermore, UDP-glucose biosynthesis and sucrose conversion genes were differentially expressed between the two groups. A positive correlation between stem glucose, but not sucrose, levels and dry weight was detected. Conclusions We thus postulated that the high biomass sugarcane plants rapidly convert sucrose to UDP-glucose, which is the building block of cell wall polymers and callose, in order to maintain the rapid plant growth. The gene interaction of cell wall metabolism, hexose allocation and cell division contributes to biomass yield. |
| first_indexed | 2024-12-23T06:17:55Z |
| format | Article |
| id | doaj.art-ce347062d2c043a08960e8c2cdcf40ac |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-23T06:17:55Z |
| publishDate | 2017-10-01 |
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| series | BMC Genomics |
| spelling | doaj.art-ce347062d2c043a08960e8c2cdcf40ac2022-12-21T17:57:15ZengBMCBMC Genomics1471-21642017-10-0118111410.1186/s12864-017-4158-8Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 populationChing Man Wai0Jisen Zhang1Tyler C. Jones2Chifumi Nagai3Ray Ming4FAFU and UIUC-SIB Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Corps, Ministry of Education, Fujian Agriculture and Forestry UniversityFAFU and UIUC-SIB Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Corps, Ministry of Education, Fujian Agriculture and Forestry UniversityHawaii Agriculture Research CenterHawaii Agriculture Research CenterFAFU and UIUC-SIB Joint Center for Genomics and Biotechnology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Key Laboratory of Genetics, Breeding and Multiple Utilization of Corps, Ministry of Education, Fujian Agriculture and Forestry UniversityAbstract Background Sugarcane is an emerging dual-purpose biofuel crop for energy and sugar production, owing to its rapid growth rate, high sucrose storage in the stems, and high lignocellulosic yield. It has the highest biomass production reaching 1.9 billion tonnes in 2014 worldwide. Results To improve sugarcane biomass accumulation, we developed an interspecific cross between Saccharum officinarum ‘LA Purple’ and Saccharum robustum ‘MOL5829’. Selected F1 individuals were self-pollinated to generate a transgressive F2 population with a wide range of biomass yield. Leaf and stem internodes of fourteen high biomass and eight low biomass F2 extreme segregants were used for RNA-seq to decipher the molecular mechanism of rapid plant growth and dry weight accumulation. Gene Ontology terms involved in cell wall metabolism and carbohydrate catabolism were enriched among 3274 differentially expressed genes between high and low biomass groups. Up-regulation of cellulose metabolism, pectin degradation and lignin biosynthesis genes were observed in the high biomass group, in conjunction with higher transcript levels of callose metabolic genes and the cell wall loosening enzyme expansin. Furthermore, UDP-glucose biosynthesis and sucrose conversion genes were differentially expressed between the two groups. A positive correlation between stem glucose, but not sucrose, levels and dry weight was detected. Conclusions We thus postulated that the high biomass sugarcane plants rapidly convert sucrose to UDP-glucose, which is the building block of cell wall polymers and callose, in order to maintain the rapid plant growth. The gene interaction of cell wall metabolism, hexose allocation and cell division contributes to biomass yield.http://link.springer.com/article/10.1186/s12864-017-4158-8SaccharumBiomassTranscriptomeCell wall metabolismHexose allocation |
| spellingShingle | Ching Man Wai Jisen Zhang Tyler C. Jones Chifumi Nagai Ray Ming Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population BMC Genomics Saccharum Biomass Transcriptome Cell wall metabolism Hexose allocation |
| title | Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population |
| title_full | Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population |
| title_fullStr | Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population |
| title_full_unstemmed | Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population |
| title_short | Cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a Saccharum interspecific F2 population |
| title_sort | cell wall metabolism and hexose allocation contribute to biomass accumulation in high yielding extreme segregants of a saccharum interspecific f2 population |
| topic | Saccharum Biomass Transcriptome Cell wall metabolism Hexose allocation |
| url | http://link.springer.com/article/10.1186/s12864-017-4158-8 |
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