Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated Membranes
Introduction: Chlorhexidine (CHX) has been used for some time in clinical practice as a local antiseptic agent with excellent efficacy. The combination of CHX with APRF (Advanced-platelet rich fibrin) membrane has the potential to stimulate tissue regeneration and to provide a bactericidal effect. W...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/4/226 |
| _version_ | 1797457023537774592 |
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| author | Tasho Gavrailov Ivan Chenchev Maria Gevezova Milena Draganova Victoria Sarafian |
| author_facet | Tasho Gavrailov Ivan Chenchev Maria Gevezova Milena Draganova Victoria Sarafian |
| author_sort | Tasho Gavrailov |
| collection | DOAJ |
| description | Introduction: Chlorhexidine (CHX) has been used for some time in clinical practice as a local antiseptic agent with excellent efficacy. The combination of CHX with APRF (Advanced-platelet rich fibrin) membrane has the potential to stimulate tissue regeneration and to provide a bactericidal effect. We hypothesize that this may reduce the rate of infections development and protect cell viability. Aim: The aim of this study was two-fold—to create a stable APRF membrane treated with different concentrations of CHX (0.01% and 0.02%) and to monitor its effect on the viability of PDL cells in vitro. This benefits the introduction of a new protocol for APRF membrane production -CHX-PRF and enriches the available evidence on the effect of this antiseptic agent on PDL (Periodontal ligament) cells. Materials and methods: APRF membranes were prepared by the addition of two concentrations (0.01% and 0.02%) of CHX. Membranes without the antiseptic were also prepared and used as control samples. PDL cells were cultivated on the membranes for 72 h. Cell number and vitality were examined by fluorescent cell viability assays. Results: Our results demonstrated that a concentration of 0.01% CHX allowed the production of a stable APRF membrane. This concentration slightly reduced the viability of PDL cells to 96.7%, but significantly decreased the average number of cells attached to the membrane—149 ± 16.5 cells/field compared to controls −336 ± 26.9 cells/field. APRF-CHX 0.02% membranes were unstable, indicating a dose-dependent cytotoxic effect of CHX. Conclusions: The introduced novel protocol leads to the production of a new type of APRF membrane—CHX-PRF. The incorporation of an antiseptic into the APRF membrane can improve its bactericidal activity and might serve as an important step for the prevention of postoperative infections. |
| first_indexed | 2024-03-09T16:16:12Z |
| format | Article |
| id | doaj.art-ce34c55aa1224878a8c7f5b6d5573368 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T16:16:12Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-ce34c55aa1224878a8c7f5b6d55733682023-11-24T15:49:49ZengMDPI AGJournal of Functional Biomaterials2079-49832022-11-0113422610.3390/jfb13040226Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated MembranesTasho Gavrailov0Ivan Chenchev1Maria Gevezova2Milena Draganova3Victoria Sarafian4Department of Oral Surgery, Medical University—Plovdiv, 4000 Plovdiv, BulgariaDepartment of Oral Surgery, Medical University—Plovdiv, 4000 Plovdiv, BulgariaResearch Institute at MU-Plovdiv, 4000 Plovdiv, BulgariaResearch Institute at MU-Plovdiv, 4000 Plovdiv, BulgariaResearch Institute at MU-Plovdiv, 4000 Plovdiv, BulgariaIntroduction: Chlorhexidine (CHX) has been used for some time in clinical practice as a local antiseptic agent with excellent efficacy. The combination of CHX with APRF (Advanced-platelet rich fibrin) membrane has the potential to stimulate tissue regeneration and to provide a bactericidal effect. We hypothesize that this may reduce the rate of infections development and protect cell viability. Aim: The aim of this study was two-fold—to create a stable APRF membrane treated with different concentrations of CHX (0.01% and 0.02%) and to monitor its effect on the viability of PDL cells in vitro. This benefits the introduction of a new protocol for APRF membrane production -CHX-PRF and enriches the available evidence on the effect of this antiseptic agent on PDL (Periodontal ligament) cells. Materials and methods: APRF membranes were prepared by the addition of two concentrations (0.01% and 0.02%) of CHX. Membranes without the antiseptic were also prepared and used as control samples. PDL cells were cultivated on the membranes for 72 h. Cell number and vitality were examined by fluorescent cell viability assays. Results: Our results demonstrated that a concentration of 0.01% CHX allowed the production of a stable APRF membrane. This concentration slightly reduced the viability of PDL cells to 96.7%, but significantly decreased the average number of cells attached to the membrane—149 ± 16.5 cells/field compared to controls −336 ± 26.9 cells/field. APRF-CHX 0.02% membranes were unstable, indicating a dose-dependent cytotoxic effect of CHX. Conclusions: The introduced novel protocol leads to the production of a new type of APRF membrane—CHX-PRF. The incorporation of an antiseptic into the APRF membrane can improve its bactericidal activity and might serve as an important step for the prevention of postoperative infections.https://www.mdpi.com/2079-4983/13/4/226ChlorhexidineAPRF membranevitalityPDL cells |
| spellingShingle | Tasho Gavrailov Ivan Chenchev Maria Gevezova Milena Draganova Victoria Sarafian Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated Membranes Journal of Functional Biomaterials Chlorhexidine APRF membrane vitality PDL cells |
| title | Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated Membranes |
| title_full | Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated Membranes |
| title_fullStr | Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated Membranes |
| title_full_unstemmed | Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated Membranes |
| title_short | Effect on Cellular Vitality In Vitro of Novel APRF-Chlorhexidine Treated Membranes |
| title_sort | effect on cellular vitality in vitro of novel aprf chlorhexidine treated membranes |
| topic | Chlorhexidine APRF membrane vitality PDL cells |
| url | https://www.mdpi.com/2079-4983/13/4/226 |
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