DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAY
Sclerotinia scletoriorum (Lib.) de Bary is a serious pathogen of carrots that can cause up to 50% losses of stored roots. Fungicides were found not to be completely effective for its control and due to residues they are not permitted for the use in storage. Biological control agents such as Pythium...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Life Sciences in Lublin - Publishing House
2018-11-01
|
| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
| Subjects: | |
| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/28 |
| _version_ | 1797394233977470976 |
|---|---|
| author | Anna Baturo-Cieśniewska Aleksander Łukanowski Katarzyna Koczwara Leszek Lenc |
| author_facet | Anna Baturo-Cieśniewska Aleksander Łukanowski Katarzyna Koczwara Leszek Lenc |
| author_sort | Anna Baturo-Cieśniewska |
| collection | DOAJ |
| description |
Sclerotinia scletoriorum (Lib.) de Bary is a serious pathogen of carrots that can cause up to 50% losses of stored roots. Fungicides were found not to be completely effective for its control and due to residues they are not permitted for the use in storage. Biological control agents such as Pythium oligandrum may be a chance. The aim of research was to determine the level of carrot infection with S. sclerotiorum during few-month storage with qPCR and the potential control ability of this pathogen by Polyversum WP containing oospores of P. oligandrum. We analyzed carrot roots in combinations treated with Polyversum WP on the field, fumigated with biopreparation before storage and untreated control. S. sclerotiorum developed on carrots treated and untreated with Polyversum WP. During the storage, pathogen was isolated from 44.4% samples and P. oligandrum from 53.8% ones, respectively in the range from 0.0001 to 130 200.0; pg and from 0.004 to 0.3440 pg per sample. The number of roots with S. sclerotiorum and degree of their infection increased with prolonged storage. Analyses suggest that Polyversum WP may potentially limit the growth of S. sclerotiorum.
|
| first_indexed | 2024-03-09T00:15:37Z |
| format | Article |
| id | doaj.art-ce3ae118055547dc851b3833a1173afe |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:15:37Z |
| publishDate | 2018-11-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-ce3ae118055547dc851b3833a1173afe2023-12-12T07:58:16ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052018-11-0117510.24326/asphc.2018.5.10DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAYAnna Baturo-Cieśniewska0Aleksander Łukanowski1Katarzyna Koczwara2Leszek Lenc3Laboratory of Phytopathology and Molecular Mycology, UTP University of Science and Technology, Kordeckiego 20, 85-225 BydgoszczLaboratory of Phytopathology and Molecular Mycology, UTP University of Science and Technology, Kordeckiego 20, 85-225 BydgoszczLaboratory of Phytopathology and Molecular Mycology, UTP University of Science and Technology, Kordeckiego 20, 85-225 BydgoszczLaboratory of Phytopathology and Molecular Mycology, UTP University of Science and Technology, Kordeckiego 20, 85-225 Bydgoszcz Sclerotinia scletoriorum (Lib.) de Bary is a serious pathogen of carrots that can cause up to 50% losses of stored roots. Fungicides were found not to be completely effective for its control and due to residues they are not permitted for the use in storage. Biological control agents such as Pythium oligandrum may be a chance. The aim of research was to determine the level of carrot infection with S. sclerotiorum during few-month storage with qPCR and the potential control ability of this pathogen by Polyversum WP containing oospores of P. oligandrum. We analyzed carrot roots in combinations treated with Polyversum WP on the field, fumigated with biopreparation before storage and untreated control. S. sclerotiorum developed on carrots treated and untreated with Polyversum WP. During the storage, pathogen was isolated from 44.4% samples and P. oligandrum from 53.8% ones, respectively in the range from 0.0001 to 130 200.0; pg and from 0.004 to 0.3440 pg per sample. The number of roots with S. sclerotiorum and degree of their infection increased with prolonged storage. Analyses suggest that Polyversum WP may potentially limit the growth of S. sclerotiorum. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/28carrotSclerotinia sclerotiorumPythium oligandrumstorage |
| spellingShingle | Anna Baturo-Cieśniewska Aleksander Łukanowski Katarzyna Koczwara Leszek Lenc DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAY Acta Scientiarum Polonorum: Hortorum Cultus carrot Sclerotinia sclerotiorum Pythium oligandrum storage |
| title | DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAY |
| title_full | DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAY |
| title_fullStr | DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAY |
| title_full_unstemmed | DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAY |
| title_short | DEVELOPMENT OF SCLEROTINIA SCLEROTIORUM (LIB.) DE BARY ON STORED CARROT TREATED WITH PYTHIUM OLIGANDRUM DRECHSLER DETERMINED BY QPCR ASSAY |
| title_sort | development of sclerotinia sclerotiorum lib de bary on stored carrot treated with pythium oligandrum drechsler determined by qpcr assay |
| topic | carrot Sclerotinia sclerotiorum Pythium oligandrum storage |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/28 |
| work_keys_str_mv | AT annabaturociesniewska developmentofsclerotiniasclerotiorumlibdebaryonstoredcarrottreatedwithpythiumoligandrumdrechslerdeterminedbyqpcrassay AT aleksanderłukanowski developmentofsclerotiniasclerotiorumlibdebaryonstoredcarrottreatedwithpythiumoligandrumdrechslerdeterminedbyqpcrassay AT katarzynakoczwara developmentofsclerotiniasclerotiorumlibdebaryonstoredcarrottreatedwithpythiumoligandrumdrechslerdeterminedbyqpcrassay AT leszeklenc developmentofsclerotiniasclerotiorumlibdebaryonstoredcarrottreatedwithpythiumoligandrumdrechslerdeterminedbyqpcrassay |