Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures
The primary objective of this work was to assess the effect of Bacillus amyloliquefaciens on the microbiota in polyculture tanks stocked with grass carp, gibel carp and sliver carp. The B. amyloliquefaciens showed high biosafety toward freshwater fish. The addition of B. amyloliquefaciens to the tan...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-11-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513421002386 |
| _version_ | 1819179071338184704 |
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| author | Yibin Yang Jingjin Xia Yongtao Liu Jing Dong Ning Xu Qiuhong Yang Shun Zhou Xiaohui Ai |
| author_facet | Yibin Yang Jingjin Xia Yongtao Liu Jing Dong Ning Xu Qiuhong Yang Shun Zhou Xiaohui Ai |
| author_sort | Yibin Yang |
| collection | DOAJ |
| description | The primary objective of this work was to assess the effect of Bacillus amyloliquefaciens on the microbiota in polyculture tanks stocked with grass carp, gibel carp and sliver carp. The B. amyloliquefaciens showed high biosafety toward freshwater fish. The addition of B. amyloliquefaciens to the tanks significantly reduced ammonia, total nitrogen, total phosphorus and chemical oxygen demand (p ＜ 0.05) although nitrites increased (p ＜ 0.05). Bacterial diversity in the tanks was greatest in the un-inoculated tanks where Proteobacteria, Actinobacteria, Cyanobacteria, Verrucomicrobia and Bacteroidetes dominated. In the presence of added B. amyloliquefaciens, Proteobacteria and Bacteroidetes levels increased while Actinobacteria decreased. The classes and genera between controls and treatment groups differed although Bacillus levels did not increase in the inoculated tanks. We conclude that supplementation with B. amyloliquefaciens in aquaculture water had many advantages but also potential safety risks. |
| first_indexed | 2024-12-22T21:52:37Z |
| format | Article |
| id | doaj.art-ce3e533a9bbd4b3e8f2eecfe2355bbac |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-12-22T21:52:37Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-ce3e533a9bbd4b3e8f2eecfe2355bbac2022-12-21T18:11:20ZengElsevierAquaculture Reports2352-51342021-11-0121100822Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish culturesYibin Yang0Jingjin Xia1Yongtao Liu2Jing Dong3Ning Xu4Qiuhong Yang5Shun Zhou6Xiaohui Ai7Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, PR ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, PR ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, PR ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, PR ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, PR ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, PR ChinaYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China; Key Laboratory of Control of Quality and Safety for Aquatic Products, Ministry of Agriculture, Beijing 100141, PR China; Corresponding author at: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, PR China.The primary objective of this work was to assess the effect of Bacillus amyloliquefaciens on the microbiota in polyculture tanks stocked with grass carp, gibel carp and sliver carp. The B. amyloliquefaciens showed high biosafety toward freshwater fish. The addition of B. amyloliquefaciens to the tanks significantly reduced ammonia, total nitrogen, total phosphorus and chemical oxygen demand (p ＜ 0.05) although nitrites increased (p ＜ 0.05). Bacterial diversity in the tanks was greatest in the un-inoculated tanks where Proteobacteria, Actinobacteria, Cyanobacteria, Verrucomicrobia and Bacteroidetes dominated. In the presence of added B. amyloliquefaciens, Proteobacteria and Bacteroidetes levels increased while Actinobacteria decreased. The classes and genera between controls and treatment groups differed although Bacillus levels did not increase in the inoculated tanks. We conclude that supplementation with B. amyloliquefaciens in aquaculture water had many advantages but also potential safety risks.http://www.sciencedirect.com/science/article/pii/S2352513421002386AmmoniaIllumina-MiSeqMicrobial diversityBio-safety |
| spellingShingle | Yibin Yang Jingjin Xia Yongtao Liu Jing Dong Ning Xu Qiuhong Yang Shun Zhou Xiaohui Ai Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures Aquaculture Reports Ammonia Illumina-MiSeq Microbial diversity Bio-safety |
| title | Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures |
| title_full | Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures |
| title_fullStr | Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures |
| title_full_unstemmed | Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures |
| title_short | Safety evaluation for the use of Bacillus amyloliquefaciens in freshwater fish cultures |
| title_sort | safety evaluation for the use of bacillus amyloliquefaciens in freshwater fish cultures |
| topic | Ammonia Illumina-MiSeq Microbial diversity Bio-safety |
| url | http://www.sciencedirect.com/science/article/pii/S2352513421002386 |
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