Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars
Three carbon-chain extension genes associated with fatty acid synthesis in upland cotton (<i>Gossypium hirsutum</i>), namely <i>GhKAR</i>, <i>GhHAD</i>, and <i>GhENR</i>, play important roles in oil accumulation in cotton seeds. In the present study, t...
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MDPI AG
2023-09-01
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| author | Li Liu Dan Wang Jinping Hua Xianhui Kong Xuwen Wang Juan Wang Aijun Si Fuxiang Zhao Wenhao Liu Yu Yu Zhiwen Chen |
| author_facet | Li Liu Dan Wang Jinping Hua Xianhui Kong Xuwen Wang Juan Wang Aijun Si Fuxiang Zhao Wenhao Liu Yu Yu Zhiwen Chen |
| author_sort | Li Liu |
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| description | Three carbon-chain extension genes associated with fatty acid synthesis in upland cotton (<i>Gossypium hirsutum</i>), namely <i>GhKAR</i>, <i>GhHAD</i>, and <i>GhENR</i>, play important roles in oil accumulation in cotton seeds. In the present study, these three genes were cloned and characterized. The expression patterns of <i>GhKAR</i>, <i>GhHAD</i>, and <i>GhENR</i> in the high seed oil content cultivars 10H1014 and 10H1041 differed somewhat compared with those of 10H1007 and 2074B with low seed oil content at different stages of seed development. <i>GhKAR</i> showed all three cultivars showed higher transcript levels than that of 2074B at 10-, 40-, and 45-days post anthesis (DPA). The expression pattern of <i>GhHAD</i> showed a lower transcript level than that of 2074B at both 10 and 30 DPA but a higher transcript level than that of 2074B at 40 DPA. <i>GhENR</i> showed a lower transcript level than that of 2074B at both 15 and 30 DPA. The highest transcript levels of <i>GhKAR</i> and <i>GhENR</i> were detected at 15 DPA in 10H1007, 10H1014, and 10H1041 compared with 2074B. From 5 to 45 DPA cotton seed, the oil content accumulated continuously in the developing seed. Oil accumulation reached a peak between 40 DPA and 45 DPA and slightly decreased in mature seed. In addition, <i>GhKAR</i> and <i>GhENR</i> showed different expression patterns in fiber and ovule development processes, in which they showed high expression levels at 20 DPA during the fiber elongation stage, but their expression level peaked at 15 DPA during ovule development processes. These two genes showed the lowest expression levels at the late seed maturation stage, while <i>GhHAD</i> showed a peak of 10 DPA in fiber development. Compared to 2074B, the oil contents of <i>GhKAR</i> and <i>GhENR</i> overexpression lines increased 1.05~1.08 folds. These results indicated that <i>GhHAD</i>, <i>GhENR</i>, and <i>GhKAR</i> were involved in both seed oil synthesis and fiber elongation with dual biological functions in cotton. |
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| spelling | doaj.art-ce3f2e069111404a860aa2712ec21b9d2023-11-19T14:54:23ZengMDPI AGPlants2223-77472023-09-011219343710.3390/plants12193437Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton CultivarsLi Liu0Dan Wang1Jinping Hua2Xianhui Kong3Xuwen Wang4Juan Wang5Aijun Si6Fuxiang Zhao7Wenhao Liu8Yu Yu9Zhiwen Chen10Cotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaLaboratory of Cotton Genetics, Genomics and Breeding/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis and Utilization of Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaLaboratory of Cotton Genetics, Genomics and Breeding/Beijing Key Laboratory of Crop Genetic Improvement/Key Laboratory of Crop Heterosis and Utilization of Ministry of Education, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, ChinaCotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaCotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaCotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaCotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaCotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaCotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaCotton Institute, Xinjiang Academy of Agricultural and Reclamation Science/Northwest Inland Region Key Laboratory of Cotton Biology and Genetic Breeding, Shihezi 832000, ChinaKey Laboratory of Graphene Forestry Application of National Forest and Grass Administration, Engineering Research Center of Coal-Based Ecological Carbon Sequestration Technology of the Ministry of Education, Shanxi Datong University, Datong 037009, ChinaThree carbon-chain extension genes associated with fatty acid synthesis in upland cotton (<i>Gossypium hirsutum</i>), namely <i>GhKAR</i>, <i>GhHAD</i>, and <i>GhENR</i>, play important roles in oil accumulation in cotton seeds. In the present study, these three genes were cloned and characterized. The expression patterns of <i>GhKAR</i>, <i>GhHAD</i>, and <i>GhENR</i> in the high seed oil content cultivars 10H1014 and 10H1041 differed somewhat compared with those of 10H1007 and 2074B with low seed oil content at different stages of seed development. <i>GhKAR</i> showed all three cultivars showed higher transcript levels than that of 2074B at 10-, 40-, and 45-days post anthesis (DPA). The expression pattern of <i>GhHAD</i> showed a lower transcript level than that of 2074B at both 10 and 30 DPA but a higher transcript level than that of 2074B at 40 DPA. <i>GhENR</i> showed a lower transcript level than that of 2074B at both 15 and 30 DPA. The highest transcript levels of <i>GhKAR</i> and <i>GhENR</i> were detected at 15 DPA in 10H1007, 10H1014, and 10H1041 compared with 2074B. From 5 to 45 DPA cotton seed, the oil content accumulated continuously in the developing seed. Oil accumulation reached a peak between 40 DPA and 45 DPA and slightly decreased in mature seed. In addition, <i>GhKAR</i> and <i>GhENR</i> showed different expression patterns in fiber and ovule development processes, in which they showed high expression levels at 20 DPA during the fiber elongation stage, but their expression level peaked at 15 DPA during ovule development processes. These two genes showed the lowest expression levels at the late seed maturation stage, while <i>GhHAD</i> showed a peak of 10 DPA in fiber development. Compared to 2074B, the oil contents of <i>GhKAR</i> and <i>GhENR</i> overexpression lines increased 1.05~1.08 folds. These results indicated that <i>GhHAD</i>, <i>GhENR</i>, and <i>GhKAR</i> were involved in both seed oil synthesis and fiber elongation with dual biological functions in cotton.https://www.mdpi.com/2223-7747/12/19/3437fatty acidsfiber developmentgene expressionseed oil contentupland cotton (<i>Gossypium hirsutum</i>) |
| spellingShingle | Li Liu Dan Wang Jinping Hua Xianhui Kong Xuwen Wang Juan Wang Aijun Si Fuxiang Zhao Wenhao Liu Yu Yu Zhiwen Chen Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars Plants fatty acids fiber development gene expression seed oil content upland cotton (<i>Gossypium hirsutum</i>) |
| title | Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars |
| title_full | Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars |
| title_fullStr | Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars |
| title_full_unstemmed | Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars |
| title_short | Genetic and Morpho-Physiological Differences among Transgenic and No-Transgenic Cotton Cultivars |
| title_sort | genetic and morpho physiological differences among transgenic and no transgenic cotton cultivars |
| topic | fatty acids fiber development gene expression seed oil content upland cotton (<i>Gossypium hirsutum</i>) |
| url | https://www.mdpi.com/2223-7747/12/19/3437 |
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