Synthesis of Peptides from Glycine on Anatases with Different Crystal Facets
Semiconductor minerals are widely present on the surface of Earth, but their roles in the process of peptide formation from amino acids are less studied, especially the role of different crystal facets in the origin of life. In this research, High Performance Liquid Chromatography (HPLC), thermograv...
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/2073-4352/13/7/1113 |
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| author | Jingping Chen Xiaomei Zhong Haiyan Wu Zeling Liu Xiaoqin Nie Lei Zhou Hongfu Wei Wenyuan Hu Mingxue Liu |
| author_facet | Jingping Chen Xiaomei Zhong Haiyan Wu Zeling Liu Xiaoqin Nie Lei Zhou Hongfu Wei Wenyuan Hu Mingxue Liu |
| author_sort | Jingping Chen |
| collection | DOAJ |
| description | Semiconductor minerals are widely present on the surface of Earth, but their roles in the process of peptide formation from amino acids are less studied, especially the role of different crystal facets in the origin of life. In this research, High Performance Liquid Chromatography (HPLC), thermogravimetric analysis (TA/DTA), Nuclear Magnetic Resonance (NMR) and simulation calculations were used to study the condensation of glycine on the surface of anatase with (001) crystal facets and ordinary anatase as well as the reaction mechanism. Combined with TA/DTA and heating experiments (80–130 °C), it was found that anatase with (001) crystal facets and ordinary anatase could both catalyze the condensation of glycine to form corresponding oligopeptides (mainly DKP, Gly<sub>2</sub> and Gly<sub>3</sub>). Anatase with (001) crystal facets shows better catalytic effect, which can reduce the condensation temperature of glycine to 90 °C. With the increase in temperature, the condensation efficiency of anatase with (001) crystal facets for Gly<sub>2</sub> is relatively higher, and the maximum yield is about 0.20 mg/m<sup>2</sup>. The condensation efficiency of ordinary anatase for Gly<sub>3</sub> is relatively higher, and the maximum yield is about 0.28 mg/m<sup>2</sup>. The results of FTIR and simulation calculation show that the electron density of the carboxyl group changes after glycine is adsorbed on the surface of anatase, which is easily subject to the nucleophilic attack of amino groups to promote the condensation reaction. These results can provide reference for the research of condensation of small biomolecules on semiconductor mineral surfaces in the origin of life. |
| first_indexed | 2024-03-11T01:11:06Z |
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| language | English |
| last_indexed | 2024-03-11T01:11:06Z |
| publishDate | 2023-07-01 |
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| record_format | Article |
| series | Crystals |
| spelling | doaj.art-ce3f842daf77449b90e5156e06b9fcbc2023-11-18T18:54:41ZengMDPI AGCrystals2073-43522023-07-01137111310.3390/cryst13071113Synthesis of Peptides from Glycine on Anatases with Different Crystal FacetsJingping Chen0Xiaomei Zhong1Haiyan Wu2Zeling Liu3Xiaoqin Nie4Lei Zhou5Hongfu Wei6Wenyuan Hu7Mingxue Liu8School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of National Defense Science and Technology, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSemiconductor minerals are widely present on the surface of Earth, but their roles in the process of peptide formation from amino acids are less studied, especially the role of different crystal facets in the origin of life. In this research, High Performance Liquid Chromatography (HPLC), thermogravimetric analysis (TA/DTA), Nuclear Magnetic Resonance (NMR) and simulation calculations were used to study the condensation of glycine on the surface of anatase with (001) crystal facets and ordinary anatase as well as the reaction mechanism. Combined with TA/DTA and heating experiments (80–130 °C), it was found that anatase with (001) crystal facets and ordinary anatase could both catalyze the condensation of glycine to form corresponding oligopeptides (mainly DKP, Gly<sub>2</sub> and Gly<sub>3</sub>). Anatase with (001) crystal facets shows better catalytic effect, which can reduce the condensation temperature of glycine to 90 °C. With the increase in temperature, the condensation efficiency of anatase with (001) crystal facets for Gly<sub>2</sub> is relatively higher, and the maximum yield is about 0.20 mg/m<sup>2</sup>. The condensation efficiency of ordinary anatase for Gly<sub>3</sub> is relatively higher, and the maximum yield is about 0.28 mg/m<sup>2</sup>. The results of FTIR and simulation calculation show that the electron density of the carboxyl group changes after glycine is adsorbed on the surface of anatase, which is easily subject to the nucleophilic attack of amino groups to promote the condensation reaction. These results can provide reference for the research of condensation of small biomolecules on semiconductor mineral surfaces in the origin of life.https://www.mdpi.com/2073-4352/13/7/1113glycinepeptideanatasecrystal facetscondensationreaction mechanism |
| spellingShingle | Jingping Chen Xiaomei Zhong Haiyan Wu Zeling Liu Xiaoqin Nie Lei Zhou Hongfu Wei Wenyuan Hu Mingxue Liu Synthesis of Peptides from Glycine on Anatases with Different Crystal Facets Crystals glycine peptide anatase crystal facets condensation reaction mechanism |
| title | Synthesis of Peptides from Glycine on Anatases with Different Crystal Facets |
| title_full | Synthesis of Peptides from Glycine on Anatases with Different Crystal Facets |
| title_fullStr | Synthesis of Peptides from Glycine on Anatases with Different Crystal Facets |
| title_full_unstemmed | Synthesis of Peptides from Glycine on Anatases with Different Crystal Facets |
| title_short | Synthesis of Peptides from Glycine on Anatases with Different Crystal Facets |
| title_sort | synthesis of peptides from glycine on anatases with different crystal facets |
| topic | glycine peptide anatase crystal facets condensation reaction mechanism |
| url | https://www.mdpi.com/2073-4352/13/7/1113 |
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