Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18.
Francisella tularensis is a highly infectious human intracellular pathogen that is the causative agent of tularemia. It occurs in several major subtypes, including the live vaccine strain holarctica (type B). F. tularensis is classified as category A biodefense agent in part because a relatively sma...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2561293?pdf=render |
| _version_ | 1818899522271576064 |
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| author | Daniela Puiu Steven L Salzberg |
| author_facet | Daniela Puiu Steven L Salzberg |
| author_sort | Daniela Puiu |
| collection | DOAJ |
| description | Francisella tularensis is a highly infectious human intracellular pathogen that is the causative agent of tularemia. It occurs in several major subtypes, including the live vaccine strain holarctica (type B). F. tularensis is classified as category A biodefense agent in part because a relatively small number of organisms can cause severe illness. Three complete genomes of subspecies holarctica have been sequenced and deposited in public archives, of which OSU18 was the first and the only strain for which a scientific publication has appeared. We re-assembled the OSU18 strain using both de novo and comparative assembly techniques, and found that the published sequence has two large inversion mis-assemblies. We generated a corrected assembly of the entire genome along with detailed information on the placement of individual reads within the assembly. This assembly will provide a more accurate basis for future comparative studies of this pathogen. |
| first_indexed | 2024-12-19T19:49:18Z |
| format | Article |
| id | doaj.art-ce43e6dcd89946d49c4525bfc9e143b0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T19:49:18Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ce43e6dcd89946d49c4525bfc9e143b02022-12-21T20:08:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-01310e342710.1371/journal.pone.0003427Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18.Daniela PuiuSteven L SalzbergFrancisella tularensis is a highly infectious human intracellular pathogen that is the causative agent of tularemia. It occurs in several major subtypes, including the live vaccine strain holarctica (type B). F. tularensis is classified as category A biodefense agent in part because a relatively small number of organisms can cause severe illness. Three complete genomes of subspecies holarctica have been sequenced and deposited in public archives, of which OSU18 was the first and the only strain for which a scientific publication has appeared. We re-assembled the OSU18 strain using both de novo and comparative assembly techniques, and found that the published sequence has two large inversion mis-assemblies. We generated a corrected assembly of the entire genome along with detailed information on the placement of individual reads within the assembly. This assembly will provide a more accurate basis for future comparative studies of this pathogen.http://europepmc.org/articles/PMC2561293?pdf=render |
| spellingShingle | Daniela Puiu Steven L Salzberg Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18. PLoS ONE |
| title | Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18. |
| title_full | Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18. |
| title_fullStr | Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18. |
| title_full_unstemmed | Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18. |
| title_short | Re-assembly of the genome of Francisella tularensis subsp. holarctica OSU18. |
| title_sort | re assembly of the genome of francisella tularensis subsp holarctica osu18 |
| url | http://europepmc.org/articles/PMC2561293?pdf=render |
| work_keys_str_mv | AT danielapuiu reassemblyofthegenomeoffrancisellatularensissubspholarcticaosu18 AT stevenlsalzberg reassemblyofthegenomeoffrancisellatularensissubspholarcticaosu18 |