Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture

Bacterial diversity of phototrophic (PAb) and heterotrophic (Hb) biofilms was studied over time (0, 15 and 30 days of culture) using high throughput sequencing and considering the V3 and V4 hypervariable regions of the 16S rRNA gene. Bacterial composition in terms of relative abundance and diversit...

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Main Authors: Luis Rafael Martínez-Córdova, Marcel Martínez-Porchas, Francisco Vargas-Albores, Anselmo Miranda-Baeza, Mauricio Coelho-Emerenciano, Marco Antonio Porchas-Cornejo, Edilmar Cortes-Jacinto, Miguel Ángel Mazorra-Manzano
Format: Article
Language:English
Published: Universidad Autónoma de Baja California 2018-12-01
Series:Ciencias Marinas
Online Access:https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829
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author Luis Rafael Martínez-Córdova
Marcel Martínez-Porchas
Francisco Vargas-Albores
Anselmo Miranda-Baeza
Mauricio Coelho-Emerenciano
Marco Antonio Porchas-Cornejo
Edilmar Cortes-Jacinto
Miguel Ángel Mazorra-Manzano
author_facet Luis Rafael Martínez-Córdova
Marcel Martínez-Porchas
Francisco Vargas-Albores
Anselmo Miranda-Baeza
Mauricio Coelho-Emerenciano
Marco Antonio Porchas-Cornejo
Edilmar Cortes-Jacinto
Miguel Ángel Mazorra-Manzano
author_sort Luis Rafael Martínez-Córdova
collection DOAJ
description Bacterial diversity of phototrophic (PAb) and heterotrophic (Hb) biofilms was studied over time (0, 15 and 30 days of culture) using high throughput sequencing and considering the V3 and V4 hypervariable regions of the 16S rRNA gene. Bacterial composition in terms of relative abundance and diversity showed slight changes during the trial. Proteobacteria was the most abundant phylum in both biofilms during the experimental period. Chlamidiae-Verrucomicrobia, Bacteriodetes, and Planctomycetes were also abundant phyla in the PAb, whereas Planctomycetes, Bacteriodetes, Actinobacteria, and Chlamydiae were the abundant phyla in the Hb. Of the reads assigned up to species level, a total of 27 heterotrophic and autotrophic species were detected in both biofilms, most of them associated with the metabolism of nitrogenous and sulfurous metabolites and organic matter, and the rest with the structural functions in the biofilm. This is the first time some of these species have been reportedly detected in these biofilms or in the marine environment. Results suggest complex interaction networks in microbial conglomerates formed in biofilms, in which bacterial populations seem to play important metabolic and physiological roles.
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spelling doaj.art-ce44130c8d3249338ce046579f23db012024-03-03T17:38:18ZengUniversidad Autónoma de Baja CaliforniaCiencias Marinas0185-38802395-90532018-12-0144410.7773/cm.v44i4.2829Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquacultureLuis Rafael Martínez-Córdova0Marcel Martínez-Porchas1Francisco Vargas-Albores2Anselmo Miranda-Baeza3Mauricio Coelho-Emerenciano4Marco Antonio Porchas-Cornejo5Edilmar Cortes-Jacinto6Miguel Ángel Mazorra-Manzano7Departamento de Investigaciones Científicas y Tecnológicas de la Universidad de SonoraCentro de Investigación en Alimentación y DesarrolloCentro de Investigación en Alimentación y DesarrolloUniversidad Estatal de SonoraUniversidade Estadual de Santa CatarinaCentro de Investigaciones Biológicas del Noroeste, Unidad GuaymasCentro de Investigaciones Biológicas del Noroeste, Unidad La PazCentro de Investigación en Alimentación y Desarrollo Bacterial diversity of phototrophic (PAb) and heterotrophic (Hb) biofilms was studied over time (0, 15 and 30 days of culture) using high throughput sequencing and considering the V3 and V4 hypervariable regions of the 16S rRNA gene. Bacterial composition in terms of relative abundance and diversity showed slight changes during the trial. Proteobacteria was the most abundant phylum in both biofilms during the experimental period. Chlamidiae-Verrucomicrobia, Bacteriodetes, and Planctomycetes were also abundant phyla in the PAb, whereas Planctomycetes, Bacteriodetes, Actinobacteria, and Chlamydiae were the abundant phyla in the Hb. Of the reads assigned up to species level, a total of 27 heterotrophic and autotrophic species were detected in both biofilms, most of them associated with the metabolism of nitrogenous and sulfurous metabolites and organic matter, and the rest with the structural functions in the biofilm. This is the first time some of these species have been reportedly detected in these biofilms or in the marine environment. Results suggest complex interaction networks in microbial conglomerates formed in biofilms, in which bacterial populations seem to play important metabolic and physiological roles. https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829
spellingShingle Luis Rafael Martínez-Córdova
Marcel Martínez-Porchas
Francisco Vargas-Albores
Anselmo Miranda-Baeza
Mauricio Coelho-Emerenciano
Marco Antonio Porchas-Cornejo
Edilmar Cortes-Jacinto
Miguel Ángel Mazorra-Manzano
Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
Ciencias Marinas
title Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_full Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_fullStr Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_full_unstemmed Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_short Taxonomic profile of bacterial communities detected with 16S-rRNA in mature phototrophic and heterotrophic marine biofilms used for aquaculture
title_sort taxonomic profile of bacterial communities detected with 16s rrna in mature phototrophic and heterotrophic marine biofilms used for aquaculture
url https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/2829
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