Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactor
Abstract The bubble column reactor of 10 and 20 L capacity was designed to bio-mitigate 10% CO2 (g) with 90% air utilizing thermophilic bacteria (Bacillus cereus SSLMC2). The maximum biomass yield during the growth phase was obtained as 9.14 and 10.78 g L−1 for 10 and 20 L capacity, respectively. Th...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-56965-8 |
| _version_ | 1797259311089451008 |
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| author | Rachael J. Barla Smita Raghuvanshi Suresh Gupta |
| author_facet | Rachael J. Barla Smita Raghuvanshi Suresh Gupta |
| author_sort | Rachael J. Barla |
| collection | DOAJ |
| description | Abstract The bubble column reactor of 10 and 20 L capacity was designed to bio-mitigate 10% CO2 (g) with 90% air utilizing thermophilic bacteria (Bacillus cereus SSLMC2). The maximum biomass yield during the growth phase was obtained as 9.14 and 10.78 g L−1 for 10 and 20 L capacity, respectively. The maximum removal efficiency for CO2 (g) was obtained as 56% and 85% for the 10 and 20 L reactors, respectively. The FT-IR and GC–MS examination of the extracellular and intracellular samples identified value-added products such as carboxylic acid, fatty alcohols, and hydrocarbons produced during the process. The total carbon balance for CO2 utilization in different forms confirmed that B. cereus SSLMC2 utilized 1646.54 g C in 10 L and 1587 g of C in 20 L reactor out of 1696.13 g of total carbon feed. The techno-economic assessment established that the capital investment required was $286.21 and $289.08 per reactor run of 11 days and $0.167 and $0.187 per gram of carbon treated for 10 and 20 L reactors, respectively. The possible mechanism pathways for bio-mitigating CO2 (g) by B. cereus SSLMC2 were also presented utilizing the energy reactions. Hence, the work presents the novelty of utilizing thermophilic bacteria and a bubble column bioreactor for CO2 (g) bio-mitigation. |
| first_indexed | 2024-04-24T23:07:24Z |
| format | Article |
| id | doaj.art-ce44d31f151c4e31893ce842e404416e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T23:07:24Z |
| publishDate | 2024-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ce44d31f151c4e31893ce842e404416e2024-03-17T12:26:28ZengNature PortfolioScientific Reports2045-23222024-03-0114111610.1038/s41598-024-56965-8Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactorRachael J. Barla0Smita Raghuvanshi1Suresh Gupta2Faculty Division-1, Department of Chemical Engineering, Birla Institute of Technology and Science (BITS), BITS PILANIFaculty Division-1, Department of Chemical Engineering, Birla Institute of Technology and Science (BITS), BITS PILANIFaculty Division-1, Department of Chemical Engineering, Birla Institute of Technology and Science (BITS), BITS PILANIAbstract The bubble column reactor of 10 and 20 L capacity was designed to bio-mitigate 10% CO2 (g) with 90% air utilizing thermophilic bacteria (Bacillus cereus SSLMC2). The maximum biomass yield during the growth phase was obtained as 9.14 and 10.78 g L−1 for 10 and 20 L capacity, respectively. The maximum removal efficiency for CO2 (g) was obtained as 56% and 85% for the 10 and 20 L reactors, respectively. The FT-IR and GC–MS examination of the extracellular and intracellular samples identified value-added products such as carboxylic acid, fatty alcohols, and hydrocarbons produced during the process. The total carbon balance for CO2 utilization in different forms confirmed that B. cereus SSLMC2 utilized 1646.54 g C in 10 L and 1587 g of C in 20 L reactor out of 1696.13 g of total carbon feed. The techno-economic assessment established that the capital investment required was $286.21 and $289.08 per reactor run of 11 days and $0.167 and $0.187 per gram of carbon treated for 10 and 20 L reactors, respectively. The possible mechanism pathways for bio-mitigating CO2 (g) by B. cereus SSLMC2 were also presented utilizing the energy reactions. Hence, the work presents the novelty of utilizing thermophilic bacteria and a bubble column bioreactor for CO2 (g) bio-mitigation.https://doi.org/10.1038/s41598-024-56965-8 |
| spellingShingle | Rachael J. Barla Smita Raghuvanshi Suresh Gupta Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactor Scientific Reports |
| title | Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactor |
| title_full | Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactor |
| title_fullStr | Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactor |
| title_full_unstemmed | Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactor |
| title_short | Reforming CO2 bio-mitigation utilizing Bacillus cereus from hypersaline realms in pilot-scale bubble column bioreactor |
| title_sort | reforming co2 bio mitigation utilizing bacillus cereus from hypersaline realms in pilot scale bubble column bioreactor |
| url | https://doi.org/10.1038/s41598-024-56965-8 |
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