SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL
Microneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC-3) is the protein that plays an important role in the invasion proccess during cell infection as a mediator attachment parasite to the host cell. The aim of t...
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| Format: | Article |
| Language: | English |
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Jenderal Soedirman University
2011-05-01
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| Series: | Molekul |
| Subjects: | |
| Online Access: | http://www.jmolekul.com/?download=6.1.10.pdf |
| _version_ | 1818962949388107776 |
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| author | Diana Indrasanti Aris Haryanto Wayan T. Artama |
| author_facet | Diana Indrasanti Aris Haryanto Wayan T. Artama |
| author_sort | Diana Indrasanti |
| collection | DOAJ |
| description | Microneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC-3) is the protein that plays an important role in the invasion proccess during cell infection as a mediator attachment parasite to the host cell. The aim of this research is to clone mic3 (gene encoding for MIC-3) of T. gondii from local isolate using recombinant DNA technology by cloning mic3 in an expression vector. Deoxyribonucleic acid (DNA) from T. gondii tachyzoites was amplified by PuRe Taq RTG-PCR Beads using mic3 specific primers. Amplified DNA was double digested using EcoRV and HindIII restriction endonucleases and then purified using EZ-10 spin coloumn purification kit. The mic3 DNA was ligated into pET-32a(+) expression vector and transformated into Escherichia coli BL21. The results showed that recombinant mic3 gene 4.2 kDa has been successfully performed by cloning gene encoding for MIC-3 protein of T. gondii local isolate into pET-32a(+) and transformed to E. coli BL21. |
| first_indexed | 2024-12-20T12:37:27Z |
| format | Article |
| id | doaj.art-ce45abd5580b4901b8a7f4643085bbc9 |
| institution | Directory Open Access Journal |
| issn | 1907-9761 1907-9761 |
| language | English |
| last_indexed | 2024-12-20T12:37:27Z |
| publishDate | 2011-05-01 |
| publisher | Jenderal Soedirman University |
| record_format | Article |
| series | Molekul |
| spelling | doaj.art-ce45abd5580b4901b8a7f4643085bbc92022-12-21T19:40:35ZengJenderal Soedirman UniversityMolekul1907-97611907-97612011-05-01611018SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKALDiana Indrasanti0Aris Haryanto1Wayan T. Artama2Laboratorium Kesehatan Ternak, Fakultas Peternakan, UNSOED Purwokertoaboratorium Biokimia, Fakultas Kedokteran Hewan, UGM, Yogyakartaaboratorium Biokimia, Fakultas Kedokteran Hewan, UGM, YogyakartaMicroneme protein (MIC) is one of proteins that belongs to excretory-secretory antigens (ESAs) of Toxoplasma gondii. Microneme 3 protein (MIC-3) is the protein that plays an important role in the invasion proccess during cell infection as a mediator attachment parasite to the host cell. The aim of this research is to clone mic3 (gene encoding for MIC-3) of T. gondii from local isolate using recombinant DNA technology by cloning mic3 in an expression vector. Deoxyribonucleic acid (DNA) from T. gondii tachyzoites was amplified by PuRe Taq RTG-PCR Beads using mic3 specific primers. Amplified DNA was double digested using EcoRV and HindIII restriction endonucleases and then purified using EZ-10 spin coloumn purification kit. The mic3 DNA was ligated into pET-32a(+) expression vector and transformated into Escherichia coli BL21. The results showed that recombinant mic3 gene 4.2 kDa has been successfully performed by cloning gene encoding for MIC-3 protein of T. gondii local isolate into pET-32a(+) and transformed to E. coli BL21.http://www.jmolekul.com/?download=6.1.10.pdfToxoplasma gondiicloningMIC-3 protein |
| spellingShingle | Diana Indrasanti Aris Haryanto Wayan T. Artama SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL Molekul Toxoplasma gondii cloning MIC-3 protein |
| title | SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL |
| title_full | SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL |
| title_fullStr | SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL |
| title_full_unstemmed | SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL |
| title_short | SUBKLONING DAN ISOLASI GEN PENYANDI MIKRONEMA 3 (MIC-3) Toxoplasma gondii ISOLAT LOKAL |
| title_sort | subkloning dan isolasi gen penyandi mikronema 3 mic 3 toxoplasma gondii isolat lokal |
| topic | Toxoplasma gondii cloning MIC-3 protein |
| url | http://www.jmolekul.com/?download=6.1.10.pdf |
| work_keys_str_mv | AT dianaindrasanti subkloningdanisolasigenpenyandimikronema3mic3toxoplasmagondiiisolatlokal AT arisharyanto subkloningdanisolasigenpenyandimikronema3mic3toxoplasmagondiiisolatlokal AT wayantartama subkloningdanisolasigenpenyandimikronema3mic3toxoplasmagondiiisolatlokal |