Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force
Cell separation has become @important in biological and medical applications. Dielectrophoresis (DEP) is widely used due to the advantages it offers, such as the lack of a requirement for biological markers and the fact that it involves no damage to cells or particles. This study aimed to report a n...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/1/117 |
| _version_ | 1797491847947354112 |
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| author | Xiaohong Li Junping Duan Zeng Qu Jiayun Wang Miaomiao Ji Binzhen Zhang |
| author_facet | Xiaohong Li Junping Duan Zeng Qu Jiayun Wang Miaomiao Ji Binzhen Zhang |
| author_sort | Xiaohong Li |
| collection | DOAJ |
| description | Cell separation has become @important in biological and medical applications. Dielectrophoresis (DEP) is widely used due to the advantages it offers, such as the lack of a requirement for biological markers and the fact that it involves no damage to cells or particles. This study aimed to report a novel approach combining 3D sidewall electrodes and contraction/expansion (CEA) structures to separate three kinds of particles with different sizes or dielectric properties continuously. The separation was achieved through the interaction between electrophoretic forces and inertia forces. The CEA channel was capable of sorting particles with different sizes due to inertial forces, and also enhanced the nonuniformity of the electric field. The 3D electrodes generated a non-uniform electric field at the same height as the channels, which increased the action range of the DEP force. Finite element simulations using the commercial software, COMSOL Multiphysics 5.4, were performed to determine the flow field distributions, electric field distributions, and particle trajectories. The separation experiments were assessed by separating 4 µm polystyrene (PS) particles from 20 µm PS particles at different flow rates by experiencing positive and negative DEP. Subsequently, the sorting performances of the 4 µm PS particles, 20 µm PS particles, and 4 µm silica particles with different solution conductivities were observed. Both the numerical simulations and the practical particle separation displayed high separating efficiency (separation of 4 µm PS particles, 94.2%; separation of 20 µm PS particles, 92.1%; separation of 4 µm Silica particles, 95.3%). The proposed approach is expected to open a new approach to cell sorting and separating. |
| first_indexed | 2024-03-10T00:55:09Z |
| format | Article |
| id | doaj.art-ce46bb74f5854490836a76691fe675bf |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T00:55:09Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-ce46bb74f5854490836a76691fe675bf2023-11-23T14:45:13ZengMDPI AGMicromachines2072-666X2022-01-0113111710.3390/mi13010117Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia ForceXiaohong Li0Junping Duan1Zeng Qu2Jiayun Wang3Miaomiao Ji4Binzhen Zhang5Key Laboratory of Instrumentation Science & Dynamic Measurement Ministry of Education, Micro Nano Technology Research Center, North University of China, Taiyuan 030051, ChinaKey Laboratory of Instrumentation Science & Dynamic Measurement Ministry of Education, Micro Nano Technology Research Center, North University of China, Taiyuan 030051, ChinaKey Laboratory of Instrumentation Science & Dynamic Measurement Ministry of Education, Micro Nano Technology Research Center, North University of China, Taiyuan 030051, ChinaKey Laboratory of Instrumentation Science & Dynamic Measurement Ministry of Education, Micro Nano Technology Research Center, North University of China, Taiyuan 030051, ChinaKey Laboratory of Instrumentation Science & Dynamic Measurement Ministry of Education, Micro Nano Technology Research Center, North University of China, Taiyuan 030051, ChinaKey Laboratory of Instrumentation Science & Dynamic Measurement Ministry of Education, Micro Nano Technology Research Center, North University of China, Taiyuan 030051, ChinaCell separation has become @important in biological and medical applications. Dielectrophoresis (DEP) is widely used due to the advantages it offers, such as the lack of a requirement for biological markers and the fact that it involves no damage to cells or particles. This study aimed to report a novel approach combining 3D sidewall electrodes and contraction/expansion (CEA) structures to separate three kinds of particles with different sizes or dielectric properties continuously. The separation was achieved through the interaction between electrophoretic forces and inertia forces. The CEA channel was capable of sorting particles with different sizes due to inertial forces, and also enhanced the nonuniformity of the electric field. The 3D electrodes generated a non-uniform electric field at the same height as the channels, which increased the action range of the DEP force. Finite element simulations using the commercial software, COMSOL Multiphysics 5.4, were performed to determine the flow field distributions, electric field distributions, and particle trajectories. The separation experiments were assessed by separating 4 µm polystyrene (PS) particles from 20 µm PS particles at different flow rates by experiencing positive and negative DEP. Subsequently, the sorting performances of the 4 µm PS particles, 20 µm PS particles, and 4 µm silica particles with different solution conductivities were observed. Both the numerical simulations and the practical particle separation displayed high separating efficiency (separation of 4 µm PS particles, 94.2%; separation of 20 µm PS particles, 92.1%; separation of 4 µm Silica particles, 95.3%). The proposed approach is expected to open a new approach to cell sorting and separating.https://www.mdpi.com/2072-666X/13/1/117dielectrophoresis3D electrodesinertialmicrofluidic chipparticle sorting |
| spellingShingle | Xiaohong Li Junping Duan Zeng Qu Jiayun Wang Miaomiao Ji Binzhen Zhang Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force Micromachines dielectrophoresis 3D electrodes inertial microfluidic chip particle sorting |
| title | Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force |
| title_full | Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force |
| title_fullStr | Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force |
| title_full_unstemmed | Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force |
| title_short | Continuous Particle Separation Driven by 3D Ag-PDMS Electrodes with Dielectric Electrophoretic Force Coupled with Inertia Force |
| title_sort | continuous particle separation driven by 3d ag pdms electrodes with dielectric electrophoretic force coupled with inertia force |
| topic | dielectrophoresis 3D electrodes inertial microfluidic chip particle sorting |
| url | https://www.mdpi.com/2072-666X/13/1/117 |
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