Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling
Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to invest...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=2;spage=315;epage=323;aulast=Wei |
| _version_ | 1818294274024800256 |
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| author | Shuai Wei Xue-Zhen Liang Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang |
| author_facet | Shuai Wei Xue-Zhen Liang Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang |
| author_sort | Shuai Wei |
| collection | DOAJ |
| description | Sensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the di?erences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between sensory and motor nerves in Wallerian degeneration and the characteristics of peripheral nerve regeneration. The study was approved by the Ethics Committee of the Chinese PLA General Hospital, China (approval No. 2016-x9-07) in September 2016. |
| first_indexed | 2024-12-13T03:29:08Z |
| format | Article |
| id | doaj.art-ce4aa88ef3ea4f09ae3f77af32a4ef29 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-13T03:29:08Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-ce4aa88ef3ea4f09ae3f77af32a4ef292022-12-22T00:01:13ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742020-01-0115231532310.4103/1673-5374.265556Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profilingShuai WeiXue-Zhen LiangQian HuWei-Shan WangWen-Jing XuXiao-Qing ChengJiang PengQuan-Yi GuoShu-Yun LiuWen JiangXiao DingGong-Hai HanPing LiuChen-Hui ShiYu WangSensory and motor nerve fibers of peripheral nerves have different anatomies and regeneration functions after injury. To gain a clear understanding of the biological processes behind these differences, we used a labeling technique termed isobaric tags for relative and absolute quantitation to investigate the protein profiles of spinal nerve tissues from Sprague-Dawley rats. In response to Wallerian degeneration, a total of 626 proteins were screened in sensory nerves, of which 368 were upregulated and 258 were downregulated. In addition, 637 proteins were screened in motor nerves, of which 372 were upregulated and 265 were downregulated. All identified proteins were analyzed using the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis of bioinformatics, and the presence of several key proteins closely related to Wallerian degeneration were tested and verified using quantitative real-time polymerase chain reaction analyses. The differentially expressed proteins only identified in the sensory nerves were mainly relevant to various biological processes that included cell-cell adhesion, carbohydrate metabolic processes and cell adhesion, whereas differentially expressed proteins only identified in the motor nerves were mainly relevant to biological processes associated with the glycolytic process, cell redox homeostasis, and protein folding. In the aspect of the cellular component, the differentially expressed proteins in the sensory and motor nerves were commonly related to extracellular exosomes, the myelin sheath, and focal adhesion. According to the Kyoto Encyclopedia of Genes and Genomes, the differentially expressed proteins identified are primarily related to various types of metabolic pathways. In conclusion, the present study screened differentially expressed proteins to reveal more about the di?erences and similarities between sensory and motor nerves during Wallerian degeneration. The present findings could provide a reference point for a future investigation into the differences between sensory and motor nerves in Wallerian degeneration and the characteristics of peripheral nerve regeneration. The study was approved by the Ethics Committee of the Chinese PLA General Hospital, China (approval No. 2016-x9-07) in September 2016.http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=2;spage=315;epage=323;aulast=Weigene ontology; Kyoto Encyclopedia of Genes and Genomes; isobaric tags for relative and absolute quantitation; motor nerve; proteomics; sensory nerve; spinal nerve; Wallerian degeneration |
| spellingShingle | Shuai Wei Xue-Zhen Liang Qian Hu Wei-Shan Wang Wen-Jing Xu Xiao-Qing Cheng Jiang Peng Quan-Yi Guo Shu-Yun Liu Wen Jiang Xiao Ding Gong-Hai Han Ping Liu Chen-Hui Shi Yu Wang Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling Neural Regeneration Research gene ontology; Kyoto Encyclopedia of Genes and Genomes; isobaric tags for relative and absolute quantitation; motor nerve; proteomics; sensory nerve; spinal nerve; Wallerian degeneration |
| title | Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling |
| title_full | Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling |
| title_fullStr | Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling |
| title_full_unstemmed | Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling |
| title_short | Different protein expression patterns in rat spinal nerves during Wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling |
| title_sort | different protein expression patterns in rat spinal nerves during wallerian degeneration assessed using isobaric tags for relative and absolute quantitation proteomics profiling |
| topic | gene ontology; Kyoto Encyclopedia of Genes and Genomes; isobaric tags for relative and absolute quantitation; motor nerve; proteomics; sensory nerve; spinal nerve; Wallerian degeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2020;volume=15;issue=2;spage=315;epage=323;aulast=Wei |
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