Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism
In recent years, the development of safe and effective anti-osteoporosis factors has attracted extensive attention. In this study, an estrogen-deficient osteoporosis rat model was employed to study the improving mechanism of sialoglycoprotein isolated from Gadus morhua eggs (Gds) against osteoporosi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.871521/full |
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| author | Meihui Zhao Meihui Zhao Meihui Zhao Fengfeng Mei Fengfeng Mei Fengfeng Mei Jinfeng Lu Qingying Xiang Guanghua Xia Guanghua Xia Guanghua Xia Xueying Zhang Xueying Zhang Xueying Zhang Zhongyuan Liu Zhongyuan Liu Zhongyuan Liu Chenghui Zhang Chenghui Zhang Xuanri Shen Xuanri Shen Xuanri Shen Qiuping Zhong Qiuping Zhong |
| author_facet | Meihui Zhao Meihui Zhao Meihui Zhao Fengfeng Mei Fengfeng Mei Fengfeng Mei Jinfeng Lu Qingying Xiang Guanghua Xia Guanghua Xia Guanghua Xia Xueying Zhang Xueying Zhang Xueying Zhang Zhongyuan Liu Zhongyuan Liu Zhongyuan Liu Chenghui Zhang Chenghui Zhang Xuanri Shen Xuanri Shen Xuanri Shen Qiuping Zhong Qiuping Zhong |
| author_sort | Meihui Zhao |
| collection | DOAJ |
| description | In recent years, the development of safe and effective anti-osteoporosis factors has attracted extensive attention. In this study, an estrogen-deficient osteoporosis rat model was employed to study the improving mechanism of sialoglycoprotein isolated from Gadus morhua eggs (Gds) against osteoporosis. The results showed that compared with OVX, Gds ameliorated the trabecular microstructure, especially the increased trabecular thickness, decreased trabecular separation, and enhanced the trabecular number. The analysis of qRT-PCR and western blotting found that Gds reduced bone resorption by inhibiting RANKL-induced osteoclastogenesis. The LC-MS/MS was used to investigate serum metabolism, and the enrichment metabolites were analyzed by the KEGG pathway. The results revealed that the Gds significantly altered the fat anabolism pathway, which includes ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. Altogether, Gds could improve osteoporosis by suppressing high bone turnover via controlling OPG/RANKL/TRAF6 pathway, which is implicated with ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. These findings indicated that Gds could be a candidate factor for anti-osteoporosis. |
| first_indexed | 2024-04-13T02:57:09Z |
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| id | doaj.art-ce4b41ea34684874a6dba8856beef4b3 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-13T02:57:09Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-ce4b41ea34684874a6dba8856beef4b32022-12-22T03:05:35ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-04-01910.3389/fnut.2022.871521871521Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum MetabolismMeihui Zhao0Meihui Zhao1Meihui Zhao2Fengfeng Mei3Fengfeng Mei4Fengfeng Mei5Jinfeng Lu6Qingying Xiang7Guanghua Xia8Guanghua Xia9Guanghua Xia10Xueying Zhang11Xueying Zhang12Xueying Zhang13Zhongyuan Liu14Zhongyuan Liu15Zhongyuan Liu16Chenghui Zhang17Chenghui Zhang18Xuanri Shen19Xuanri Shen20Xuanri Shen21Qiuping Zhong22Qiuping Zhong23Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaCollaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaCollaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaCollaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaCollaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaCollaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaCollaborative Innovation Center of Provincial and Ministerial Co-construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaHainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, School of Food Science and Engineering, Hainan University, Hainan, ChinaKey Laboratory of Food Nutrition and Functional Food of Hainan Province, Hainan University, Haikou, ChinaIn recent years, the development of safe and effective anti-osteoporosis factors has attracted extensive attention. In this study, an estrogen-deficient osteoporosis rat model was employed to study the improving mechanism of sialoglycoprotein isolated from Gadus morhua eggs (Gds) against osteoporosis. The results showed that compared with OVX, Gds ameliorated the trabecular microstructure, especially the increased trabecular thickness, decreased trabecular separation, and enhanced the trabecular number. The analysis of qRT-PCR and western blotting found that Gds reduced bone resorption by inhibiting RANKL-induced osteoclastogenesis. The LC-MS/MS was used to investigate serum metabolism, and the enrichment metabolites were analyzed by the KEGG pathway. The results revealed that the Gds significantly altered the fat anabolism pathway, which includes ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. Altogether, Gds could improve osteoporosis by suppressing high bone turnover via controlling OPG/RANKL/TRAF6 pathway, which is implicated with ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. These findings indicated that Gds could be a candidate factor for anti-osteoporosis.https://www.frontiersin.org/articles/10.3389/fnut.2022.871521/fullGadus morhuabone resorptionserum metabolismosteoporosisOPG |
| spellingShingle | Meihui Zhao Meihui Zhao Meihui Zhao Fengfeng Mei Fengfeng Mei Fengfeng Mei Jinfeng Lu Qingying Xiang Guanghua Xia Guanghua Xia Guanghua Xia Xueying Zhang Xueying Zhang Xueying Zhang Zhongyuan Liu Zhongyuan Liu Zhongyuan Liu Chenghui Zhang Chenghui Zhang Xuanri Shen Xuanri Shen Xuanri Shen Qiuping Zhong Qiuping Zhong Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism Frontiers in Nutrition Gadus morhua bone resorption serum metabolism osteoporosis OPG |
| title | Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
| title_full | Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
| title_fullStr | Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
| title_full_unstemmed | Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
| title_short | Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism |
| title_sort | gadus morhua eggs sialoglycoprotein prevent estrogen deficiency induced high bone turnover by controlling opg rankl traf6 pathway and serum metabolism |
| topic | Gadus morhua bone resorption serum metabolism osteoporosis OPG |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.871521/full |
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