Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imaging
Two-photon fluorescent trackers for monitoring of lipid droplets (LDs) would be highly effective for illustrating the critical roles of LDs in live cells or tissues. Although a number of one-photon fluorescent trackers for labeling LDs have been developed, their usability remains constrained in live...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2022-11-01
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Series: | Frontiers in Chemistry |
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Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.1072143/full |
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author | Dong Joon Lee Eun Seo Kim Hyo Won Lee Hyo Won Lee Hwan Myung Kim Hwan Myung Kim |
author_facet | Dong Joon Lee Eun Seo Kim Hyo Won Lee Hyo Won Lee Hwan Myung Kim Hwan Myung Kim |
author_sort | Dong Joon Lee |
collection | DOAJ |
description | Two-photon fluorescent trackers for monitoring of lipid droplets (LDs) would be highly effective for illustrating the critical roles of LDs in live cells or tissues. Although a number of one-photon fluorescent trackers for labeling LDs have been developed, their usability remains constrained in live sample imaging due to photo damage, shallow imaging depth, and auto-fluorescence. Recently, some two-photon fluorescent trackers for LDs have been developed to overcome these limitations. In this mini-review article, the advances in two-photon fluorescent trackers for monitoring of LDs are summarized. We summarize the chemical structures, two-photon properties, live sample imaging, and biomedical applications of the most recent representative two-photon fluorescent trackers for LDs. Additionally, the current challenges and future research trends for the two-photon fluorescent trackers of LDs are discussed. |
first_indexed | 2024-04-13T08:06:10Z |
format | Article |
id | doaj.art-ce4ecb65fcb94f8ebc0edfe4fc37162b |
institution | Directory Open Access Journal |
issn | 2296-2646 |
language | English |
last_indexed | 2024-04-13T08:06:10Z |
publishDate | 2022-11-01 |
publisher | Frontiers Media S.A. |
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series | Frontiers in Chemistry |
spelling | doaj.art-ce4ecb65fcb94f8ebc0edfe4fc37162b2022-12-22T02:55:09ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-11-011010.3389/fchem.2022.10721431072143Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imagingDong Joon Lee0Eun Seo Kim1Hyo Won Lee2Hyo Won Lee3Hwan Myung Kim4Hwan Myung Kim5Department of Energy Systems Research, Ajou University, Suwon, South KoreaDepartment of Energy Systems Research, Ajou University, Suwon, South KoreaResearch Institute of Basic Sciences, Suwon, South KoreaDepartment of Chemistry, Ajou University, Suwon, South KoreaDepartment of Energy Systems Research, Ajou University, Suwon, South KoreaDepartment of Chemistry, Ajou University, Suwon, South KoreaTwo-photon fluorescent trackers for monitoring of lipid droplets (LDs) would be highly effective for illustrating the critical roles of LDs in live cells or tissues. Although a number of one-photon fluorescent trackers for labeling LDs have been developed, their usability remains constrained in live sample imaging due to photo damage, shallow imaging depth, and auto-fluorescence. Recently, some two-photon fluorescent trackers for LDs have been developed to overcome these limitations. In this mini-review article, the advances in two-photon fluorescent trackers for monitoring of LDs are summarized. We summarize the chemical structures, two-photon properties, live sample imaging, and biomedical applications of the most recent representative two-photon fluorescent trackers for LDs. Additionally, the current challenges and future research trends for the two-photon fluorescent trackers of LDs are discussed.https://www.frontiersin.org/articles/10.3389/fchem.2022.1072143/fulllipid dropletlipid metabolismorganelle trackertwo-photon microscopylive sample imaging |
spellingShingle | Dong Joon Lee Eun Seo Kim Hyo Won Lee Hyo Won Lee Hwan Myung Kim Hwan Myung Kim Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imaging Frontiers in Chemistry lipid droplet lipid metabolism organelle tracker two-photon microscopy live sample imaging |
title | Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imaging |
title_full | Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imaging |
title_fullStr | Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imaging |
title_full_unstemmed | Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imaging |
title_short | Advances in small molecule two-photon fluorescent trackers for lipid droplets in live sample imaging |
title_sort | advances in small molecule two photon fluorescent trackers for lipid droplets in live sample imaging |
topic | lipid droplet lipid metabolism organelle tracker two-photon microscopy live sample imaging |
url | https://www.frontiersin.org/articles/10.3389/fchem.2022.1072143/full |
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