Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process
Crustacean aquaculture is expected to be a major source of fishery commodities in the near future. Hemocytes are key players of the immune system in shrimps; however, their classification, maturation, and differentiation are still under debate. To date, only discrete and inconsistent information on...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/66954 |
| _version_ | 1811228016296067072 |
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| author | Keiichiro Koiwai Takashi Koyama Soichiro Tsuda Atsushi Toyoda Kiyoshi Kikuchi Hiroaki Suzuki Ryuji Kawano |
| author_facet | Keiichiro Koiwai Takashi Koyama Soichiro Tsuda Atsushi Toyoda Kiyoshi Kikuchi Hiroaki Suzuki Ryuji Kawano |
| author_sort | Keiichiro Koiwai |
| collection | DOAJ |
| description | Crustacean aquaculture is expected to be a major source of fishery commodities in the near future. Hemocytes are key players of the immune system in shrimps; however, their classification, maturation, and differentiation are still under debate. To date, only discrete and inconsistent information on the classification of shrimp hemocytes has been reported, showing that the morphological characteristics are not sufficient to resolve their actual roles. Our present study using single-cell RNA sequencing revealed six types of hemocytes of Marsupenaeus japonicus based on their transcriptional profiles. We identified markers of each subpopulation and predicted the differentiation pathways involved in their maturation. We also predicted cell growth factors that might play crucial roles in hemocyte differentiation. Different immune roles among these subpopulations were suggested from the analysis of differentially expressed immune-related genes. These results provide a unified classification of shrimp hemocytes, which improves the understanding of its immune system. |
| first_indexed | 2024-04-12T09:51:24Z |
| format | Article |
| id | doaj.art-ce4faa3ee4844aeb8934eb6fbddf6809 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:51:24Z |
| publishDate | 2021-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ce4faa3ee4844aeb8934eb6fbddf68092022-12-22T03:37:49ZengeLife Sciences Publications LtdeLife2050-084X2021-06-011010.7554/eLife.66954Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation processKeiichiro Koiwai0https://orcid.org/0000-0002-8890-8229Takashi Koyama1https://orcid.org/0000-0001-7140-7244Soichiro Tsuda2Atsushi Toyoda3https://orcid.org/0000-0002-0728-7548Kiyoshi Kikuchi4Hiroaki Suzuki5https://orcid.org/0000-0002-8899-0955Ryuji Kawano6https://orcid.org/0000-0001-6523-0649Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, Japan; Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Minato, JapanFisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Hamamatsu, Japan; Graduate School of Fisheries and Environmental Sciences, Nagasaki University, Nagasaki, JapanbitBiome Inc, Shinjuku, JapanAdvanced Genomics Center, National Institute of Genetics, Mishima, JapanFisheries Laboratory, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Hamamatsu, JapanDepartment of Precision Mechanics, Faculty of Science and Engineering, Chuo University, Bunkyo, JapanDepartment of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Koganei, JapanCrustacean aquaculture is expected to be a major source of fishery commodities in the near future. Hemocytes are key players of the immune system in shrimps; however, their classification, maturation, and differentiation are still under debate. To date, only discrete and inconsistent information on the classification of shrimp hemocytes has been reported, showing that the morphological characteristics are not sufficient to resolve their actual roles. Our present study using single-cell RNA sequencing revealed six types of hemocytes of Marsupenaeus japonicus based on their transcriptional profiles. We identified markers of each subpopulation and predicted the differentiation pathways involved in their maturation. We also predicted cell growth factors that might play crucial roles in hemocyte differentiation. Different immune roles among these subpopulations were suggested from the analysis of differentially expressed immune-related genes. These results provide a unified classification of shrimp hemocytes, which improves the understanding of its immune system.https://elifesciences.org/articles/66954scRNA-seqnon-model organismshemocytescell differentiationcrustaceanmarsupenaeus japonicus |
| spellingShingle | Keiichiro Koiwai Takashi Koyama Soichiro Tsuda Atsushi Toyoda Kiyoshi Kikuchi Hiroaki Suzuki Ryuji Kawano Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process eLife scRNA-seq non-model organisms hemocytes cell differentiation crustacean marsupenaeus japonicus |
| title | Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process |
| title_full | Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process |
| title_fullStr | Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process |
| title_full_unstemmed | Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process |
| title_short | Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process |
| title_sort | single cell rna seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process |
| topic | scRNA-seq non-model organisms hemocytes cell differentiation crustacean marsupenaeus japonicus |
| url | https://elifesciences.org/articles/66954 |
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