Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination
The human ileostomy model, widely considered the benchmark for determining in vivo starch digestibility, has disadvantages. The ileorectostomised rat model (IRM) is a possible surrogate but evidence as to its validity is scant. In this preliminary study, the resistant starch (RS) content of test bre...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-12-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/13/1/91 |
| _version_ | 1797543188073807872 |
|---|---|
| author | Damien P. Belobrajdic Anthony R. Bird |
| author_facet | Damien P. Belobrajdic Anthony R. Bird |
| author_sort | Damien P. Belobrajdic |
| collection | DOAJ |
| description | The human ileostomy model, widely considered the benchmark for determining in vivo starch digestibility, has disadvantages. The ileorectostomised rat model (IRM) is a possible surrogate but evidence as to its validity is scant. In this preliminary study, the resistant starch (RS) content of test breads made from refined low (LAW-R) and high amylose wheat (HAW-R) flours was established in a randomised cross-over trial involving six human ileostomy participants. Starch digestibility of refined breads and diets made from these flours was then evaluated in ileorectostomised rats using a similar experimental format. Physical performance measures and other data were also collected for the rat model. The amount of RS in the low- and high-amylose breads as measured using the human model was 0.8 ± 0.1 and 6.5 ± 0.3 g/100 g, respectively. The RS level of HAW-R bread determined using ileorectostomised rats was 5.5 ± 0.8 g/100 g, about 15% less than that recorded in the human study, whereas for conventional wheat breads the models produced similar RS values. While offering promise, further validation using a wide variety of starchy food products is needed before the IRM can be considered an acceptable alternative for RS determination. |
| first_indexed | 2024-03-10T13:41:02Z |
| format | Article |
| id | doaj.art-ce5390f78e76481f8a2249dff7ead585 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T13:41:02Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-ce5390f78e76481f8a2249dff7ead5852023-11-21T03:03:54ZengMDPI AGNutrients2072-66432020-12-011319110.3390/nu13010091Evaluation of an Ileorectostomised Rat Model for Resistant Starch DeterminationDamien P. Belobrajdic0Anthony R. Bird1CSIRO Health and Biosecurity, Adelaide, SA 5000, AustraliaCSIRO Health and Biosecurity, Adelaide, SA 5000, AustraliaThe human ileostomy model, widely considered the benchmark for determining in vivo starch digestibility, has disadvantages. The ileorectostomised rat model (IRM) is a possible surrogate but evidence as to its validity is scant. In this preliminary study, the resistant starch (RS) content of test breads made from refined low (LAW-R) and high amylose wheat (HAW-R) flours was established in a randomised cross-over trial involving six human ileostomy participants. Starch digestibility of refined breads and diets made from these flours was then evaluated in ileorectostomised rats using a similar experimental format. Physical performance measures and other data were also collected for the rat model. The amount of RS in the low- and high-amylose breads as measured using the human model was 0.8 ± 0.1 and 6.5 ± 0.3 g/100 g, respectively. The RS level of HAW-R bread determined using ileorectostomised rats was 5.5 ± 0.8 g/100 g, about 15% less than that recorded in the human study, whereas for conventional wheat breads the models produced similar RS values. While offering promise, further validation using a wide variety of starchy food products is needed before the IRM can be considered an acceptable alternative for RS determination.https://www.mdpi.com/2072-6643/13/1/91ileostomyhigh amylose wheatresistant starchrat |
| spellingShingle | Damien P. Belobrajdic Anthony R. Bird Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination Nutrients ileostomy high amylose wheat resistant starch rat |
| title | Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination |
| title_full | Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination |
| title_fullStr | Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination |
| title_full_unstemmed | Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination |
| title_short | Evaluation of an Ileorectostomised Rat Model for Resistant Starch Determination |
| title_sort | evaluation of an ileorectostomised rat model for resistant starch determination |
| topic | ileostomy high amylose wheat resistant starch rat |
| url | https://www.mdpi.com/2072-6643/13/1/91 |
| work_keys_str_mv | AT damienpbelobrajdic evaluationofanileorectostomisedratmodelforresistantstarchdetermination AT anthonyrbird evaluationofanileorectostomisedratmodelforresistantstarchdetermination |