Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods
Introduction: We aimed to explore the abnormal expression of dual-specificity protein phosphatase 1 (DUSP1) and its latent molecular mechanisms in ovarian carcinoma (OVCA).Materials and Methods: Two clinical cohorts collected from two different hospitals were used to evaluate the expression of DUSP1...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Pathology and Oncology Research |
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| Online Access: | https://www.por-journal.com/articles/10.3389/pore.2022.1610404/full |
| _version_ | 1797221104313434112 |
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| author | Zi-Qian Liang Rong-Quan He Jia-Yuan Luo Zhi-Guang Huang Jie Li Lu-Yang Zhong Jun-Hong Chen Su-Ning Huang Lin Shi Kang-Lai Wei Jiang-Hui Zeng Jing-Jing Zeng Gang Chen |
| author_facet | Zi-Qian Liang Rong-Quan He Jia-Yuan Luo Zhi-Guang Huang Jie Li Lu-Yang Zhong Jun-Hong Chen Su-Ning Huang Lin Shi Kang-Lai Wei Jiang-Hui Zeng Jing-Jing Zeng Gang Chen |
| author_sort | Zi-Qian Liang |
| collection | DOAJ |
| description | Introduction: We aimed to explore the abnormal expression of dual-specificity protein phosphatase 1 (DUSP1) and its latent molecular mechanisms in ovarian carcinoma (OVCA).Materials and Methods: Two clinical cohorts collected from two different hospitals were used to evaluate the expression of DUSP1 protein in OVCA tissues. RNA-sequencing and microarray datasets were utilised to verify DUSP1 expression at mRNA levels in both OVCA tissues and in the peripheral blood of OVCA patients. Furthermore, an integrated calculation was performed to pool the standard mean difference (SMD) from each cohort in order to comprehensively assess the expression of DUSP1 in OVCA. Furthermore, we examined the relationship among DUSP1, tumour microenvironment (TME), and chemotherapy resistance in OVCA. Moreover, we used pathway enrichment analysis to explore the underlying mechanisms of DUSP1 in OVCA.Results: A pooled SMD of −1.19 (95% CI [−2.00, −0.38], p = 0.004) with 1,240 samples revealed that DUSP1 was downregulated in OVCA at both mRNA and protein levels. The area under the receiver operating characteristic curve of 0.9235 indicated the downregulated DUSP1 in peripheral blood may have a non-invasive diagnostic value in OVCA. Through six algorithms, we identified that DUSP1 may related to tumour-infiltrating T cells and cancer associated fibroblasts (CAFs) in OVCA. Pathway enrichment demonstrated that DUSP1 might participate in the mitogen-activated protein kinase (MAPK) signalling pathway. Furthermore, DUSP1 may have relations with chemotherapy resistance, and a favourable combining affinity was observed in the paclitaxel-DUSP1 docking model.Conclusion: DUSP1 was downregulated in OVCA, and this decreasing trend may affect the infiltration of CAFs. Finally, DUSP1 may have a targeting relation with paclitaxel and participate in MAPK signaling pathways. |
| first_indexed | 2024-04-24T13:00:08Z |
| format | Article |
| id | doaj.art-ce5440f52b5d41b798626bdf9b8e6690 |
| institution | Directory Open Access Journal |
| issn | 1532-2807 |
| language | English |
| last_indexed | 2024-04-24T13:00:08Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Pathology and Oncology Research |
| spelling | doaj.art-ce5440f52b5d41b798626bdf9b8e66902024-04-05T16:19:55ZengFrontiers Media S.A.Pathology and Oncology Research1532-28072022-07-012810.3389/pore.2022.16104041610404Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple MethodsZi-Qian Liang0Rong-Quan He1Jia-Yuan Luo2Zhi-Guang Huang3Jie Li4Lu-Yang Zhong5Jun-Hong Chen6Su-Ning Huang7Lin Shi8Kang-Lai Wei9Jiang-Hui Zeng10Jing-Jing Zeng11Gang Chen12Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Medical Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pathology, Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region, Nanning, ChinaDepartment of Radiotherapy, Guangxi Medical University Cancer Hospital, Nanning, ChinaDepartment of Pathology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pathology, The Second Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Clinical Laboratory, The Third Affiliated Hospital of Guangxi Medical University/Nanning Second People’s Hospital, Nanning, ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, ChinaIntroduction: We aimed to explore the abnormal expression of dual-specificity protein phosphatase 1 (DUSP1) and its latent molecular mechanisms in ovarian carcinoma (OVCA).Materials and Methods: Two clinical cohorts collected from two different hospitals were used to evaluate the expression of DUSP1 protein in OVCA tissues. RNA-sequencing and microarray datasets were utilised to verify DUSP1 expression at mRNA levels in both OVCA tissues and in the peripheral blood of OVCA patients. Furthermore, an integrated calculation was performed to pool the standard mean difference (SMD) from each cohort in order to comprehensively assess the expression of DUSP1 in OVCA. Furthermore, we examined the relationship among DUSP1, tumour microenvironment (TME), and chemotherapy resistance in OVCA. Moreover, we used pathway enrichment analysis to explore the underlying mechanisms of DUSP1 in OVCA.Results: A pooled SMD of −1.19 (95% CI [−2.00, −0.38], p = 0.004) with 1,240 samples revealed that DUSP1 was downregulated in OVCA at both mRNA and protein levels. The area under the receiver operating characteristic curve of 0.9235 indicated the downregulated DUSP1 in peripheral blood may have a non-invasive diagnostic value in OVCA. Through six algorithms, we identified that DUSP1 may related to tumour-infiltrating T cells and cancer associated fibroblasts (CAFs) in OVCA. Pathway enrichment demonstrated that DUSP1 might participate in the mitogen-activated protein kinase (MAPK) signalling pathway. Furthermore, DUSP1 may have relations with chemotherapy resistance, and a favourable combining affinity was observed in the paclitaxel-DUSP1 docking model.Conclusion: DUSP1 was downregulated in OVCA, and this decreasing trend may affect the infiltration of CAFs. Finally, DUSP1 may have a targeting relation with paclitaxel and participate in MAPK signaling pathways.https://www.por-journal.com/articles/10.3389/pore.2022.1610404/fullmolecular dockingdual-specificity protein phosphatase 1immune landscapecancer associated fibroblastsovarian carcinoma |
| spellingShingle | Zi-Qian Liang Rong-Quan He Jia-Yuan Luo Zhi-Guang Huang Jie Li Lu-Yang Zhong Jun-Hong Chen Su-Ning Huang Lin Shi Kang-Lai Wei Jiang-Hui Zeng Jing-Jing Zeng Gang Chen Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods Pathology and Oncology Research molecular docking dual-specificity protein phosphatase 1 immune landscape cancer associated fibroblasts ovarian carcinoma |
| title | Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods |
| title_full | Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods |
| title_fullStr | Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods |
| title_full_unstemmed | Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods |
| title_short | Downregulated Dual-Specificity Protein Phosphatase 1 in Ovarian Carcinoma: A Comprehensive Study With Multiple Methods |
| title_sort | downregulated dual specificity protein phosphatase 1 in ovarian carcinoma a comprehensive study with multiple methods |
| topic | molecular docking dual-specificity protein phosphatase 1 immune landscape cancer associated fibroblasts ovarian carcinoma |
| url | https://www.por-journal.com/articles/10.3389/pore.2022.1610404/full |
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