Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section
Introduction: Estrogen Receptor (ER), Progesterone Receptor (PR) and Her2/neu are routinely studied markers for breast carcinoma. Analysis of these biomarkers is traditionally done by Immunohistochemistry (IHC) on whole sections. These markers can also be studied on Tissue Microarray (TMA) secti...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-03-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9573/25088_CE[Ra1]_F(DK)_PF1(SY_RK)_PFA(RK)_PF2(P_NESY).pdf |
| Summary: | Introduction: Estrogen Receptor (ER), Progesterone Receptor
(PR) and Her2/neu are routinely studied markers for breast
carcinoma. Analysis of these biomarkers is traditionally done by
Immunohistochemistry (IHC) on whole sections. These markers
can also be studied on Tissue Microarray (TMA) sections. Tissue
microarray is a technique where core samples from different
paraffin blocks are arrayed on a single recipient block which can
then be cut to yield a single section with multiple cores in it.
Aim: To compare ER, PR and Her2/neu on TMA sections with
whole sections and to determine the concordance of results
between the two methods.
Materials and Methods: A TMA block was constructed by
punching out 2 mm cores from appropriately marked paraffin
blocks of 53 breast carcinoma cases and embedding them in
the recipient block. Immunostaining of TMA sections and whole
sections were performed for ER, PR and Her2/neu and the results
were compared.
Statistical analysis was done using chi square test/FisherExact test. Kappa co-efficient, Jaccard Index and G-Index were
computed.
Results: Infiltrating Ductal Carcinoma-No Special Type (IDCNST) was the predominant type of carcinoma and most of the
tumours were of Grade II and III. Majority, 38/53 (71.7%) were
ER/PR positive and Her2 negative and 9/53 (17%) cases were
triple negative. Good concordance between whole sections
and TMA sections were noted with kappa value for ER, PR and
Her2/neu being 0.671, 0.754, 1.000 respectively which was
statistically significant.
Conclusion: Immunostaining for ER, PR and Her2/neu done
on TMA section using single 2 mm core were comparable with
conventional whole section scores. Thus, TMA is a reliable
method for evaluating these biomarkers with the advantage of
being time and cost effective. |
|---|---|
| ISSN: | 2249-782X 0973-709X |