Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section
Introduction: Estrogen Receptor (ER), Progesterone Receptor (PR) and Her2/neu are routinely studied markers for breast carcinoma. Analysis of these biomarkers is traditionally done by Immunohistochemistry (IHC) on whole sections. These markers can also be studied on Tissue Microarray (TMA) secti...
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9573/25088_CE[Ra1]_F(DK)_PF1(SY_RK)_PFA(RK)_PF2(P_NESY).pdf |
| _version_ | 1819016495771942912 |
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| author | Sneha S Chavan Savithri Ravindra MSN Prasad |
| author_facet | Sneha S Chavan Savithri Ravindra MSN Prasad |
| author_sort | Sneha S Chavan |
| collection | DOAJ |
| description | Introduction: Estrogen Receptor (ER), Progesterone Receptor
(PR) and Her2/neu are routinely studied markers for breast
carcinoma. Analysis of these biomarkers is traditionally done by
Immunohistochemistry (IHC) on whole sections. These markers
can also be studied on Tissue Microarray (TMA) sections. Tissue
microarray is a technique where core samples from different
paraffin blocks are arrayed on a single recipient block which can
then be cut to yield a single section with multiple cores in it.
Aim: To compare ER, PR and Her2/neu on TMA sections with
whole sections and to determine the concordance of results
between the two methods.
Materials and Methods: A TMA block was constructed by
punching out 2 mm cores from appropriately marked paraffin
blocks of 53 breast carcinoma cases and embedding them in
the recipient block. Immunostaining of TMA sections and whole
sections were performed for ER, PR and Her2/neu and the results
were compared.
Statistical analysis was done using chi square test/FisherExact test. Kappa co-efficient, Jaccard Index and G-Index were
computed.
Results: Infiltrating Ductal Carcinoma-No Special Type (IDCNST) was the predominant type of carcinoma and most of the
tumours were of Grade II and III. Majority, 38/53 (71.7%) were
ER/PR positive and Her2 negative and 9/53 (17%) cases were
triple negative. Good concordance between whole sections
and TMA sections were noted with kappa value for ER, PR and
Her2/neu being 0.671, 0.754, 1.000 respectively which was
statistically significant.
Conclusion: Immunostaining for ER, PR and Her2/neu done
on TMA section using single 2 mm core were comparable with
conventional whole section scores. Thus, TMA is a reliable
method for evaluating these biomarkers with the advantage of
being time and cost effective. |
| first_indexed | 2024-12-21T02:48:33Z |
| format | Article |
| id | doaj.art-ce576b0d4789417aa08b22d778ff7467 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-21T02:48:33Z |
| publishDate | 2017-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-ce576b0d4789417aa08b22d778ff74672022-12-21T19:18:30ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-03-01113EC40EC4410.7860/JCDR/2017/25088.9573Breast Biomarkers-Comparison on Whole Section and Tissue Microarray SectionSneha S Chavan0Savithri Ravindra1MSN Prasad2Postgraduate Student, Department of Pathology, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India.Associate Professor, Department of Pathology, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India.Consultant Pathologist, Department of Pathology, Sri Shankara Cancer Hospital and Research Centre, Bengaluru, Karnataka, India.Introduction: Estrogen Receptor (ER), Progesterone Receptor (PR) and Her2/neu are routinely studied markers for breast carcinoma. Analysis of these biomarkers is traditionally done by Immunohistochemistry (IHC) on whole sections. These markers can also be studied on Tissue Microarray (TMA) sections. Tissue microarray is a technique where core samples from different paraffin blocks are arrayed on a single recipient block which can then be cut to yield a single section with multiple cores in it. Aim: To compare ER, PR and Her2/neu on TMA sections with whole sections and to determine the concordance of results between the two methods. Materials and Methods: A TMA block was constructed by punching out 2 mm cores from appropriately marked paraffin blocks of 53 breast carcinoma cases and embedding them in the recipient block. Immunostaining of TMA sections and whole sections were performed for ER, PR and Her2/neu and the results were compared. Statistical analysis was done using chi square test/FisherExact test. Kappa co-efficient, Jaccard Index and G-Index were computed. Results: Infiltrating Ductal Carcinoma-No Special Type (IDCNST) was the predominant type of carcinoma and most of the tumours were of Grade II and III. Majority, 38/53 (71.7%) were ER/PR positive and Her2 negative and 9/53 (17%) cases were triple negative. Good concordance between whole sections and TMA sections were noted with kappa value for ER, PR and Her2/neu being 0.671, 0.754, 1.000 respectively which was statistically significant. Conclusion: Immunostaining for ER, PR and Her2/neu done on TMA section using single 2 mm core were comparable with conventional whole section scores. Thus, TMA is a reliable method for evaluating these biomarkers with the advantage of being time and cost effective.https://jcdr.net/articles/PDF/9573/25088_CE[Ra1]_F(DK)_PF1(SY_RK)_PFA(RK)_PF2(P_NESY).pdfbreast carcinomaestrogen receptorher2/neu |
| spellingShingle | Sneha S Chavan Savithri Ravindra MSN Prasad Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section Journal of Clinical and Diagnostic Research breast carcinoma estrogen receptor her2/neu |
| title | Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section |
| title_full | Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section |
| title_fullStr | Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section |
| title_full_unstemmed | Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section |
| title_short | Breast Biomarkers-Comparison on Whole Section and Tissue Microarray Section |
| title_sort | breast biomarkers comparison on whole section and tissue microarray section |
| topic | breast carcinoma estrogen receptor her2/neu |
| url | https://jcdr.net/articles/PDF/9573/25088_CE[Ra1]_F(DK)_PF1(SY_RK)_PFA(RK)_PF2(P_NESY).pdf |
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