Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells
Summary: In vivo and in vitro systems have the potential to provide a framework to study the organization, gene expression, and functionality of lymphatic and blood vessel smooth muscle cells in physiology and disease settings. A series of procedures are described here, including the surgical isolat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006803 |
| _version_ | 1828116897006092288 |
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| author | Guillermo Arroyo-Ataz Dennis Jones |
| author_facet | Guillermo Arroyo-Ataz Dennis Jones |
| author_sort | Guillermo Arroyo-Ataz |
| collection | DOAJ |
| description | Summary: In vivo and in vitro systems have the potential to provide a framework to study the organization, gene expression, and functionality of lymphatic and blood vessel smooth muscle cells in physiology and disease settings. A series of procedures are described here, including the surgical isolation of mouse collecting lymphatic vessels and blood vessels, whole-mount immunofluorescence staining of muscle cells on the vessels, and the enzymatic digestion and culture of α- smooth muscle actin+ cells from the vessels.For complete details on the use and execution of this protocol, please refer to Jones et al. (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T13:04:14Z |
| format | Article |
| id | doaj.art-ce593aec658b400a9cb8549eeb874206 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T13:04:14Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-ce593aec658b400a9cb8549eeb8742062022-12-22T04:22:50ZengElsevierSTAR Protocols2666-16672022-12-0134101800Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cellsGuillermo Arroyo-Ataz0Dennis Jones1Department of Pathology and Laboratory Medicine, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; Corresponding authorDepartment of Pathology and Laboratory Medicine, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA; Corresponding authorSummary: In vivo and in vitro systems have the potential to provide a framework to study the organization, gene expression, and functionality of lymphatic and blood vessel smooth muscle cells in physiology and disease settings. A series of procedures are described here, including the surgical isolation of mouse collecting lymphatic vessels and blood vessels, whole-mount immunofluorescence staining of muscle cells on the vessels, and the enzymatic digestion and culture of α- smooth muscle actin+ cells from the vessels.For complete details on the use and execution of this protocol, please refer to Jones et al. (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006803Cell cultureCell isolationSingle cellDevelopmental biologyMicroscopyStem cells |
| spellingShingle | Guillermo Arroyo-Ataz Dennis Jones Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells STAR Protocols Cell culture Cell isolation Single cell Developmental biology Microscopy Stem cells |
| title | Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells |
| title_full | Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells |
| title_fullStr | Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells |
| title_full_unstemmed | Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells |
| title_short | Dissection of murine collecting lymphatic vessels for imaging, single-cell analysis, and tissue culture of lymphatic muscle cells |
| title_sort | dissection of murine collecting lymphatic vessels for imaging single cell analysis and tissue culture of lymphatic muscle cells |
| topic | Cell culture Cell isolation Single cell Developmental biology Microscopy Stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006803 |
| work_keys_str_mv | AT guillermoarroyoataz dissectionofmurinecollectinglymphaticvesselsforimagingsinglecellanalysisandtissuecultureoflymphaticmusclecells AT dennisjones dissectionofmurinecollectinglymphaticvesselsforimagingsinglecellanalysisandtissuecultureoflymphaticmusclecells |