Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus
In the present study, Monascus red biopigment produced by solid-state fermentation was evaluated for its anti-microbial and antioxidative potential. The antibacterial activity through Scanning Electron Microscopy against Bacillus cereus, Escherichia coli, and Staphylococcus aureus was found to show...
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Action for Sustainable Efficacious Development and Awareness
2022-02-01
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| Series: | Environment Conservation Journal |
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| Online Access: | https://journal.environcj.in/index.php/ecj/article/view/833 |
| _version_ | 1811240485752143872 |
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| author | Vishu Chaudhary Priya Katyal Harsh Panwar Anil Kumar Puniya Anuj Kumar Poonia |
| author_facet | Vishu Chaudhary Priya Katyal Harsh Panwar Anil Kumar Puniya Anuj Kumar Poonia |
| author_sort | Vishu Chaudhary |
| collection | DOAJ |
| description |
In the present study, Monascus red biopigment produced by solid-state fermentation was evaluated for its anti-microbial and antioxidative potential. The antibacterial activity through Scanning Electron Microscopy against Bacillus cereus, Escherichia coli, and Staphylococcus aureus was found to show morphological damage in some cells, as evidenced by the outflow of cell contents, deep craters, burst cells, and cell death at concentration of 200 µg/ml of biopigment. Moreover, antibacterial activity through agar well diffusion method against Bacillus cereus, Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa was in range of 2-6 mm by varying concentration of biopigment from 1 to 20 mg/ml.Next, the antifungal activity of the extracted biopigment was in the range of 2-9 mm for Aspergillus flavus, Fusarium oxysporum and Alternaria alternata. In addition, antioxidant efficacy of red biopigment through DPPH, ABTS and FRAP assay results was found to show 59.69 %, 91.1 %, and 15.22 % free radical scavenging activity. The results of this study revealed that red biopigment has potential to modulate the antimicrobial and antioxidative activity.
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| first_indexed | 2024-04-12T13:21:04Z |
| format | Article |
| id | doaj.art-ce624b380df946248dd1afe78dc6099c |
| institution | Directory Open Access Journal |
| issn | 0972-3099 2278-5124 |
| language | English |
| last_indexed | 2024-04-12T13:21:04Z |
| publishDate | 2022-02-01 |
| publisher | Action for Sustainable Efficacious Development and Awareness |
| record_format | Article |
| series | Environment Conservation Journal |
| spelling | doaj.art-ce624b380df946248dd1afe78dc6099c2022-12-22T03:31:29ZengAction for Sustainable Efficacious Development and AwarenessEnvironment Conservation Journal0972-30992278-51242022-02-01231&210.36953/ECJ.021833-2131Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureusVishu Chaudhary0Priya Katyal1Harsh Panwar2Anil Kumar Puniya3Anuj Kumar Poonia4Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana, Punjab, IndiaDepartment of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana, Punjab, India.Department of Dairy Microbiology, College of Dairy Science and Technology, Guru Angad Dev Veterinary and Animal Sciences University Ludhiana-Punjab, IndiaDepartment of Dairy Microbiology, ICAR-National Dairy Research Institute, Karnal, Haryana, IndiaDepartment of Applied Sciences and Biotechnology, School of Biotechnology, Shoolini University, Solan, Himachal Pradesh, India In the present study, Monascus red biopigment produced by solid-state fermentation was evaluated for its anti-microbial and antioxidative potential. The antibacterial activity through Scanning Electron Microscopy against Bacillus cereus, Escherichia coli, and Staphylococcus aureus was found to show morphological damage in some cells, as evidenced by the outflow of cell contents, deep craters, burst cells, and cell death at concentration of 200 µg/ml of biopigment. Moreover, antibacterial activity through agar well diffusion method against Bacillus cereus, Staphylococcus aureus, Klebsiella pneumonia and Pseudomonas aeruginosa was in range of 2-6 mm by varying concentration of biopigment from 1 to 20 mg/ml.Next, the antifungal activity of the extracted biopigment was in the range of 2-9 mm for Aspergillus flavus, Fusarium oxysporum and Alternaria alternata. In addition, antioxidant efficacy of red biopigment through DPPH, ABTS and FRAP assay results was found to show 59.69 %, 91.1 %, and 15.22 % free radical scavenging activity. The results of this study revealed that red biopigment has potential to modulate the antimicrobial and antioxidative activity. https://journal.environcj.in/index.php/ecj/article/view/833AntimicrobialAntioxidativeFood colorantsMonascus purpureusRed Biopigment |
| spellingShingle | Vishu Chaudhary Priya Katyal Harsh Panwar Anil Kumar Puniya Anuj Kumar Poonia Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus Environment Conservation Journal Antimicrobial Antioxidative Food colorants Monascus purpureus Red Biopigment |
| title | Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus |
| title_full | Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus |
| title_fullStr | Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus |
| title_full_unstemmed | Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus |
| title_short | Evaluating anti-microbial and anti-oxidative potential of red biopigment from Monascus purpureus |
| title_sort | evaluating anti microbial and anti oxidative potential of red biopigment from monascus purpureus |
| topic | Antimicrobial Antioxidative Food colorants Monascus purpureus Red Biopigment |
| url | https://journal.environcj.in/index.php/ecj/article/view/833 |
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