Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion
BackgroundThe simple, rapid, and accurate diagnosis of tuberculous pleural effusion (TPE) remains difficult. This study aimed to determine the accuracy of hepatocyte growth factor (HGF) in the diagnosis of TPE.MethodsWe quantified the expression of HGF, adenosine deaminase (ADA), and interferon gamm...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-07-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1181912/full |
| _version_ | 1797785682737889280 |
|---|---|
| author | Sheng-Cai Zheng Sheng-Cai Zheng Zhong-Yin Huang Zhong-Yin Huang Kan Zhai Kan Zhai Huan-Zhong Shi Huan-Zhong Shi Ming-Ming Shao Ming-Ming Shao |
| author_facet | Sheng-Cai Zheng Sheng-Cai Zheng Zhong-Yin Huang Zhong-Yin Huang Kan Zhai Kan Zhai Huan-Zhong Shi Huan-Zhong Shi Ming-Ming Shao Ming-Ming Shao |
| author_sort | Sheng-Cai Zheng |
| collection | DOAJ |
| description | BackgroundThe simple, rapid, and accurate diagnosis of tuberculous pleural effusion (TPE) remains difficult. This study aimed to determine the accuracy of hepatocyte growth factor (HGF) in the diagnosis of TPE.MethodsWe quantified the expression of HGF, adenosine deaminase (ADA), and interferon gamma (IFN-γ) in pleural effusion (PE) in 97 TPE subjects and 116 non-TPE subjects using an enzyme-linked immunosorbent assay (ELISA) or a fully automatic biochemical analyzer. The diagnostic performance of these three biomarkers was evaluated using a receiver operating characteristic (ROC) curve of subjects by age and gender.ResultsWe discovered that the TPE group had much higher levels of HGF than the non-TPE group, regardless of age or gender, and that there was no statistically significant difference between the two groups’ levels of HGF expression in peripheral plasma. In female TPE patients aged ≤65 years, the AUCs of TPE and non-TPE diagnosed by HGF, ADA or IFN-γ were 0.988, 0.964, and 0.827, respectively. HGF plus ADA had the highest diagnostic efficacy in female TPE patients aged ≤65 years. With HGF plus ADA having a cut-off value of 0.219 for distinguishing TPE from non-TPE, the area under the curve (AUC), sensitivity (SEN), specificity (SPE), positive predictive value (PPV), and negative predictive value (NPV) were, respectively, 0.998 (95% confidence interval [CI], 0.993–1.000), 100 (95% CI, 89.997–100.000), 96.667 (95% CI, 82.783–99.916), 97.222 (95% CI, 83.594–99.586), and 100.ConclusionThis study confirmed that HGF plus ADA has high diagnostic efficacy in younger female TPE patients and has the potential to be an excellent biomarker. |
| first_indexed | 2024-03-13T00:57:27Z |
| format | Article |
| id | doaj.art-ce66c819eb48419f957c4f0535f8f9ce |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T00:57:27Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ce66c819eb48419f957c4f0535f8f9ce2023-07-06T16:51:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.11819121181912Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusionSheng-Cai Zheng0Sheng-Cai Zheng1Zhong-Yin Huang2Zhong-Yin Huang3Kan Zhai4Kan Zhai5Huan-Zhong Shi6Huan-Zhong Shi7Ming-Ming Shao8Ming-Ming Shao9Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaClinical Center for Pleural Diseases, Capital Medical University, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaClinical Center for Pleural Diseases, Capital Medical University, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaClinical Center for Pleural Diseases, Capital Medical University, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaClinical Center for Pleural Diseases, Capital Medical University, Beijing, ChinaDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaClinical Center for Pleural Diseases, Capital Medical University, Beijing, ChinaBackgroundThe simple, rapid, and accurate diagnosis of tuberculous pleural effusion (TPE) remains difficult. This study aimed to determine the accuracy of hepatocyte growth factor (HGF) in the diagnosis of TPE.MethodsWe quantified the expression of HGF, adenosine deaminase (ADA), and interferon gamma (IFN-γ) in pleural effusion (PE) in 97 TPE subjects and 116 non-TPE subjects using an enzyme-linked immunosorbent assay (ELISA) or a fully automatic biochemical analyzer. The diagnostic performance of these three biomarkers was evaluated using a receiver operating characteristic (ROC) curve of subjects by age and gender.ResultsWe discovered that the TPE group had much higher levels of HGF than the non-TPE group, regardless of age or gender, and that there was no statistically significant difference between the two groups’ levels of HGF expression in peripheral plasma. In female TPE patients aged ≤65 years, the AUCs of TPE and non-TPE diagnosed by HGF, ADA or IFN-γ were 0.988, 0.964, and 0.827, respectively. HGF plus ADA had the highest diagnostic efficacy in female TPE patients aged ≤65 years. With HGF plus ADA having a cut-off value of 0.219 for distinguishing TPE from non-TPE, the area under the curve (AUC), sensitivity (SEN), specificity (SPE), positive predictive value (PPV), and negative predictive value (NPV) were, respectively, 0.998 (95% confidence interval [CI], 0.993–1.000), 100 (95% CI, 89.997–100.000), 96.667 (95% CI, 82.783–99.916), 97.222 (95% CI, 83.594–99.586), and 100.ConclusionThis study confirmed that HGF plus ADA has high diagnostic efficacy in younger female TPE patients and has the potential to be an excellent biomarker.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1181912/fulltuberculous pleural effusionHGFADAdiagnostic efficacybiomarkers |
| spellingShingle | Sheng-Cai Zheng Sheng-Cai Zheng Zhong-Yin Huang Zhong-Yin Huang Kan Zhai Kan Zhai Huan-Zhong Shi Huan-Zhong Shi Ming-Ming Shao Ming-Ming Shao Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion Frontiers in Microbiology tuberculous pleural effusion HGF ADA diagnostic efficacy biomarkers |
| title | Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion |
| title_full | Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion |
| title_fullStr | Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion |
| title_full_unstemmed | Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion |
| title_short | Hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion |
| title_sort | hepatocyte growth factor combined with adenosine deaminase as biomarker for diagnosis of tuberculous pleural effusion |
| topic | tuberculous pleural effusion HGF ADA diagnostic efficacy biomarkers |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1181912/full |
| work_keys_str_mv | AT shengcaizheng hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT shengcaizheng hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT zhongyinhuang hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT zhongyinhuang hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT kanzhai hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT kanzhai hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT huanzhongshi hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT huanzhongshi hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT mingmingshao hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion AT mingmingshao hepatocytegrowthfactorcombinedwithadenosinedeaminaseasbiomarkerfordiagnosisoftuberculouspleuraleffusion |