Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation
Abstract Background Cell therapy is a key technology to prevent sacrificing normal skin. Although some studies have shown the promise of human epidermal stem cells (EpiSCs), the efficacy, biosafety and quality control of EpiSC therapy have not been systematically reported. Methods The biosafety, ste...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-022-03202-6 |
| _version_ | 1797958835536658432 |
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| author | Xiaohong Zhao Xue Li Ying Wang Yicheng Guo Yong Huang Dalun Lv Mingxing Lei Shicang Yu Gaoxing Luo Rixing Zhan |
| author_facet | Xiaohong Zhao Xue Li Ying Wang Yicheng Guo Yong Huang Dalun Lv Mingxing Lei Shicang Yu Gaoxing Luo Rixing Zhan |
| author_sort | Xiaohong Zhao |
| collection | DOAJ |
| description | Abstract Background Cell therapy is a key technology to prevent sacrificing normal skin. Although some studies have shown the promise of human epidermal stem cells (EpiSCs), the efficacy, biosafety and quality control of EpiSC therapy have not been systematically reported. Methods The biosafety, stemness maintenance and wound repair of EpiSC were systematically verified by in vitro and in vivo experiments. EpiSC were prepared from the foreskin using a collagen type IV rapid adherence method. The EpiSCs were identified by flow cytometry, immunofluorescence staining and cell morphology. The well-growing passage 1 (P1) EpiSCs were used to determine the proliferation curve (counting method). EpiSC clone formation assay was performed by Giemsa staining. Nude mice were used to prepare a full-thickness skin defect wound model to detect the repair effect of EpiSCs. The biosafety of EpiSCs was double tested in vitro and in vivo. Results The results showed that the expression of specific markers and clone formation efficiency was stable when passage 1 (P1) to P8 cells were cultured, and the stemness rate of P8 cells was close to 85.1%. EpiSCs were expanded in vitro for 25 days, the number of cells reached 2.5 × 108, and the transplantable area was approximately 75% of the total body surface area (TBSA). At 45 days, the total number of cells was approximately 30 billion, and the transplantable area was approximately the size of a volleyball court. A nude mouse wound model indicated that EpiSCs could rapidly close a wound. On postinjury day 7, the wound epithelialization rate in the cell transplantation group was significantly higher than that in the NaCl group (P < 0.05). In vitro, cell senescence increased, and telomerase activity decreased in P1 to P8 EpiSCs. In vivo, there were no solid tumors or metastatic tumors after EpiSC (P8) transplantation. In addition, the quality control of cultured cells met the clinical application criteria for cell therapy. Conclusion This preclinical study showed the stability and biosafety of human EpiSC therapy for wound repair. Graphical Abstract |
| first_indexed | 2024-04-11T00:24:27Z |
| format | Article |
| id | doaj.art-ce67ee1221a34f3e97033a7527e8426a |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-04-11T00:24:27Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-ce67ee1221a34f3e97033a7527e8426a2023-01-08T12:06:11ZengBMCStem Cell Research & Therapy1757-65122023-01-0114111410.1186/s13287-022-03202-6Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluationXiaohong Zhao0Xue Li1Ying Wang2Yicheng Guo3Yong Huang4Dalun Lv5Mingxing Lei6Shicang Yu7Gaoxing Luo8Rixing Zhan9Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University)Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University)Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University)Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University)Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University)Department of Burn and Plastic Surgery, The First Affiliated Hospital of Wannan Medical College“111” Project Laboratory of Biomechanics and Tissue Repair, College of Bioengineering, Chongqing UniversityStem Cell and Regenerative Medicine, Southwest Hospital, The Third Military Medical University (Army Medical University)Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University)Institute of Burn Research, State Key Laboratory of Trauma, Burn and Combined Injury, Southwest Hospital, The Third Military Medical University (Army Medical University)Abstract Background Cell therapy is a key technology to prevent sacrificing normal skin. Although some studies have shown the promise of human epidermal stem cells (EpiSCs), the efficacy, biosafety and quality control of EpiSC therapy have not been systematically reported. Methods The biosafety, stemness maintenance and wound repair of EpiSC were systematically verified by in vitro and in vivo experiments. EpiSC were prepared from the foreskin using a collagen type IV rapid adherence method. The EpiSCs were identified by flow cytometry, immunofluorescence staining and cell morphology. The well-growing passage 1 (P1) EpiSCs were used to determine the proliferation curve (counting method). EpiSC clone formation assay was performed by Giemsa staining. Nude mice were used to prepare a full-thickness skin defect wound model to detect the repair effect of EpiSCs. The biosafety of EpiSCs was double tested in vitro and in vivo. Results The results showed that the expression of specific markers and clone formation efficiency was stable when passage 1 (P1) to P8 cells were cultured, and the stemness rate of P8 cells was close to 85.1%. EpiSCs were expanded in vitro for 25 days, the number of cells reached 2.5 × 108, and the transplantable area was approximately 75% of the total body surface area (TBSA). At 45 days, the total number of cells was approximately 30 billion, and the transplantable area was approximately the size of a volleyball court. A nude mouse wound model indicated that EpiSCs could rapidly close a wound. On postinjury day 7, the wound epithelialization rate in the cell transplantation group was significantly higher than that in the NaCl group (P < 0.05). In vitro, cell senescence increased, and telomerase activity decreased in P1 to P8 EpiSCs. In vivo, there were no solid tumors or metastatic tumors after EpiSC (P8) transplantation. In addition, the quality control of cultured cells met the clinical application criteria for cell therapy. Conclusion This preclinical study showed the stability and biosafety of human EpiSC therapy for wound repair. Graphical Abstracthttps://doi.org/10.1186/s13287-022-03202-6Epidermal stem cells (EpiSCs)Wound repairBiosafetyPreclinical |
| spellingShingle | Xiaohong Zhao Xue Li Ying Wang Yicheng Guo Yong Huang Dalun Lv Mingxing Lei Shicang Yu Gaoxing Luo Rixing Zhan Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation Stem Cell Research & Therapy Epidermal stem cells (EpiSCs) Wound repair Biosafety Preclinical |
| title | Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation |
| title_full | Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation |
| title_fullStr | Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation |
| title_full_unstemmed | Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation |
| title_short | Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation |
| title_sort | stability and biosafety of human epidermal stem cell for wound repair preclinical evaluation |
| topic | Epidermal stem cells (EpiSCs) Wound repair Biosafety Preclinical |
| url | https://doi.org/10.1186/s13287-022-03202-6 |
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