Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili production
Abstract Background Anthracnose disease caused by Colletotrichum capsici is a major disease in chili plants that is difficult to control. Actinobacteria have potential biological control agents for C. capsici because of their antifungal properties and dominant presence in soil. The study aimed to ob...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-03-01
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| Series: | Egyptian Journal of Biological Pest Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41938-023-00660-9 |
| _version_ | 1797859846256590848 |
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| author | Yulmira Yanti Hasmiandy Hamid Reflin Yaherwandi Nurbailis Ni Luh Suriani M. S. Reddy Mila Syahputri |
| author_facet | Yulmira Yanti Hasmiandy Hamid Reflin Yaherwandi Nurbailis Ni Luh Suriani M. S. Reddy Mila Syahputri |
| author_sort | Yulmira Yanti |
| collection | DOAJ |
| description | Abstract Background Anthracnose disease caused by Colletotrichum capsici is a major disease in chili plants that is difficult to control. Actinobacteria have potential biological control agents for C. capsici because of their antifungal properties and dominant presence in soil. The study aimed to obtain actinobacteria isolates that have the potential to control anthracnose in planta and increase the growth and production of chili plants. The study consisted of three stages: isolation, selection, and characterization of the best actinobacteria isolates. A total of 20 isolates of indigenous actinobacteria were isolated, and 16 isolates were successfully selected based on the results of the biosafety test. Results The in planta test showed that eight isolates could control anthracnose with 100% effectiveness. Of the eight isolates, as many as five players increased the production of chili plants by 169.51–218.53 g. Actinobacterial isolates that have the potential to control anthracnose disease in planta and increase the growth and production of chili plants are ARAI 3221, ARAC 3221, ARAC 2211, ARAC 3321, and ARTI 1312. These isolates produced indole acetic acid (IAA) with concentrations of 25.82–88.87 ppm, and four isolates were able to dissolve phosphate. Five isolates produced chitinase enzyme with the chitinolytic index of 0.32–1.78. Conclusion The introduction of actinobacteria in chili plants was also proven to extend the incubation period, reduce the incidence of disease, and reduce the severity of anthracnose disease compared to negative controls and mancozeb. Actinobacteria can suppress pathogenic microorganisms that can inhibit plant growth. Actinobacteria have the potential to increase the growth and production of chili plants. The results of 16S rRNA sequences showed that the five potential isolates were identified as Streptomyces cellulose, S. fradiae, S. olivaceus, S. pseudogriseolus, and S. griseoflavus. |
| first_indexed | 2024-04-09T21:36:13Z |
| format | Article |
| id | doaj.art-ce683e4a86e44b7f974031a6c791d782 |
| institution | Directory Open Access Journal |
| issn | 2536-9342 |
| language | English |
| last_indexed | 2024-04-09T21:36:13Z |
| publishDate | 2023-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Biological Pest Control |
| spelling | doaj.art-ce683e4a86e44b7f974031a6c791d7822023-03-26T11:17:30ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422023-03-013311910.1186/s41938-023-00660-9Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili productionYulmira Yanti0Hasmiandy Hamid1Reflin2Yaherwandi3Nurbailis4Ni Luh Suriani5M. S. Reddy6Mila Syahputri7Department of Plant Pests and Diseases, Agriculture Faculty, Universitas AndalasDepartment of Plant Pests and Diseases, Agriculture Faculty, Universitas AndalasDepartment of Plant Pests and Diseases, Agriculture Faculty, Universitas AndalasDepartment of Plant Pests and Diseases, Agriculture Faculty, Universitas AndalasDepartment of Plant Pests and Diseases, Agriculture Faculty, Universitas AndalasDepartment of Biology, Faculty of Mathematics and Natural Sciences, Udayana UniversityAsian PGPR Society for Sustainable Agriculture, Auburn UniversityDepartment of Plant Pests and Diseases, Agriculture Faculty, Universitas AndalasAbstract Background Anthracnose disease caused by Colletotrichum capsici is a major disease in chili plants that is difficult to control. Actinobacteria have potential biological control agents for C. capsici because of their antifungal properties and dominant presence in soil. The study aimed to obtain actinobacteria isolates that have the potential to control anthracnose in planta and increase the growth and production of chili plants. The study consisted of three stages: isolation, selection, and characterization of the best actinobacteria isolates. A total of 20 isolates of indigenous actinobacteria were isolated, and 16 isolates were successfully selected based on the results of the biosafety test. Results The in planta test showed that eight isolates could control anthracnose with 100% effectiveness. Of the eight isolates, as many as five players increased the production of chili plants by 169.51–218.53 g. Actinobacterial isolates that have the potential to control anthracnose disease in planta and increase the growth and production of chili plants are ARAI 3221, ARAC 3221, ARAC 2211, ARAC 3321, and ARTI 1312. These isolates produced indole acetic acid (IAA) with concentrations of 25.82–88.87 ppm, and four isolates were able to dissolve phosphate. Five isolates produced chitinase enzyme with the chitinolytic index of 0.32–1.78. Conclusion The introduction of actinobacteria in chili plants was also proven to extend the incubation period, reduce the incidence of disease, and reduce the severity of anthracnose disease compared to negative controls and mancozeb. Actinobacteria can suppress pathogenic microorganisms that can inhibit plant growth. Actinobacteria have the potential to increase the growth and production of chili plants. The results of 16S rRNA sequences showed that the five potential isolates were identified as Streptomyces cellulose, S. fradiae, S. olivaceus, S. pseudogriseolus, and S. griseoflavus.https://doi.org/10.1186/s41938-023-00660-9ActinobacteriaAnthracnoseColletotrichum capsiciChitinase enzymeIAA |
| spellingShingle | Yulmira Yanti Hasmiandy Hamid Reflin Yaherwandi Nurbailis Ni Luh Suriani M. S. Reddy Mila Syahputri Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili production Egyptian Journal of Biological Pest Control Actinobacteria Anthracnose Colletotrichum capsici Chitinase enzyme IAA |
| title | Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili production |
| title_full | Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili production |
| title_fullStr | Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili production |
| title_full_unstemmed | Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili production |
| title_short | Screening of indigenous actinobacteria as biological control agents of Colletotrichum capsici and increasing chili production |
| title_sort | screening of indigenous actinobacteria as biological control agents of colletotrichum capsici and increasing chili production |
| topic | Actinobacteria Anthracnose Colletotrichum capsici Chitinase enzyme IAA |
| url | https://doi.org/10.1186/s41938-023-00660-9 |
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