Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial Cells
The endothelial integrity is the cornerstone of the atherogenic process. Low-density lipoprotein (LDL) oxidation occurring within atheromatous plaques leads to deleterious vascular effects including endothelial cell cytotoxicity. The aim of this study was to evaluate the vascular antioxidant and cyt...
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MDPI AG
2021-12-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/1/34 |
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| author | Jonathan Bonneville Philippe Rondeau Bryan Veeren Julien Faccini Marie-Paule Gonthier Olivier Meilhac Cécile Vindis |
| author_facet | Jonathan Bonneville Philippe Rondeau Bryan Veeren Julien Faccini Marie-Paule Gonthier Olivier Meilhac Cécile Vindis |
| author_sort | Jonathan Bonneville |
| collection | DOAJ |
| description | The endothelial integrity is the cornerstone of the atherogenic process. Low-density lipoprotein (LDL) oxidation occurring within atheromatous plaques leads to deleterious vascular effects including endothelial cell cytotoxicity. The aim of this study was to evaluate the vascular antioxidant and cytoprotective effects of polyphenol-rich extracts from two medicinal plants from the Reunion Island: <i>Antirhea borbonica (A. borbonica)</i>, <i>Doratoxylon apetalum (D. apetalum)</i>. The polyphenol-rich extracts were obtained after dissolving each dry plant powder in an aqueous acetonic solution. Quantification of polyphenol content was achieved by the Folin–Ciocalteu assay and total phenol content was expressed as g gallic acid equivalent/100 g plant powder (GAE). Human vascular endothelial cells were incubated with increasing concentrations of polyphenols (1–50 µM GAE) before stimulation with oxidized low-density lipoproteins (oxLDLs). LDL oxidation was assessed by quantification of hydroperoxides and thiobarbituric acid reactive substances (TBARS). Intracellular oxidative stress and antioxidant activity (catalase and superoxide dismutase) were measured after stimulation with oxLDLs. Cell viability and apoptosis were quantified using different assays (MTT, Annexin V staining, cytochrome C release, caspase 3 activation and TUNEL test). <i>A. borbonica</i> and <i>D. apetalum</i> displayed high levels of polyphenols and limited LDL oxidation as well as oxLDL-induced intracellular oxidative stress in endothelial cells. Polyphenol extracts of <i>A. borbonica</i> and <i>D. apetalum</i> exerted a protective effect against oxLDL-induced cell apoptosis in a dose-dependent manner (10, 25, and 50 µM GAE) similar to that observed for curcumin, used as positive control. All together, these results showed significant antioxidant and antiapoptotic properties for two plants of the Reunion Island pharmacopeia, <i>A. borbonica</i> and <i>D. apetalum,</i> suggesting their therapeutic potential to prevent cardiovascular diseases by limiting LDL oxidation and protecting the endothelium. |
| first_indexed | 2024-03-10T03:02:10Z |
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| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:02:10Z |
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| spelling | doaj.art-ce6c9b67783a412eaef4946873748ac52023-11-23T12:46:17ZengMDPI AGAntioxidants2076-39212021-12-011113410.3390/antiox11010034Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial CellsJonathan Bonneville0Philippe Rondeau1Bryan Veeren2Julien Faccini3Marie-Paule Gonthier4Olivier Meilhac5Cécile Vindis6Clinical Investigation Center (CIC) 1436, INSERM 1048, 31400 Toulouse, FranceINSERM, UMR 1188 Diabete athérothrombose Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 La Réunion, FranceINSERM, UMR 1188 Diabete athérothrombose Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 La Réunion, FranceClinical Investigation Center (CIC) 1436, INSERM 1048, 31400 Toulouse, FranceINSERM, UMR 1188 Diabete athérothrombose Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 La Réunion, FranceINSERM, UMR 1188 Diabete athérothrombose Réunion Océan Indien (DéTROI), Université de La Réunion, 97400 La Réunion, FranceClinical Investigation Center (CIC) 1436, INSERM 1048, 31400 Toulouse, FranceThe endothelial integrity is the cornerstone of the atherogenic process. Low-density lipoprotein (LDL) oxidation occurring within atheromatous plaques leads to deleterious vascular effects including endothelial cell cytotoxicity. The aim of this study was to evaluate the vascular antioxidant and cytoprotective effects of polyphenol-rich extracts from two medicinal plants from the Reunion Island: <i>Antirhea borbonica (A. borbonica)</i>, <i>Doratoxylon apetalum (D. apetalum)</i>. The polyphenol-rich extracts were obtained after dissolving each dry plant powder in an aqueous acetonic solution. Quantification of polyphenol content was achieved by the Folin–Ciocalteu assay and total phenol content was expressed as g gallic acid equivalent/100 g plant powder (GAE). Human vascular endothelial cells were incubated with increasing concentrations of polyphenols (1–50 µM GAE) before stimulation with oxidized low-density lipoproteins (oxLDLs). LDL oxidation was assessed by quantification of hydroperoxides and thiobarbituric acid reactive substances (TBARS). Intracellular oxidative stress and antioxidant activity (catalase and superoxide dismutase) were measured after stimulation with oxLDLs. Cell viability and apoptosis were quantified using different assays (MTT, Annexin V staining, cytochrome C release, caspase 3 activation and TUNEL test). <i>A. borbonica</i> and <i>D. apetalum</i> displayed high levels of polyphenols and limited LDL oxidation as well as oxLDL-induced intracellular oxidative stress in endothelial cells. Polyphenol extracts of <i>A. borbonica</i> and <i>D. apetalum</i> exerted a protective effect against oxLDL-induced cell apoptosis in a dose-dependent manner (10, 25, and 50 µM GAE) similar to that observed for curcumin, used as positive control. All together, these results showed significant antioxidant and antiapoptotic properties for two plants of the Reunion Island pharmacopeia, <i>A. borbonica</i> and <i>D. apetalum,</i> suggesting their therapeutic potential to prevent cardiovascular diseases by limiting LDL oxidation and protecting the endothelium.https://www.mdpi.com/2076-3921/11/1/34<i>Antirhea borbonica</i><i>Doratoxylon apetalum</i>polyphenoloxidized LDLvascular cell |
| spellingShingle | Jonathan Bonneville Philippe Rondeau Bryan Veeren Julien Faccini Marie-Paule Gonthier Olivier Meilhac Cécile Vindis Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial Cells Antioxidants <i>Antirhea borbonica</i> <i>Doratoxylon apetalum</i> polyphenol oxidized LDL vascular cell |
| title | Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial Cells |
| title_full | Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial Cells |
| title_fullStr | Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial Cells |
| title_full_unstemmed | Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial Cells |
| title_short | Antioxidant and Cytoprotective Properties of Polyphenol-Rich Extracts from <em>Antirhea borbonica</em> and <em>Doratoxylon apetalum</em> against Atherogenic Lipids in Human Endothelial Cells |
| title_sort | antioxidant and cytoprotective properties of polyphenol rich extracts from em antirhea borbonica em and em doratoxylon apetalum em against atherogenic lipids in human endothelial cells |
| topic | <i>Antirhea borbonica</i> <i>Doratoxylon apetalum</i> polyphenol oxidized LDL vascular cell |
| url | https://www.mdpi.com/2076-3921/11/1/34 |
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