The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus
Developing T cells commit to either CD4/helper or CD8/cytotoxic lineage in the thymus, but how CD4 and CD8 coreceptors and TCR signaling dictate this selection process is still unclear. Here the authors use single cell RNA sequencing of mouse thymocytes to show that, in selection intermediates, TCR...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-20306-w |
| _version_ | 1818842935816355840 |
|---|---|
| author | Mohammad M. Karimi Ya Guo Xiaokai Cui Husayn A. Pallikonda Veronika Horková Yi-Fang Wang Sara Ruiz Gil Gustavo Rodriguez-Esteban Irene Robles-Rebollo Ludovica Bruno Radina Georgieva Bhavik Patel James Elliott Marian H. Dore Danielle Dauphars Michael S. Krangel Boris Lenhard Holger Heyn Amanda G. Fisher Ondřej Štěpánek Matthias Merkenschlager |
| author_facet | Mohammad M. Karimi Ya Guo Xiaokai Cui Husayn A. Pallikonda Veronika Horková Yi-Fang Wang Sara Ruiz Gil Gustavo Rodriguez-Esteban Irene Robles-Rebollo Ludovica Bruno Radina Georgieva Bhavik Patel James Elliott Marian H. Dore Danielle Dauphars Michael S. Krangel Boris Lenhard Holger Heyn Amanda G. Fisher Ondřej Štěpánek Matthias Merkenschlager |
| author_sort | Mohammad M. Karimi |
| collection | DOAJ |
| description | Developing T cells commit to either CD4/helper or CD8/cytotoxic lineage in the thymus, but how CD4 and CD8 coreceptors and TCR signaling dictate this selection process is still unclear. Here the authors use single cell RNA sequencing of mouse thymocytes to show that, in selection intermediates, TCR signaling strength informs coreceptor expression timing. |
| first_indexed | 2024-12-19T04:49:53Z |
| format | Article |
| id | doaj.art-ce6d54e4a05749d99545339b720e286a |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T04:49:53Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ce6d54e4a05749d99545339b720e286a2022-12-21T20:35:23ZengNature PortfolioNature Communications2041-17232021-01-0112111410.1038/s41467-020-20306-wThe order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymusMohammad M. Karimi0Ya Guo1Xiaokai Cui2Husayn A. Pallikonda3Veronika Horková4Yi-Fang Wang5Sara Ruiz Gil6Gustavo Rodriguez-Esteban7Irene Robles-Rebollo8Ludovica Bruno9Radina Georgieva10Bhavik Patel11James Elliott12Marian H. Dore13Danielle Dauphars14Michael S. Krangel15Boris Lenhard16Holger Heyn17Amanda G. Fisher18Ondřej Štěpánek19Matthias Merkenschlager20MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonLaboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of SciencesMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonCNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)CNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonDepartment of Immunology, Duke University Medical CenterDepartment of Immunology, Duke University Medical CenterMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonCNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology (BIST)MRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonLaboratory of Adaptive Immunity, Institute of Molecular Genetics of the Czech Academy of SciencesMRC London Institute of Medical Sciences, Institute of Clinical Sciences, Faculty of Medicine, Imperial College LondonDeveloping T cells commit to either CD4/helper or CD8/cytotoxic lineage in the thymus, but how CD4 and CD8 coreceptors and TCR signaling dictate this selection process is still unclear. Here the authors use single cell RNA sequencing of mouse thymocytes to show that, in selection intermediates, TCR signaling strength informs coreceptor expression timing.https://doi.org/10.1038/s41467-020-20306-w |
| spellingShingle | Mohammad M. Karimi Ya Guo Xiaokai Cui Husayn A. Pallikonda Veronika Horková Yi-Fang Wang Sara Ruiz Gil Gustavo Rodriguez-Esteban Irene Robles-Rebollo Ludovica Bruno Radina Georgieva Bhavik Patel James Elliott Marian H. Dore Danielle Dauphars Michael S. Krangel Boris Lenhard Holger Heyn Amanda G. Fisher Ondřej Štěpánek Matthias Merkenschlager The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus Nature Communications |
| title | The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus |
| title_full | The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus |
| title_fullStr | The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus |
| title_full_unstemmed | The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus |
| title_short | The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus |
| title_sort | order and logic of cd4 versus cd8 lineage choice and differentiation in mouse thymus |
| url | https://doi.org/10.1038/s41467-020-20306-w |
| work_keys_str_mv | AT mohammadmkarimi theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT yaguo theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT xiaokaicui theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT husaynapallikonda theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT veronikahorkova theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT yifangwang theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT sararuizgil theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT gustavorodriguezesteban theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT ireneroblesrebollo theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT ludovicabruno theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT radinageorgieva theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT bhavikpatel theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT jameselliott theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT marianhdore theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT danielledauphars theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT michaelskrangel theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT borislenhard theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT holgerheyn theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT amandagfisher theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT ondrejstepanek theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT matthiasmerkenschlager theorderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT mohammadmkarimi orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT yaguo orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT xiaokaicui orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT husaynapallikonda orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT veronikahorkova orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT yifangwang orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT sararuizgil orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT gustavorodriguezesteban orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT ireneroblesrebollo orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT ludovicabruno orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT radinageorgieva orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT bhavikpatel orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT jameselliott orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT marianhdore orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT danielledauphars orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT michaelskrangel orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT borislenhard orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT holgerheyn orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT amandagfisher orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT ondrejstepanek orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus AT matthiasmerkenschlager orderandlogicofcd4versuscd8lineagechoiceanddifferentiationinmousethymus |