Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats
Introduction Prolonged shortages of protein in the diet significantly alter the composition and content of polyunsaturated fatty acids (PUFA) in tissues and body fluids. One of nutritional factors which may reduce negative effects of protein malnutrition might be vitamin B<sub>2</sub> du...
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| Language: | English |
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Institute of Rural Health
2017-05-01
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| Series: | Annals of Agricultural and Environmental Medicine |
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| Online Access: | http://www.journalssystem.com/aaem/Influence-of-protein-deficient-diet-vitamin-B-sub-2-sub-supplementation-and-physical-training-on-serum-composition-of-polyunsaturated-fatty-acids-PUFAs-in-rats,72565,0,2.html |
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| author | Aneta Lewicka Sławomir Lewicki Anna Kłos Bogdan Dębski Tomasz Kurył Jerzy Bertrandt |
| author_facet | Aneta Lewicka Sławomir Lewicki Anna Kłos Bogdan Dębski Tomasz Kurył Jerzy Bertrandt |
| author_sort | Aneta Lewicka |
| collection | DOAJ |
| description | Introduction
Prolonged shortages of protein in the diet significantly alter the composition and content of polyunsaturated fatty acids (PUFA) in tissues and body fluids. One of nutritional factors which may reduce negative effects of protein malnutrition might be vitamin B<sub>2</sub> due to its influence on lipids metabolism.
Objective
The aim of the study was to investigate the influence of low protein (LP) diet enriched with vitamin B<sub>2</sub> on the content and composition of PUFA in the blood serum of rats treated with dosed physical exercise.
Material and Methods
The experiment was carried out for 3 months on 72 growing male Wistar rats divided into 5 groups. Animals were fed ad libitum on a diet with an energy value of 350 kcal/100 g, in which 4.5% of the energy was provided by protein. In the control diet, 20% of the energy was provided by protein. Two groups were fed the diet enriched with vitamin B2. The two groups of tested animals were trained for 5 days a week.
Results
LP diet caused a decrease in α-linolenic acid (ALA) after 30 days, and a decrease in docosahexaenoic acid (DHA) after 60 days of experiment, compared with rats fed the control diet. After 60 and 90 days of the experiment, a significant decrease was noted in arachidonic acid (AA) in serum of trained rats, compared with sedentary rats fed the LP diet. Physical activity increased LA (mainly on day 30), EPA (on day 90) and reduced AA content (on day 90) in serum of rats fed the LP diet. B2 supplementation in the trained LP group did not change the EPA and AA dependence; however, there was a decrease in LA content in comparison to the non-supplemented trained group.
Conclusions
Results of this study suggest that all investigated factors (protein deficiency, physical exercise and supplementation of vitamin B2) have significant impact on PUFA composition of serum in rats. |
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| format | Article |
| id | doaj.art-ce6ef0b370624e1d9de5fea3866fdae4 |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-04-12T10:18:34Z |
| publishDate | 2017-05-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-ce6ef0b370624e1d9de5fea3866fdae42022-12-22T03:37:09ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632017-05-0124218518910.5604/12321966.122839172565Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in ratsAneta Lewicka0Sławomir Lewicki1Anna Kłos2Bogdan Dębski3Tomasz Kurył4Jerzy Bertrandt5Department of Hygiene and Physiology, Military Institute of Hygiene and Epidemiology, Warsaw, PolandDepartment of Regenerative Medicine, Military Institute of Hygiene and Epidemiology, Warsaw, PolandDepartment of Hygiene and Physiology, Military Institute of Hygiene and Epidemiology, Warsaw, PolandDepartment of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, PolandDepartment of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, PolandDepartment of Hygiene and Physiology, Military Institute of Hygiene and Epidemiology, Warsaw, PolandIntroduction Prolonged shortages of protein in the diet significantly alter the composition and content of polyunsaturated fatty acids (PUFA) in tissues and body fluids. One of nutritional factors which may reduce negative effects of protein malnutrition might be vitamin B<sub>2</sub> due to its influence on lipids metabolism. Objective The aim of the study was to investigate the influence of low protein (LP) diet enriched with vitamin B<sub>2</sub> on the content and composition of PUFA in the blood serum of rats treated with dosed physical exercise. Material and Methods The experiment was carried out for 3 months on 72 growing male Wistar rats divided into 5 groups. Animals were fed ad libitum on a diet with an energy value of 350 kcal/100 g, in which 4.5% of the energy was provided by protein. In the control diet, 20% of the energy was provided by protein. Two groups were fed the diet enriched with vitamin B2. The two groups of tested animals were trained for 5 days a week. Results LP diet caused a decrease in α-linolenic acid (ALA) after 30 days, and a decrease in docosahexaenoic acid (DHA) after 60 days of experiment, compared with rats fed the control diet. After 60 and 90 days of the experiment, a significant decrease was noted in arachidonic acid (AA) in serum of trained rats, compared with sedentary rats fed the LP diet. Physical activity increased LA (mainly on day 30), EPA (on day 90) and reduced AA content (on day 90) in serum of rats fed the LP diet. B2 supplementation in the trained LP group did not change the EPA and AA dependence; however, there was a decrease in LA content in comparison to the non-supplemented trained group. Conclusions Results of this study suggest that all investigated factors (protein deficiency, physical exercise and supplementation of vitamin B2) have significant impact on PUFA composition of serum in rats.http://www.journalssystem.com/aaem/Influence-of-protein-deficient-diet-vitamin-B-sub-2-sub-supplementation-and-physical-training-on-serum-composition-of-polyunsaturated-fatty-acids-PUFAs-in-rats,72565,0,2.htmlProtein malnutritionvitamin B2trainingPUFA |
| spellingShingle | Aneta Lewicka Sławomir Lewicki Anna Kłos Bogdan Dębski Tomasz Kurył Jerzy Bertrandt Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats Annals of Agricultural and Environmental Medicine Protein malnutrition vitamin B2 training PUFA |
| title | Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats |
| title_full | Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats |
| title_fullStr | Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats |
| title_full_unstemmed | Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats |
| title_short | Influence of protein deficient diet, vitamin B<sub>2</sub> supplementation and physical training on serum composition of polyunsaturated fatty acids (PUFAs) in rats |
| title_sort | influence of protein deficient diet vitamin b sub 2 sub supplementation and physical training on serum composition of polyunsaturated fatty acids pufas in rats |
| topic | Protein malnutrition vitamin B2 training PUFA |
| url | http://www.journalssystem.com/aaem/Influence-of-protein-deficient-diet-vitamin-B-sub-2-sub-supplementation-and-physical-training-on-serum-composition-of-polyunsaturated-fatty-acids-PUFAs-in-rats,72565,0,2.html |
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