The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 Cells
Prebiotics and probiotics have gained much attention in the pursuit of a healthy life. Recently, postbiotics have been spotlighted as next-generation compounds that can improve health. Postbiotics are designated into non-viable, inactivated, and ghost probiotics, and linked to several health benefit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/24/12519 |
| _version_ | 1797461725155426304 |
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| author | Yeeun Kim Hak Jun Kim Keunho Ji |
| author_facet | Yeeun Kim Hak Jun Kim Keunho Ji |
| author_sort | Yeeun Kim |
| collection | DOAJ |
| description | Prebiotics and probiotics have gained much attention in the pursuit of a healthy life. Recently, postbiotics have been spotlighted as next-generation compounds that can improve health. Postbiotics are designated into non-viable, inactivated, and ghost probiotics, and linked to several health benefits for the gut, immune system, and various other aspects of health. This study investigated the anti-proliferation effects of postbiotics against HT-29 cells, a colon cancer cell line. The postbiotics were produced by the ultrasonication method from two <i>Lactobacillus</i> strains (<i>Lactobacillus</i> sp. La1, and La2) and designated to Pobt-La1 and Pobt-La2, respectively, and non-viability was confirmed on the plate media. The anti-proliferation effect was concentration-dependent. The HT-29 cells showed viabilities of 39% and 49% when treated with 300 µL/mL of Pobt-La1 and Pobt-La2, respectively. During observation of the morphological changes of HT-29 cells when treated with IC50, a cell nucleus was not observed but cell condensation was observed. Moreover, in comparison with the control group, a reduced number of cells were observed. Based on these results, it considered that the postbiotic compounds from <i>Lactobacillus</i> La1 and La2 could provide crucial information in the development of anticancer research. Through further research, it would be beneficial to investigate the possibility of using these postbiotics (Pobt-La1 and -La2) as an anticancer drug. |
| first_indexed | 2024-03-09T17:23:24Z |
| format | Article |
| id | doaj.art-ce71e6888ed14597a5d6b196c695cdaa |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T17:23:24Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-ce71e6888ed14597a5d6b196c695cdaa2023-11-24T12:59:59ZengMDPI AGApplied Sciences2076-34172022-12-0112241251910.3390/app122412519The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 CellsYeeun Kim0Hak Jun Kim1Keunho Ji2Department of Microbiology, Pukyong National University, Busan 48513, Republic of KoreaDepartment of Chemistry, Pukyong National University, Busan 48513, Republic of KoreaBasic Science Research Institute, Pukyong National University, Busan 48513, Republic of KoreaPrebiotics and probiotics have gained much attention in the pursuit of a healthy life. Recently, postbiotics have been spotlighted as next-generation compounds that can improve health. Postbiotics are designated into non-viable, inactivated, and ghost probiotics, and linked to several health benefits for the gut, immune system, and various other aspects of health. This study investigated the anti-proliferation effects of postbiotics against HT-29 cells, a colon cancer cell line. The postbiotics were produced by the ultrasonication method from two <i>Lactobacillus</i> strains (<i>Lactobacillus</i> sp. La1, and La2) and designated to Pobt-La1 and Pobt-La2, respectively, and non-viability was confirmed on the plate media. The anti-proliferation effect was concentration-dependent. The HT-29 cells showed viabilities of 39% and 49% when treated with 300 µL/mL of Pobt-La1 and Pobt-La2, respectively. During observation of the morphological changes of HT-29 cells when treated with IC50, a cell nucleus was not observed but cell condensation was observed. Moreover, in comparison with the control group, a reduced number of cells were observed. Based on these results, it considered that the postbiotic compounds from <i>Lactobacillus</i> La1 and La2 could provide crucial information in the development of anticancer research. Through further research, it would be beneficial to investigate the possibility of using these postbiotics (Pobt-La1 and -La2) as an anticancer drug.https://www.mdpi.com/2076-3417/12/24/12519probioticspostbioticsHT-29 cellsanticancer<i>Lactobacillus</i> |
| spellingShingle | Yeeun Kim Hak Jun Kim Keunho Ji The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 Cells Applied Sciences probiotics postbiotics HT-29 cells anticancer <i>Lactobacillus</i> |
| title | The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 Cells |
| title_full | The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 Cells |
| title_fullStr | The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 Cells |
| title_full_unstemmed | The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 Cells |
| title_short | The Proliferation Inhibitory Effect of Postbiotics Prepared from Probiotics with Antioxidant Activity against HT-29 Cells |
| title_sort | proliferation inhibitory effect of postbiotics prepared from probiotics with antioxidant activity against ht 29 cells |
| topic | probiotics postbiotics HT-29 cells anticancer <i>Lactobacillus</i> |
| url | https://www.mdpi.com/2076-3417/12/24/12519 |
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