| Summary: | Essential oil from the leaves of <i>Artemisia vulgaris</i> L. (Compositae) cultivated in Brazil was investigated for its chemical composition and biological activities including antibacterial, antifungal, and anthelmintic. The constituents of essential oils isolated by hydro-distillation were examined by GC-MS and a total of 18 components were identified. The essential oil was dominated by oxygenated sesquiterpenes (44.4%), sesquiterpene hydrocarbons (33.3%), and oxygenated monoterpenes (16.6%). Caryophyllene (37.45%), germacrene D (16.17%), and humulene (13.66%) were the major components. The essential oils from <i>A. vulgaris</i> showed bactericidal and fungicidal properties against <i>Staphylococcus aureus</i> and <i>Candida albicans</i>, respectively. Anthelmintic activity against <i>Haemonchus contortus</i> was absent in this essential oil. Altogether above results indicate that essential oils from <i>A. vulgaris</i> can be used for various medicinal purposes.
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