Obesity modulates cell-cell interactions during ovarian folliculogenesis
Summary: Obesity is known to affect female reproduction, as evidenced by obese patients suffering from subfertility and abnormal oogenesis. However, the underlying mechanisms by which obesity impairs folliculogenesis are poorly documented. Here, we performed comprehensive single-cell transcriptome a...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221015972 |
| _version_ | 1830168934049906688 |
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| author | Xin Long Qiuyun Yang Jingjing Qian Huiying Yao Rui Yan Xin Cheng Qiancheng Zhang Chan Gu Fei Gao Hongmei Wang Lin Zhang Fan Guo |
| author_facet | Xin Long Qiuyun Yang Jingjing Qian Huiying Yao Rui Yan Xin Cheng Qiancheng Zhang Chan Gu Fei Gao Hongmei Wang Lin Zhang Fan Guo |
| author_sort | Xin Long |
| collection | DOAJ |
| description | Summary: Obesity is known to affect female reproduction, as evidenced by obese patients suffering from subfertility and abnormal oogenesis. However, the underlying mechanisms by which obesity impairs folliculogenesis are poorly documented. Here, we performed comprehensive single-cell transcriptome analysis in both regular diet (RD) and obese mouse models to systematically uncover how obesity affects ovarian follicle cells and their interactions. We found an increased proportion of Inhbb highly expressed granulosa cells (GCs) among all the GC subpopulations in obese mice. Under obese conditions, excessive androgen secreted from endocrine theca cells (ETCs) may contribute to the imbalanced change of GC subtypes through ETCs-GCs interactions. This is alleviated by enzalutamide, an androgen receptor antagonist. We also identified and confirmed typical GC markers, such as Marcks and Prkar2b, for sensitive evaluation of female fertility in obesity. These data represent a resource for studying transcriptional networks and cell-cell interactions during folliculogenesis under physiological and pathological conditions. |
| first_indexed | 2024-12-17T17:12:14Z |
| format | Article |
| id | doaj.art-ce74a901b51847b4a440c19829caa38d |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-17T17:12:14Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-ce74a901b51847b4a440c19829caa38d2022-12-21T21:40:03ZengElsevieriScience2589-00422022-01-01251103627Obesity modulates cell-cell interactions during ovarian folliculogenesisXin Long0Qiuyun Yang1Jingjing Qian2Huiying Yao3Rui Yan4Xin Cheng5Qiancheng Zhang6Chan Gu7Fei Gao8Hongmei Wang9Lin Zhang10Fan Guo11State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, ChinaWest China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaWest China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, China; West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, China; Corresponding authorState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing 100101, China; Beijing Institute for Stem Cell and Regenerative Medicine, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Corresponding authorSummary: Obesity is known to affect female reproduction, as evidenced by obese patients suffering from subfertility and abnormal oogenesis. However, the underlying mechanisms by which obesity impairs folliculogenesis are poorly documented. Here, we performed comprehensive single-cell transcriptome analysis in both regular diet (RD) and obese mouse models to systematically uncover how obesity affects ovarian follicle cells and their interactions. We found an increased proportion of Inhbb highly expressed granulosa cells (GCs) among all the GC subpopulations in obese mice. Under obese conditions, excessive androgen secreted from endocrine theca cells (ETCs) may contribute to the imbalanced change of GC subtypes through ETCs-GCs interactions. This is alleviated by enzalutamide, an androgen receptor antagonist. We also identified and confirmed typical GC markers, such as Marcks and Prkar2b, for sensitive evaluation of female fertility in obesity. These data represent a resource for studying transcriptional networks and cell-cell interactions during folliculogenesis under physiological and pathological conditions.http://www.sciencedirect.com/science/article/pii/S2589004221015972Biological sciencesEndocrinologyCell biologyTranscriptomics |
| spellingShingle | Xin Long Qiuyun Yang Jingjing Qian Huiying Yao Rui Yan Xin Cheng Qiancheng Zhang Chan Gu Fei Gao Hongmei Wang Lin Zhang Fan Guo Obesity modulates cell-cell interactions during ovarian folliculogenesis iScience Biological sciences Endocrinology Cell biology Transcriptomics |
| title | Obesity modulates cell-cell interactions during ovarian folliculogenesis |
| title_full | Obesity modulates cell-cell interactions during ovarian folliculogenesis |
| title_fullStr | Obesity modulates cell-cell interactions during ovarian folliculogenesis |
| title_full_unstemmed | Obesity modulates cell-cell interactions during ovarian folliculogenesis |
| title_short | Obesity modulates cell-cell interactions during ovarian folliculogenesis |
| title_sort | obesity modulates cell cell interactions during ovarian folliculogenesis |
| topic | Biological sciences Endocrinology Cell biology Transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221015972 |
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