An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins
Gout is a condition that affects over 8 million Americans. This condition is characterized by severe pain, and in more advanced cases, bone erosion and joint destruction. This study explores the fabrication and characterization of an optical, enzymatic urate biosensor for gout management, and the op...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-02-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/4/959 |
| _version_ | 1811187986919849984 |
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| author | Tokunbo Falohun Michael J. McShane |
| author_facet | Tokunbo Falohun Michael J. McShane |
| author_sort | Tokunbo Falohun |
| collection | DOAJ |
| description | Gout is a condition that affects over 8 million Americans. This condition is characterized by severe pain, and in more advanced cases, bone erosion and joint destruction. This study explores the fabrication and characterization of an optical, enzymatic urate biosensor for gout management, and the optimization of the biosensor response through the tuning of hydrogel matrix properties. Sensors were fabricated through the co-immobilization of oxygen-quenched phosphorescent probes with an oxidoreductase within a biocompatible copolymer hydrogel matrix. Characterization of the spectral properties and hydrogel swelling was conducted, as well as evaluation of the response sensitivity and long-term stability of the urate biosensor. The findings indicate that increased acrylamide concentration improved the biosensor response by yielding an increased sensitivity and reduced lower limit of detection. However, the repeatability and stability tests highlighted some possible areas of improvement, with a consistent response drift observed during repeatability testing and a reduction in response seen after long-term storage tests. Overall, this study demonstrates the potential of an on-demand, patient-friendly gout management tool, while paving the way for a future multi-analyte biosensor based on this sensing platform. |
| first_indexed | 2024-04-11T14:11:51Z |
| format | Article |
| id | doaj.art-ce74d933858d48848c8822139c1ae6d4 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T14:11:51Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-ce74d933858d48848c8822139c1ae6d42022-12-22T04:19:41ZengMDPI AGSensors1424-82202020-02-0120495910.3390/s20040959s20040959An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime MetalloporphyrinsTokunbo Falohun0Michael J. McShane1Department of Biomedical Engineering, 5045 Emerging Technologies Building, 3120 TAMU, Texas A&M University, College Station, TX 77843, USADepartment of Biomedical Engineering, 5045 Emerging Technologies Building, 3120 TAMU, Texas A&M University, College Station, TX 77843, USAGout is a condition that affects over 8 million Americans. This condition is characterized by severe pain, and in more advanced cases, bone erosion and joint destruction. This study explores the fabrication and characterization of an optical, enzymatic urate biosensor for gout management, and the optimization of the biosensor response through the tuning of hydrogel matrix properties. Sensors were fabricated through the co-immobilization of oxygen-quenched phosphorescent probes with an oxidoreductase within a biocompatible copolymer hydrogel matrix. Characterization of the spectral properties and hydrogel swelling was conducted, as well as evaluation of the response sensitivity and long-term stability of the urate biosensor. The findings indicate that increased acrylamide concentration improved the biosensor response by yielding an increased sensitivity and reduced lower limit of detection. However, the repeatability and stability tests highlighted some possible areas of improvement, with a consistent response drift observed during repeatability testing and a reduction in response seen after long-term storage tests. Overall, this study demonstrates the potential of an on-demand, patient-friendly gout management tool, while paving the way for a future multi-analyte biosensor based on this sensing platform.https://www.mdpi.com/1424-8220/20/4/959urategoutoptical biosensorsmetalloporphyrinsphosphorescence |
| spellingShingle | Tokunbo Falohun Michael J. McShane An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins Sensors urate gout optical biosensors metalloporphyrins phosphorescence |
| title | An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins |
| title_full | An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins |
| title_fullStr | An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins |
| title_full_unstemmed | An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins |
| title_short | An Optical Urate Biosensor Based on Urate Oxidase and Long-Lifetime Metalloporphyrins |
| title_sort | optical urate biosensor based on urate oxidase and long lifetime metalloporphyrins |
| topic | urate gout optical biosensors metalloporphyrins phosphorescence |
| url | https://www.mdpi.com/1424-8220/20/4/959 |
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