POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) Liver
Highlights: • Red okra pods have a potential antioxidant to protect against DMBA-induced damage in a rat’s liver. • The liver protection with ROE decreased reversible and irreversible cellular damage from 51.8% to 35%, 27.3%, and 18.9%. Abstract Background: Okra (Abelmonchus esculentus (L...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Medicine, Universitas Airlangga
2024-01-01
|
| Series: | Majalah Biomorfologi |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/MBIO/article/view/53448 |
| _version_ | 1827352858468548608 |
|---|---|
| author | Lukiteswari Dyah Tri Hapsari Kusuma Eko Purwantari Indri Safitri Mukono Sri Puji Astuti Wahyuningsih Nadyatul Ilma Indah Savira |
| author_facet | Lukiteswari Dyah Tri Hapsari Kusuma Eko Purwantari Indri Safitri Mukono Sri Puji Astuti Wahyuningsih Nadyatul Ilma Indah Savira |
| author_sort | Lukiteswari Dyah Tri Hapsari |
| collection | DOAJ |
| description | Highlights:
• Red okra pods have a potential antioxidant to protect against DMBA-induced damage in a rat’s liver.
• The liver protection with ROE decreased reversible and irreversible cellular damage from 51.8% to 35%, 27.3%, and 18.9%.
Abstract
Background: Okra (Abelmonchus esculentus (L.) Moench) is a plant that has potential for humans and health because it contains high antioxidants such as polyphenols. Objective: This study aimed to analyze the red okra pods’ ethanol extract (ROE) antioxidant potential to protect rat (Rattus norvegicus L.) liver against damage by induction of 7, 12-dimethyl-benz[a]anthracene (DMBA). Material and Method: The material used was various doses of red okra pods with ethanol as solvent. Twenty-five female rats (4 weeks, body weight 140—150 g) were divided into five groups: negative control (receiving a single dose of 0.5 mL corn oil as DMBA solvent), positive control (receiving a single dose induction of DMBA 80 mg/kg BW dissolved in 0.5 mL corn oil), and treatment groups 1, 2, 3 (receiving a single dose of DMBA 80 mg/kg BW dissolved in 0.5 mL corn oil and ROE of 50, 100, and 200 mg/kg BW, respectively, daily for 35 days). On day 36, the livers were removed and prepared for histopathological observation using hematoxylin-eosin staining. The method of histopathological score was determined using an ordinal score. Result: The data were analyzed statistically (p>0.05). All ROE doses showed a significant decrease in the histopathological score of rats’ liver damage caused by DMBA-induced. The liver protection with ROE reduced the percentages of reversible and irreversible cellular damage from 51.8% to 35%, 27.3%, and 18.9%, respectively. Conclusion: The red okra pod ethanol extract can protect against DMBA-induced liver damage in rats. |
| first_indexed | 2024-03-08T03:13:09Z |
| format | Article |
| id | doaj.art-ce74dfd6e162475ba659a953fb509ce4 |
| institution | Directory Open Access Journal |
| issn | 0215-8833 2716-0920 |
| language | English |
| last_indexed | 2024-03-08T03:13:09Z |
| publishDate | 2024-01-01 |
| publisher | Faculty of Medicine, Universitas Airlangga |
| record_format | Article |
| series | Majalah Biomorfologi |
| spelling | doaj.art-ce74dfd6e162475ba659a953fb509ce42024-02-13T02:29:30ZengFaculty of Medicine, Universitas AirlanggaMajalah Biomorfologi0215-88332716-09202024-01-01341101810.20473/mbiom.v34i1.2024.10-1851610POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) LiverLukiteswari Dyah Tri Hapsari0Kusuma Eko Purwantari1https://orcid.org/0000-0001-7416-6714Indri Safitri Mukono2https://orcid.org/0000-0003-3565-0719Sri Puji Astuti Wahyuningsih3https://orcid.org/0000-0003-1027-7110Nadyatul Ilma Indah Savira4Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Anatomy, Histology and Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Physiology and Biochemistry, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.Department of Biology, Faculty of Sains and Technology, Universitas Airlangga, Surabaya, Indonesia.Biology Education, Faculty of Teacher Training and Education, University of Jember, Jember, Indonesia.Highlights: • Red okra pods have a potential antioxidant to protect against DMBA-induced damage in a rat’s liver. • The liver protection with ROE decreased reversible and irreversible cellular damage from 51.8% to 35%, 27.3%, and 18.9%. Abstract Background: Okra (Abelmonchus esculentus (L.) Moench) is a plant that has potential for humans and health because it contains high antioxidants such as polyphenols. Objective: This study aimed to analyze the red okra pods’ ethanol extract (ROE) antioxidant potential to protect rat (Rattus norvegicus L.) liver against damage by induction of 7, 12-dimethyl-benz[a]anthracene (DMBA). Material and Method: The material used was various doses of red okra pods with ethanol as solvent. Twenty-five female rats (4 weeks, body weight 140—150 g) were divided into five groups: negative control (receiving a single dose of 0.5 mL corn oil as DMBA solvent), positive control (receiving a single dose induction of DMBA 80 mg/kg BW dissolved in 0.5 mL corn oil), and treatment groups 1, 2, 3 (receiving a single dose of DMBA 80 mg/kg BW dissolved in 0.5 mL corn oil and ROE of 50, 100, and 200 mg/kg BW, respectively, daily for 35 days). On day 36, the livers were removed and prepared for histopathological observation using hematoxylin-eosin staining. The method of histopathological score was determined using an ordinal score. Result: The data were analyzed statistically (p>0.05). All ROE doses showed a significant decrease in the histopathological score of rats’ liver damage caused by DMBA-induced. The liver protection with ROE reduced the percentages of reversible and irreversible cellular damage from 51.8% to 35%, 27.3%, and 18.9%, respectively. Conclusion: The red okra pod ethanol extract can protect against DMBA-induced liver damage in rats.https://e-journal.unair.ac.id/MBIO/article/view/53448antioxidantdmbahealthy and affordablehistopathology liverred okra ethanol extract |
| spellingShingle | Lukiteswari Dyah Tri Hapsari Kusuma Eko Purwantari Indri Safitri Mukono Sri Puji Astuti Wahyuningsih Nadyatul Ilma Indah Savira POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) Liver Majalah Biomorfologi antioxidant dmba healthy and affordable histopathology liver red okra ethanol extract |
| title | POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) Liver |
| title_full | POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) Liver |
| title_fullStr | POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) Liver |
| title_full_unstemmed | POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) Liver |
| title_short | POTENTIAL OF RED OKRA (Abelmoschus esculentus (L.) Moench) ETHANOL EXTRACT TO PROTECT AGAINST 7, 12-DIMETHYLBENZ[A]ANTHRACENE-INDUCED DAMAGE IN RAT’S (Rattus norvegicus) Liver |
| title_sort | potential of red okra abelmoschus esculentus l moench ethanol extract to protect against 7 12 dimethylbenz a anthracene induced damage in rat s rattus norvegicus liver |
| topic | antioxidant dmba healthy and affordable histopathology liver red okra ethanol extract |
| url | https://e-journal.unair.ac.id/MBIO/article/view/53448 |
| work_keys_str_mv | AT lukiteswaridyahtrihapsari potentialofredokraabelmoschusesculentuslmoenchethanolextracttoprotectagainst712dimethylbenzaanthraceneinduceddamageinratsrattusnorvegicusliver AT kusumaekopurwantari potentialofredokraabelmoschusesculentuslmoenchethanolextracttoprotectagainst712dimethylbenzaanthraceneinduceddamageinratsrattusnorvegicusliver AT indrisafitrimukono potentialofredokraabelmoschusesculentuslmoenchethanolextracttoprotectagainst712dimethylbenzaanthraceneinduceddamageinratsrattusnorvegicusliver AT sripujiastutiwahyuningsih potentialofredokraabelmoschusesculentuslmoenchethanolextracttoprotectagainst712dimethylbenzaanthraceneinduceddamageinratsrattusnorvegicusliver AT nadyatulilmaindahsavira potentialofredokraabelmoschusesculentuslmoenchethanolextracttoprotectagainst712dimethylbenzaanthraceneinduceddamageinratsrattusnorvegicusliver |