Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS
Rhizoma Anemarrhenae is a well-known herbal medicine with saponins as its commonly regarded major bioactive components. It is essential to classify the properties of saponins which are associated with their toxicity and efficacy. In this study, 25 compounds were identified by HPLC-Q-TOF/MS in the ex...
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| Format: | Article |
| Language: | English |
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Sciendo
2021-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2021-0033 |
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| author | Wang Hai-Qiao Lan Fen Zhang Yi-Han Xia Jin-Er Gong Xiao-Mei Liu Min |
| author_facet | Wang Hai-Qiao Lan Fen Zhang Yi-Han Xia Jin-Er Gong Xiao-Mei Liu Min |
| author_sort | Wang Hai-Qiao |
| collection | DOAJ |
| description | Rhizoma Anemarrhenae is a well-known herbal medicine with saponins as its commonly regarded major bioactive components. It is essential to classify the properties of saponins which are associated with their toxicity and efficacy. In this study, 25 compounds were identified by HPLC-Q-TOF/MS in the extract of Rhizoma Anemarrhenae and 8 saponins were detected in rat plasma by HPLC-MS/MS after oral administration of this extract. These were neomangiferin, mangiferin, timosaponin E1, timosaponin E, timosaponin B-II, timosaponin B-III, timosaponin A-III and timosaponin A-I. A sensitive and accurate HPLC-MS/MS method was developed and successfully applied to a pharmacokinetic study of the abovementioned eight saponins after oral administration of the Rhizoma Anemarrhenae extract to rats. The method validation, including specificity, linearity, precision, accuracy, recovery, matrix effect and robustness, met the requirements of the intended use. The pharmacokinetic parameter, Tmax value, ranged from 2 to 8 h for these eight saponins whereas their elimination half-life (t1/2) ranged from 4.06 to 9.77 h, indicating slow excretion. The plasma concentrations of these eight saponins were all very low, indicating a relatively low oral bioavailability. All these results provide support for further clinical studies. |
| first_indexed | 2024-12-16T09:04:02Z |
| format | Article |
| id | doaj.art-ce783b39ce734784816a0f8f0a8714d1 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-12-16T09:04:02Z |
| publishDate | 2021-12-01 |
| publisher | Sciendo |
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| series | Acta Pharmaceutica |
| spelling | doaj.art-ce783b39ce734784816a0f8f0a8714d12022-12-21T22:37:07ZengSciendoActa Pharmaceutica1846-95582021-12-0171456758510.2478/acph-2021-0033Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MSWang Hai-Qiao0Lan Fen1Zhang Yi-Han2Xia Jin-Er3Gong Xiao-Mei4Liu Min5Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai201112, ChinaDepartment of Pharmacy, Shanghai Changhai Hospital, Second Military Medical University, Shanghai200433, ChinaDepartment of Pharmacy, Shanghai Changhai Hospital, Second Military Medical University, Shanghai200433, ChinaDepartment of Pharmacy, Shanghai Changhai Hospital, Second Military Medical University, Shanghai200433, ChinaDepartment of Radiation Oncology, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai200433, ChinaDepartment of Pharmacy, Shanghai Changhai Hospital, Second Military Medical University, Shanghai200433, ChinaRhizoma Anemarrhenae is a well-known herbal medicine with saponins as its commonly regarded major bioactive components. It is essential to classify the properties of saponins which are associated with their toxicity and efficacy. In this study, 25 compounds were identified by HPLC-Q-TOF/MS in the extract of Rhizoma Anemarrhenae and 8 saponins were detected in rat plasma by HPLC-MS/MS after oral administration of this extract. These were neomangiferin, mangiferin, timosaponin E1, timosaponin E, timosaponin B-II, timosaponin B-III, timosaponin A-III and timosaponin A-I. A sensitive and accurate HPLC-MS/MS method was developed and successfully applied to a pharmacokinetic study of the abovementioned eight saponins after oral administration of the Rhizoma Anemarrhenae extract to rats. The method validation, including specificity, linearity, precision, accuracy, recovery, matrix effect and robustness, met the requirements of the intended use. The pharmacokinetic parameter, Tmax value, ranged from 2 to 8 h for these eight saponins whereas their elimination half-life (t1/2) ranged from 4.06 to 9.77 h, indicating slow excretion. The plasma concentrations of these eight saponins were all very low, indicating a relatively low oral bioavailability. All these results provide support for further clinical studies.https://doi.org/10.2478/acph-2021-0033rhizoma anemarrhenaesaponinidentificationpharmacokineticshplc-q-tof/mshplc-ms/ms |
| spellingShingle | Wang Hai-Qiao Lan Fen Zhang Yi-Han Xia Jin-Er Gong Xiao-Mei Liu Min Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS Acta Pharmaceutica rhizoma anemarrhenae saponin identification pharmacokinetics hplc-q-tof/ms hplc-ms/ms |
| title | Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS |
| title_full | Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS |
| title_fullStr | Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS |
| title_full_unstemmed | Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS |
| title_short | Identification and pharmacokinetics of saponins in Rhizoma Anemarrhenae after oral administration to rats by HPLC-Q-TOF/MS and HPLC-MS/MS |
| title_sort | identification and pharmacokinetics of saponins in rhizoma anemarrhenae after oral administration to rats by hplc q tof ms and hplc ms ms |
| topic | rhizoma anemarrhenae saponin identification pharmacokinetics hplc-q-tof/ms hplc-ms/ms |
| url | https://doi.org/10.2478/acph-2021-0033 |
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