Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway
Objective(s): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. Materials and Methods: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histol...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-09-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_18586_e53c599fb102d95022abf185c4628743.pdf |
| _version_ | 1819237614208679936 |
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| author | Xi Jiang Ying Hu Yingjie Zhou Jin Chen Chonglu Sun Ziwei Chen Changfeng Jing Lexing Xu Fuhe Liu Wenjuan Ni Xuefeng Yu Lei Chen |
| author_facet | Xi Jiang Ying Hu Yingjie Zhou Jin Chen Chonglu Sun Ziwei Chen Changfeng Jing Lexing Xu Fuhe Liu Wenjuan Ni Xuefeng Yu Lei Chen |
| author_sort | Xi Jiang |
| collection | DOAJ |
| description | Objective(s): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. Materials and Methods: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histological features and expression of AMPK, NF-κB, inflammatory mediators, and oxidative stress markers were compared among different groups. Results: The results showed that LPS resulted in obvious uterus damage, meanwhile, the inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α), as well as NF-κB in the uterine tissue, were significantly increased and the level of adenosine monophosphate-activated protein kinase (AMPK) was reduced. Nevertheless, pretreatment with irisin reversed the phenomena caused by LPS. Interestingly, compound C (AMPK inhibitor) abolished irisin’s effects on the uterus, which suggested that irisin’s beneficial function was achieved through regulating the AMPK-NF-κB pathway. Moreover, LPS-induced alterations of oxidative factors (MnSOD, GSH, and MDA) were reversed significantly by pretreatment with irisin. This data indicated irisin’s beneficial function was also related to antioxidation besides anti-inflammation. Conclusion: Our study implies that irisin is a potential therapeutic agent for endometritis. |
| first_indexed | 2024-12-23T13:23:08Z |
| format | Article |
| id | doaj.art-ce7a34bf6dbd4eb089d5822c52af6625 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-23T13:23:08Z |
| publishDate | 2021-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-ce7a34bf6dbd4eb089d5822c52af66252022-12-21T17:45:23ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-09-012491247125310.22038/ijbms.2021.56781.1267818586Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathwayXi Jiang0Ying Hu1Yingjie Zhou2Jin Chen3Chonglu Sun4Ziwei Chen5Changfeng Jing6Lexing Xu7Fuhe Liu8Wenjuan Ni9Xuefeng Yu10Lei Chen11Zhejiang University Mingzhou Hospital, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaZhejiang University Mingzhou Hospital, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaZhejiang University Mingzhou Hospital, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaDepartment of Pharmacy, Zhejiang Pharmaceutical College, Ningbo, 315000, ChinaObjective(s): This research was designed to determine the role of irisin in lipopolysaccharide (LPS)-induced endometritis in female mice. Materials and Methods: Animals were randomly assigned into sham, sham + irisin, LPS, LPS + irisin (0.1, 1, 10 μg/kg), and LPS + irisin + compound C groups. Histological features and expression of AMPK, NF-κB, inflammatory mediators, and oxidative stress markers were compared among different groups. Results: The results showed that LPS resulted in obvious uterus damage, meanwhile, the inflammatory mediators (COX-2, iNOS, IL-1β, IL-6, and TNF-α), as well as NF-κB in the uterine tissue, were significantly increased and the level of adenosine monophosphate-activated protein kinase (AMPK) was reduced. Nevertheless, pretreatment with irisin reversed the phenomena caused by LPS. Interestingly, compound C (AMPK inhibitor) abolished irisin’s effects on the uterus, which suggested that irisin’s beneficial function was achieved through regulating the AMPK-NF-κB pathway. Moreover, LPS-induced alterations of oxidative factors (MnSOD, GSH, and MDA) were reversed significantly by pretreatment with irisin. This data indicated irisin’s beneficial function was also related to antioxidation besides anti-inflammation. Conclusion: Our study implies that irisin is a potential therapeutic agent for endometritis.https://ijbms.mums.ac.ir/article_18586_e53c599fb102d95022abf185c4628743.pdfamp-activated protein kinasesendometritisinflammationirisinlipopolysaccharide nf-κb |
| spellingShingle | Xi Jiang Ying Hu Yingjie Zhou Jin Chen Chonglu Sun Ziwei Chen Changfeng Jing Lexing Xu Fuhe Liu Wenjuan Ni Xuefeng Yu Lei Chen Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway Iranian Journal of Basic Medical Sciences amp-activated protein kinases endometritis inflammation irisin lipopolysaccharide nf-κb |
| title | Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway |
| title_full | Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway |
| title_fullStr | Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway |
| title_full_unstemmed | Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway |
| title_short | Irisin protects female mice with LPS-induced endometritis through the AMPK/NF-κB pathway |
| title_sort | irisin protects female mice with lps induced endometritis through the ampk nf κb pathway |
| topic | amp-activated protein kinases endometritis inflammation irisin lipopolysaccharide nf-κb |
| url | https://ijbms.mums.ac.ir/article_18586_e53c599fb102d95022abf185c4628743.pdf |
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