Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination
Retrovirus population diversity within infected hosts is commonly high due in part to elevated rates of replication, mutation, and recombination. This high genetic diversity often complicates the development of effective diagnostics, vaccines, and antiviral drugs. This review highlights the diverse...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-09-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/6/9/3612 |
| _version_ | 1818172789673164800 |
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| author | Jonathan M.O. Rawson Louis M. Mansky |
| author_facet | Jonathan M.O. Rawson Louis M. Mansky |
| author_sort | Jonathan M.O. Rawson |
| collection | DOAJ |
| description | Retrovirus population diversity within infected hosts is commonly high due in part to elevated rates of replication, mutation, and recombination. This high genetic diversity often complicates the development of effective diagnostics, vaccines, and antiviral drugs. This review highlights the diverse vectors and approaches that have been used to examine mutation and recombination in retroviruses. Retroviral vectors for these purposes can broadly be divided into two categories: those that utilize reporter genes as mutation or recombination targets and those that utilize viral genes as targets of mutation or recombination. Reporter gene vectors greatly facilitate the detection, quantification, and characterization of mutants and/or recombinants, but may not fully recapitulate the patterns of mutagenesis or recombination observed in native viral gene sequences. In contrast, the detection of mutations or recombination events directly in viral genes is more biologically relevant but also typically more challenging and inefficient. We will highlight the advantages and disadvantages of the various vectors and approaches used as well as propose ways in which they could be improved. |
| first_indexed | 2024-12-11T19:18:12Z |
| format | Article |
| id | doaj.art-ce7af3cafc47429b8184ac9106302c51 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-11T19:18:12Z |
| publishDate | 2014-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-ce7af3cafc47429b8184ac9106302c512022-12-22T00:53:36ZengMDPI AGViruses1999-49152014-09-01693612364210.3390/v6093612v6093612Retroviral Vectors for Analysis of Viral Mutagenesis and RecombinationJonathan M.O. Rawson0Louis M. Mansky1Institute for Molecular Virology, University of Minnesota, Moos Tower 18-242, 515 Delaware St SE, Minneapolis, MN 55455, USAInstitute for Molecular Virology, University of Minnesota, Moos Tower 18-242, 515 Delaware St SE, Minneapolis, MN 55455, USARetrovirus population diversity within infected hosts is commonly high due in part to elevated rates of replication, mutation, and recombination. This high genetic diversity often complicates the development of effective diagnostics, vaccines, and antiviral drugs. This review highlights the diverse vectors and approaches that have been used to examine mutation and recombination in retroviruses. Retroviral vectors for these purposes can broadly be divided into two categories: those that utilize reporter genes as mutation or recombination targets and those that utilize viral genes as targets of mutation or recombination. Reporter gene vectors greatly facilitate the detection, quantification, and characterization of mutants and/or recombinants, but may not fully recapitulate the patterns of mutagenesis or recombination observed in native viral gene sequences. In contrast, the detection of mutations or recombination events directly in viral genes is more biologically relevant but also typically more challenging and inefficient. We will highlight the advantages and disadvantages of the various vectors and approaches used as well as propose ways in which they could be improved.http://www.mdpi.com/1999-4915/6/9/3612retroviruslentivirusreverse transcriptionevolutionmutationrecombinationretroviral vector |
| spellingShingle | Jonathan M.O. Rawson Louis M. Mansky Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination Viruses retrovirus lentivirus reverse transcription evolution mutation recombination retroviral vector |
| title | Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination |
| title_full | Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination |
| title_fullStr | Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination |
| title_full_unstemmed | Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination |
| title_short | Retroviral Vectors for Analysis of Viral Mutagenesis and Recombination |
| title_sort | retroviral vectors for analysis of viral mutagenesis and recombination |
| topic | retrovirus lentivirus reverse transcription evolution mutation recombination retroviral vector |
| url | http://www.mdpi.com/1999-4915/6/9/3612 |
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