Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles
Purpose: Today, the discovery of novel and effective chemotherapeutic compounds is the mainchallenge in cancer therapy. In recent years, the anti-tumoral activity of natural naphthoquinonejuglone (JUG), present in different parts of walnut trees, has received considerable interest. Thepurpose of the...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2022-08-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | https://apb.tbzmed.ac.ir/PDF/apb-12-818.pdf |
| _version_ | 1811269764918542336 |
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| author | Ali Jahanban-Esfahlan Soodabeh Davaran Siavoush Dastmalchi |
| author_facet | Ali Jahanban-Esfahlan Soodabeh Davaran Siavoush Dastmalchi |
| author_sort | Ali Jahanban-Esfahlan |
| collection | DOAJ |
| description | Purpose: Today, the discovery of novel and effective chemotherapeutic compounds is the mainchallenge in cancer therapy. In recent years, the anti-tumoral activity of natural naphthoquinonejuglone (JUG), present in different parts of walnut trees, has received considerable interest. Thepurpose of the current study was to prepare and evaluate the in vitro antiproliferative activity ofJUG-loaded bovine serum albumin nanoparticles (JUG-BSA NPs).Methods: BSA NPs and JUG-BSA NPs were prepared using the desolvation technique. The NPswere characterized for their particle size (PS), zeta potential (ZP), drug loading (DL) capacityand encapsulation efficiency (EE). The anti-proliferative activity of JUG-BSA NPs was evaluatedon A431 and HT29 cancer cell lines using cellular uptake and MTT assays.Results: The PS and ZP values of JUG-BSA NPs were 85 ± 6.55 nm and −29.6 mV, respectively.The DL capacity and EE were 3.7% to 5% and 50.4% to 94.6%, respectively. The cytotoxicityof JUG-BSA NPs was significantly less on both cultured A431 and HT29 cells at the studiedconcentrations when compared to free JUG. However, the effect was not very substantial,particularly at high levels.Conclusion: In conclusion, BSA NPs can be used as a suitable and safe carrier for the deliveryof JUG, a cytotoxic hydrophobic natural compound. |
| first_indexed | 2024-04-12T21:49:04Z |
| format | Article |
| id | doaj.art-ce7d0caee97d4a539ea5f96a8f637523 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-04-12T21:49:04Z |
| publishDate | 2022-08-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-ce7d0caee97d4a539ea5f96a8f6375232022-12-22T03:15:33ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082022-08-0112481882710.34172/apb.2022.087apb-28658Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA NanoparticlesAli Jahanban-Esfahlan0Soodabeh Davaran1Siavoush Dastmalchi2Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Purpose: Today, the discovery of novel and effective chemotherapeutic compounds is the mainchallenge in cancer therapy. In recent years, the anti-tumoral activity of natural naphthoquinonejuglone (JUG), present in different parts of walnut trees, has received considerable interest. Thepurpose of the current study was to prepare and evaluate the in vitro antiproliferative activity ofJUG-loaded bovine serum albumin nanoparticles (JUG-BSA NPs).Methods: BSA NPs and JUG-BSA NPs were prepared using the desolvation technique. The NPswere characterized for their particle size (PS), zeta potential (ZP), drug loading (DL) capacityand encapsulation efficiency (EE). The anti-proliferative activity of JUG-BSA NPs was evaluatedon A431 and HT29 cancer cell lines using cellular uptake and MTT assays.Results: The PS and ZP values of JUG-BSA NPs were 85 ± 6.55 nm and −29.6 mV, respectively.The DL capacity and EE were 3.7% to 5% and 50.4% to 94.6%, respectively. The cytotoxicityof JUG-BSA NPs was significantly less on both cultured A431 and HT29 cells at the studiedconcentrations when compared to free JUG. However, the effect was not very substantial,particularly at high levels.Conclusion: In conclusion, BSA NPs can be used as a suitable and safe carrier for the deliveryof JUG, a cytotoxic hydrophobic natural compound.https://apb.tbzmed.ac.ir/PDF/apb-12-818.pdfbsacancerchemotherapydrug deliveryjuglonenanocarriersprotein |
| spellingShingle | Ali Jahanban-Esfahlan Soodabeh Davaran Siavoush Dastmalchi Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles Advanced Pharmaceutical Bulletin bsa cancer chemotherapy drug delivery juglone nanocarriers protein |
| title | Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles |
| title_full | Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles |
| title_fullStr | Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles |
| title_full_unstemmed | Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles |
| title_short | Preparation and Antiproliferative Activity Evaluation of Juglone-Loaded BSA Nanoparticles |
| title_sort | preparation and antiproliferative activity evaluation of juglone loaded bsa nanoparticles |
| topic | bsa cancer chemotherapy drug delivery juglone nanocarriers protein |
| url | https://apb.tbzmed.ac.ir/PDF/apb-12-818.pdf |
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