Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites

Differences in the efficacy of diagnostic techniques employed in the parasitological examination of feces are a limiting factor of this laboratory procedure in the field of Veterinary Parasitology. To verify advances in this type of examination in dogs, we conducted a study using a new technique (TF...

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Main Authors: Willian Marinho Dourado Coelho, Jancarlo Ferreira Gomes, Alexandre Xavier Falcão, Bianca Martins dos Santos, Felipe Augusto Soares, Celso Tetsuo Nagase Suzuki, Alessandro Francisco Talamini do Amarante, Katia Denise Saraiva Bresciani
Format: Article
Language:English
Published: Colégio Brasileiro de Parasitologia Veterinaria
Series:Revista Brasileira de Parasitologia Veterinária
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200223&lng=en&tlng=en
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author Willian Marinho Dourado Coelho
Jancarlo Ferreira Gomes
Alexandre Xavier Falcão
Bianca Martins dos Santos
Felipe Augusto Soares
Celso Tetsuo Nagase Suzuki
Alessandro Francisco Talamini do Amarante
Katia Denise Saraiva Bresciani
author_facet Willian Marinho Dourado Coelho
Jancarlo Ferreira Gomes
Alexandre Xavier Falcão
Bianca Martins dos Santos
Felipe Augusto Soares
Celso Tetsuo Nagase Suzuki
Alessandro Francisco Talamini do Amarante
Katia Denise Saraiva Bresciani
author_sort Willian Marinho Dourado Coelho
collection DOAJ
description Differences in the efficacy of diagnostic techniques employed in the parasitological examination of feces are a limiting factor of this laboratory procedure in the field of Veterinary Parasitology. To verify advances in this type of examination in dogs, we conducted a study using a new technique (TFGII/Dog). Fifty naturally infected dogs were housed in individual stalls, and their feces were evaluated comparatively using this technique and four other conventional techniques. The TFGII/Dog showed high levels of sensitivity and efficiency, surpassing the diagnostic accuracy of the other techniques with a kappa concordance index of 0.739 (Substantial), as opposed to 0.546 (Moderate), 0.485 (Moderate), 0.467 (Moderate), and 0.325 (Fair) of the Spontaneous-Sedimentation, Centrifugal-Flotation in Saturated Zinc Sulfate Solution, Centrifugal-Flotation in Saturated Sugar Solution, and Spontaneous-Flotation in Saturated Sodium Chloride Solution techniques, respectively. The combination of positive results of all techniques comprises eight genera of parasites, with Ancylostoma spp. predominating among helminths, and Cystoisospora spp. among protozoa. The TFGII/Dog technique showed better diagnostic performance, and can therefore be considered an important tool for optimizing the results of laboratory routines and for the control of canine gastrointestinal parasites.
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spelling doaj.art-ce8041e2327644b0b0eb0ac38e692a202022-12-22T01:39:40ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296124222322610.1590/S1984-29612015032S1984-29612015000200223Comparative study of five techniques for the diagnosis of canine gastrointestinal parasitesWillian Marinho Dourado CoelhoJancarlo Ferreira GomesAlexandre Xavier FalcãoBianca Martins dos SantosFelipe Augusto SoaresCelso Tetsuo Nagase SuzukiAlessandro Francisco Talamini do AmaranteKatia Denise Saraiva BrescianiDifferences in the efficacy of diagnostic techniques employed in the parasitological examination of feces are a limiting factor of this laboratory procedure in the field of Veterinary Parasitology. To verify advances in this type of examination in dogs, we conducted a study using a new technique (TFGII/Dog). Fifty naturally infected dogs were housed in individual stalls, and their feces were evaluated comparatively using this technique and four other conventional techniques. The TFGII/Dog showed high levels of sensitivity and efficiency, surpassing the diagnostic accuracy of the other techniques with a kappa concordance index of 0.739 (Substantial), as opposed to 0.546 (Moderate), 0.485 (Moderate), 0.467 (Moderate), and 0.325 (Fair) of the Spontaneous-Sedimentation, Centrifugal-Flotation in Saturated Zinc Sulfate Solution, Centrifugal-Flotation in Saturated Sugar Solution, and Spontaneous-Flotation in Saturated Sodium Chloride Solution techniques, respectively. The combination of positive results of all techniques comprises eight genera of parasites, with Ancylostoma spp. predominating among helminths, and Cystoisospora spp. among protozoa. The TFGII/Dog technique showed better diagnostic performance, and can therefore be considered an important tool for optimizing the results of laboratory routines and for the control of canine gastrointestinal parasites.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200223&lng=en&tlng=enParasitological techniquesdoghelminthsprotozoaTFGII/Dog
spellingShingle Willian Marinho Dourado Coelho
Jancarlo Ferreira Gomes
Alexandre Xavier Falcão
Bianca Martins dos Santos
Felipe Augusto Soares
Celso Tetsuo Nagase Suzuki
Alessandro Francisco Talamini do Amarante
Katia Denise Saraiva Bresciani
Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites
Revista Brasileira de Parasitologia Veterinária
Parasitological techniques
dog
helminths
protozoa
TFGII/Dog
title Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites
title_full Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites
title_fullStr Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites
title_full_unstemmed Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites
title_short Comparative study of five techniques for the diagnosis of canine gastrointestinal parasites
title_sort comparative study of five techniques for the diagnosis of canine gastrointestinal parasites
topic Parasitological techniques
dog
helminths
protozoa
TFGII/Dog
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612015000200223&lng=en&tlng=en
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