Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it>
<p>Abstract</p> <p>Background</p> <p>Dupuytren's contracture or disease (DD) is a fibro-proliferative disease of the hand that results in the development of scar-like, collagen-rich disease cords within specific palmar fascia bands. Although the molecular pathology...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2003-07-01
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| Series: | BMC Musculoskeletal Disorders |
| Online Access: | http://www.biomedcentral.com/1471-2474/4/16 |
| _version_ | 1818485015767416832 |
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| author | Varallo Vincenzo M Howard Jeffrey C Ross Douglas C Roth James H Faber Kenneth J Alman Benjamin Gan Bing |
| author_facet | Varallo Vincenzo M Howard Jeffrey C Ross Douglas C Roth James H Faber Kenneth J Alman Benjamin Gan Bing |
| author_sort | Varallo Vincenzo M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Dupuytren's contracture or disease (DD) is a fibro-proliferative disease of the hand that results in the development of scar-like, collagen-rich disease cords within specific palmar fascia bands. Although the molecular pathology of DD is unknown, recent evidence suggests that β-catenin may play a role. In this study, collagen matrix cultures of primary disease fibroblasts show enhanced contraction and isometric tension-dependent changes in β-catenin and fibronectin levels.</p> <p>Methods</p> <p>Western blots of β-catenin and fibronectin levels were determined for control and disease primary cell cultures grown within stressed- and attached-collagen matrices. Collagen contraction was quantified, and immunocytochemistry analysis of filamentous actin performed.</p> <p>Results</p> <p>Disease cells exhibited enhanced collagen contraction activity compared to control cells. Alterations in isometric tension of collagen matrices triggered dramatic changes in β-catenin and fibronectin levels, including a transient increase in β-catenin levels within disease cells, while fibronectin levels steadily decreased to levels below those seen in normal cell cultures. In contrast, both fibronectin and β-catenin levels increased in attached collagen-matrix cultures of disease cells, while control cultures showed only increases in fibronectin levels. Immunocytochemistry analysis also revealed extensive filamentous actin networks in disease cells, and enhanced attachment and spreading of disease cell in collagen matrices.</p> <p>Conclusion</p> <p>Three-dimensional collagen matrix cultures of primary disease cell lines are more contractile and express a more extensive filamentous actin network than patient-matched control cultures. The elevated levels of β-catenin and Fn seen in collagen matrix cultures of disease fibroblasts can be regulated by changes in isometric tension.</p> |
| first_indexed | 2024-12-10T16:02:30Z |
| format | Article |
| id | doaj.art-ce83350a4ea04fbc925c7532c4064d34 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-10T16:02:30Z |
| publishDate | 2003-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-ce83350a4ea04fbc925c7532c4064d342022-12-22T01:42:22ZengBMCBMC Musculoskeletal Disorders1471-24742003-07-01411610.1186/1471-2474-4-16Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it>Varallo Vincenzo MHoward Jeffrey CRoss Douglas CRoth James HFaber Kenneth JAlman BenjaminGan Bing<p>Abstract</p> <p>Background</p> <p>Dupuytren's contracture or disease (DD) is a fibro-proliferative disease of the hand that results in the development of scar-like, collagen-rich disease cords within specific palmar fascia bands. Although the molecular pathology of DD is unknown, recent evidence suggests that β-catenin may play a role. In this study, collagen matrix cultures of primary disease fibroblasts show enhanced contraction and isometric tension-dependent changes in β-catenin and fibronectin levels.</p> <p>Methods</p> <p>Western blots of β-catenin and fibronectin levels were determined for control and disease primary cell cultures grown within stressed- and attached-collagen matrices. Collagen contraction was quantified, and immunocytochemistry analysis of filamentous actin performed.</p> <p>Results</p> <p>Disease cells exhibited enhanced collagen contraction activity compared to control cells. Alterations in isometric tension of collagen matrices triggered dramatic changes in β-catenin and fibronectin levels, including a transient increase in β-catenin levels within disease cells, while fibronectin levels steadily decreased to levels below those seen in normal cell cultures. In contrast, both fibronectin and β-catenin levels increased in attached collagen-matrix cultures of disease cells, while control cultures showed only increases in fibronectin levels. Immunocytochemistry analysis also revealed extensive filamentous actin networks in disease cells, and enhanced attachment and spreading of disease cell in collagen matrices.</p> <p>Conclusion</p> <p>Three-dimensional collagen matrix cultures of primary disease cell lines are more contractile and express a more extensive filamentous actin network than patient-matched control cultures. The elevated levels of β-catenin and Fn seen in collagen matrix cultures of disease fibroblasts can be regulated by changes in isometric tension.</p>http://www.biomedcentral.com/1471-2474/4/16 |
| spellingShingle | Varallo Vincenzo M Howard Jeffrey C Ross Douglas C Roth James H Faber Kenneth J Alman Benjamin Gan Bing Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it> BMC Musculoskeletal Disorders |
| title | Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it> |
| title_full | Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it> |
| title_fullStr | Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it> |
| title_full_unstemmed | Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it> |
| title_short | Elevated levels of β-catenin and fibronectin in three-dimensional collagen cultures of Dupuytren's disease cells are regulated by tension <it>in vitro</it> |
| title_sort | elevated levels of β catenin and fibronectin in three dimensional collagen cultures of dupuytren s disease cells are regulated by tension it in vitro it |
| url | http://www.biomedcentral.com/1471-2474/4/16 |
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