Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/Nanoparticles
Coronavirus disease 2019 (COVID-19) has caused a global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The viral infection is reliant upon the binding between angiotensin-converting enzyme 2 receptor (ACE2) and spike protein (S). Therefore, ACE2 is a key receptor for SARS-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/6/652 |
| _version_ | 1797596054639607808 |
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| author | Soha Y. Alkhaldi Ian Peng Ching-An Peng |
| author_facet | Soha Y. Alkhaldi Ian Peng Ching-An Peng |
| author_sort | Soha Y. Alkhaldi |
| collection | DOAJ |
| description | Coronavirus disease 2019 (COVID-19) has caused a global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The viral infection is reliant upon the binding between angiotensin-converting enzyme 2 receptor (ACE2) and spike protein (S). Therefore, ACE2 is a key receptor for SARS-CoV-2 to infect the host. Nonetheless, as SARS-CoV-2 is constantly mutating into new variants that cause high infection rates, the development of prophylactic and therapeutic approaches remains a necessity to continue fighting mutated SARS-CoV-2 variants. In this study, ACE2-streptavidin fusion proteins expressed by recombinant DNA technology were anchored on biotinylated fluorescent polystyrene particles of various sizes ranging from 0.15 to 5 µm. The ACE2-tethered micro/nanoparticles were shown to prevent spike protein pseudotyped lentivirus entry into ACE2-expressing HEK293T cells. Compared to ACE2 in soluble form, micro-sized particles (2 and 5 µm) immobilized with ACE2 interfered more efficiently with viral attachment, entry, and the ensuing infection. Our results showed that particles functionalized with ACE2 could be used as efficient decoys to block the infection of SARS-CoV-2 strains. |
| first_indexed | 2024-03-11T02:46:06Z |
| format | Article |
| id | doaj.art-ce845b7fc9584674a323aef76659143c |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T02:46:06Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-ce845b7fc9584674a323aef76659143c2023-11-18T09:20:53ZengMDPI AGBioengineering2306-53542023-05-0110665210.3390/bioengineering10060652Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/NanoparticlesSoha Y. Alkhaldi0Ian Peng1Ching-An Peng2Department of Chemical and Biological Engineering, University of Idaho, Moscow, ID 83844, USADepartment of Chemical and Biological Engineering, University of Idaho, Moscow, ID 83844, USADepartment of Chemical and Biological Engineering, University of Idaho, Moscow, ID 83844, USACoronavirus disease 2019 (COVID-19) has caused a global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The viral infection is reliant upon the binding between angiotensin-converting enzyme 2 receptor (ACE2) and spike protein (S). Therefore, ACE2 is a key receptor for SARS-CoV-2 to infect the host. Nonetheless, as SARS-CoV-2 is constantly mutating into new variants that cause high infection rates, the development of prophylactic and therapeutic approaches remains a necessity to continue fighting mutated SARS-CoV-2 variants. In this study, ACE2-streptavidin fusion proteins expressed by recombinant DNA technology were anchored on biotinylated fluorescent polystyrene particles of various sizes ranging from 0.15 to 5 µm. The ACE2-tethered micro/nanoparticles were shown to prevent spike protein pseudotyped lentivirus entry into ACE2-expressing HEK293T cells. Compared to ACE2 in soluble form, micro-sized particles (2 and 5 µm) immobilized with ACE2 interfered more efficiently with viral attachment, entry, and the ensuing infection. Our results showed that particles functionalized with ACE2 could be used as efficient decoys to block the infection of SARS-CoV-2 strains.https://www.mdpi.com/2306-5354/10/6/652SARS-CoV-2ACE2spike proteinpseudotyped lentivirusfunctionalized particlescore streptavidin |
| spellingShingle | Soha Y. Alkhaldi Ian Peng Ching-An Peng Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/Nanoparticles Bioengineering SARS-CoV-2 ACE2 spike protein pseudotyped lentivirus functionalized particles core streptavidin |
| title | Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/Nanoparticles |
| title_full | Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/Nanoparticles |
| title_fullStr | Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/Nanoparticles |
| title_full_unstemmed | Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/Nanoparticles |
| title_short | Inhibition of SARS-CoV-2 Spike Protein Pseudotyped Virus Infection Using ACE2-Tethered Micro/Nanoparticles |
| title_sort | inhibition of sars cov 2 spike protein pseudotyped virus infection using ace2 tethered micro nanoparticles |
| topic | SARS-CoV-2 ACE2 spike protein pseudotyped lentivirus functionalized particles core streptavidin |
| url | https://www.mdpi.com/2306-5354/10/6/652 |
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