Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disorders
Asthenozoospermia is the most frequent sperm motility disorder, but there are other more extreme sperm motility disorders, namely oligo-astheno-teratozoospermia (OAT) and necrozoospermia. There are several cellular mechanisms known for OAT and necrozoospermia, but there are limited data on dynein AT...
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| Language: | English |
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PAGEPress Publications
2019-09-01
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| Series: | Journal of Biological Research |
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| Online Access: | https://www.pagepressjournals.org/index.php/jbr/article/view/8277 |
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| author | Silvia W. Lestari Fitrah Firdaus Dessy Noor Miati - Asmarinah |
| author_facet | Silvia W. Lestari Fitrah Firdaus Dessy Noor Miati - Asmarinah |
| author_sort | Silvia W. Lestari |
| collection | DOAJ |
| description | Asthenozoospermia is the most frequent sperm motility disorder, but there are other more extreme sperm motility disorders, namely oligo-astheno-teratozoospermia (OAT) and necrozoospermia. There are several cellular mechanisms known for OAT and necrozoospermia, but there are limited data on dynein ATPase and ATPases associated with various cellular activities (AAA+). AAA1 is involved in ATP hydrolysis, while AAA2 is entangled in ATP-binding pocket. This study was conducted to investigate the role of dynein ATPase activity and quantification of AAA dynein. Spermatozoa from 14 men with OAT, 11 men with necrozoospermic and 17 men with normoozspermic samples were used in this study. Makler chamber was used to determine sperm concentration and motility, while Papanicolaou stained semen smears using World Health Organization-fifth edition criteria was performed to determine sperm morphology, and dynein ATPase was quantified by calculation of released inorganic phosphate. AAA was quantified by enzyme-linked immunosorbent assay, whereas the distribution was determined by immunocytochemistry. This study showed that the dynein ATPase activity in OAT and necrozoospermia was lower than in the normozoospermic group (2.68±0.76, 1.01±0.31, 7.22±1.08 μmol Pi/mg protein/h, respectively, P<0.05), as well as the amounts of AAA1 and AAA2. In addition, staining for AAA in the sperm tail paralleled the dynein ATPase activity and quantity of AAA, being the highest in sperm from normozoospermic samples, lower in sperm from OAT samples, and almost undetectable in sperm from necrozoospermic samples. The structure and function of damaged sperm dynein may alter dynein ATPase activity and levels of AAA1 and AAA2. |
| first_indexed | 2024-04-12T21:06:12Z |
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| institution | Directory Open Access Journal |
| issn | 1826-8838 2284-0230 |
| language | English |
| last_indexed | 2024-04-12T21:06:12Z |
| publishDate | 2019-09-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Journal of Biological Research |
| spelling | doaj.art-ce85e54966d64a43877698d99018b8fc2022-12-22T03:16:41ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302019-09-0192210.4081/jbr.2019.8277Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disordersSilvia W. Lestari0Fitrah Firdaus1Dessy Noor Miati2- Asmarinah3Department of Medical Biology, Faculty of Medicine, University of Indonesia, JakartaMaster Program in Biomedical Sciences, Faculty of Medicine, University of Indonesia, JakartaMaster Program in Biomedical Sciences, Faculty of Medicine, University of Indonesia, JakartaDepartment of Medical Biology, Faculty of Medicine, University of Indonesia, JakartaAsthenozoospermia is the most frequent sperm motility disorder, but there are other more extreme sperm motility disorders, namely oligo-astheno-teratozoospermia (OAT) and necrozoospermia. There are several cellular mechanisms known for OAT and necrozoospermia, but there are limited data on dynein ATPase and ATPases associated with various cellular activities (AAA+). AAA1 is involved in ATP hydrolysis, while AAA2 is entangled in ATP-binding pocket. This study was conducted to investigate the role of dynein ATPase activity and quantification of AAA dynein. Spermatozoa from 14 men with OAT, 11 men with necrozoospermic and 17 men with normoozspermic samples were used in this study. Makler chamber was used to determine sperm concentration and motility, while Papanicolaou stained semen smears using World Health Organization-fifth edition criteria was performed to determine sperm morphology, and dynein ATPase was quantified by calculation of released inorganic phosphate. AAA was quantified by enzyme-linked immunosorbent assay, whereas the distribution was determined by immunocytochemistry. This study showed that the dynein ATPase activity in OAT and necrozoospermia was lower than in the normozoospermic group (2.68±0.76, 1.01±0.31, 7.22±1.08 μmol Pi/mg protein/h, respectively, P<0.05), as well as the amounts of AAA1 and AAA2. In addition, staining for AAA in the sperm tail paralleled the dynein ATPase activity and quantity of AAA, being the highest in sperm from normozoospermic samples, lower in sperm from OAT samples, and almost undetectable in sperm from necrozoospermic samples. The structure and function of damaged sperm dynein may alter dynein ATPase activity and levels of AAA1 and AAA2.https://www.pagepressjournals.org/index.php/jbr/article/view/8277Dynein ATPaseOligo-astheno-teratozoospermiaNecrozoospermiaSperm motility disorder |
| spellingShingle | Silvia W. Lestari Fitrah Firdaus Dessy Noor Miati - Asmarinah Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disorders Journal of Biological Research Dynein ATPase Oligo-astheno-teratozoospermia Necrozoospermia Sperm motility disorder |
| title | Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disorders |
| title_full | Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disorders |
| title_fullStr | Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disorders |
| title_full_unstemmed | Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disorders |
| title_short | Sperm dynein ATPase and ATPases associated with various cellular activities (AAA+): regulation in oligo-astheno-teratozoospermia and necrozoospermia as extreme sperm motility disorders |
| title_sort | sperm dynein atpase and atpases associated with various cellular activities aaa regulation in oligo astheno teratozoospermia and necrozoospermia as extreme sperm motility disorders |
| topic | Dynein ATPase Oligo-astheno-teratozoospermia Necrozoospermia Sperm motility disorder |
| url | https://www.pagepressjournals.org/index.php/jbr/article/view/8277 |
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