Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro
Abstract Background Porcine epidemic diarrhea virus (PEDV) of the family Coronaviridae has caused substantial economic losses in the swine husbandry industry. There’s currently no specific drug available for treatment of coronaviruses or PEDV. Method In the current study, we use coronavirus PEDV as...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-06-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12985-020-01342-w |
| _version_ | 1818340667853635584 |
|---|---|
| author | Cui-Cui Li Xiao-Jia Wang |
| author_facet | Cui-Cui Li Xiao-Jia Wang |
| author_sort | Cui-Cui Li |
| collection | DOAJ |
| description | Abstract Background Porcine epidemic diarrhea virus (PEDV) of the family Coronaviridae has caused substantial economic losses in the swine husbandry industry. There’s currently no specific drug available for treatment of coronaviruses or PEDV. Method In the current study, we use coronavirus PEDV as a model to study antiviral agents. Briefly, a fusion inhibitor tHR2, recombinant lentivirus-delivered shRNAs targeted to conserved M and N sequences, homoharringtonine (HHT), and hydroxychloroquine (HCQ) were surveyed for their antiviral effects. Results Treatment with HCQ at 50 μM and HHT at 150 nM reduced virus titer in TCID50 by 30 and 3.5 fold respectively, and the combination reduced virus titer in TCID50 by 200 fold. Conclusion Our report demonstrates that the combination of HHT and HCQ exhibited higher antiviral activity than either HHT or HCQ exhibited. The information may contribute to the development of antiviral strategies effective in controlling PEDV infection. |
| first_indexed | 2024-12-13T15:46:33Z |
| format | Article |
| id | doaj.art-ce877d3f2efc461581e84a023b067bb3 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-12-13T15:46:33Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-ce877d3f2efc461581e84a023b067bb32022-12-21T23:39:42ZengBMCVirology Journal1743-422X2020-06-0117111110.1186/s12985-020-01342-wThree kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitroCui-Cui Li0Xiao-Jia Wang1Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural UniversityKey Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural UniversityAbstract Background Porcine epidemic diarrhea virus (PEDV) of the family Coronaviridae has caused substantial economic losses in the swine husbandry industry. There’s currently no specific drug available for treatment of coronaviruses or PEDV. Method In the current study, we use coronavirus PEDV as a model to study antiviral agents. Briefly, a fusion inhibitor tHR2, recombinant lentivirus-delivered shRNAs targeted to conserved M and N sequences, homoharringtonine (HHT), and hydroxychloroquine (HCQ) were surveyed for their antiviral effects. Results Treatment with HCQ at 50 μM and HHT at 150 nM reduced virus titer in TCID50 by 30 and 3.5 fold respectively, and the combination reduced virus titer in TCID50 by 200 fold. Conclusion Our report demonstrates that the combination of HHT and HCQ exhibited higher antiviral activity than either HHT or HCQ exhibited. The information may contribute to the development of antiviral strategies effective in controlling PEDV infection.http://link.springer.com/article/10.1186/s12985-020-01342-wCoronavirusPEDVShort hairpin RNAHeptad repeatHomoharringtonineHydroxychloroquine |
| spellingShingle | Cui-Cui Li Xiao-Jia Wang Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro Virology Journal Coronavirus PEDV Short hairpin RNA Heptad repeat Homoharringtonine Hydroxychloroquine |
| title | Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro |
| title_full | Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro |
| title_fullStr | Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro |
| title_full_unstemmed | Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro |
| title_short | Three kinds of treatment with Homoharringtonine, Hydroxychloroquine or shRNA and their combination against coronavirus PEDV in vitro |
| title_sort | three kinds of treatment with homoharringtonine hydroxychloroquine or shrna and their combination against coronavirus pedv in vitro |
| topic | Coronavirus PEDV Short hairpin RNA Heptad repeat Homoharringtonine Hydroxychloroquine |
| url | http://link.springer.com/article/10.1186/s12985-020-01342-w |
| work_keys_str_mv | AT cuicuili threekindsoftreatmentwithhomoharringtoninehydroxychloroquineorshrnaandtheircombinationagainstcoronaviruspedvinvitro AT xiaojiawang threekindsoftreatmentwithhomoharringtoninehydroxychloroquineorshrnaandtheircombinationagainstcoronaviruspedvinvitro |