Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage Seeds
<i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) is an important seed-borne bacterial pathogen that causes black rot in brassica. Current seed disinfection methods for <i>Xcc</i> have disadvantages; chemical treatment has associated env...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1606 |
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| author | Akikazu Sakudo Makoto Haritani Koichi Furusaki Rumiko Onishi Takashi Onodera |
| author_facet | Akikazu Sakudo Makoto Haritani Koichi Furusaki Rumiko Onishi Takashi Onodera |
| author_sort | Akikazu Sakudo |
| collection | DOAJ |
| description | <i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) is an important seed-borne bacterial pathogen that causes black rot in brassica. Current seed disinfection methods for <i>Xcc</i> have disadvantages; chemical treatment has associated environmental risks, hot water immersion reduces germination, and dry heat treatment is protracted. Here, we treated <i>Xcc</i>-contaminated seeds with CAC-717, a recently developed disinfectant produced by applying an electric field and water flow to distilled water containing calcium hydrogen carbonate to produce mesoscopic crystals. The decimal reduction time (<i>D</i>-value) of <i>Xcc</i> suspension (8.22 log<sub>10</sub> colony forming units (CFU)/mL) by CAC-717 treatment was 0.319 min. Treatment of <i>Xcc</i>-contaminated cabbage seeds at 25 °C for 30 min with CAC-717 significantly reduced bacterial cell numbers recovered from the seeds (0.36 log<sub>10</sub> CFU/mL (SEM (standard error of the mean) = 0.23 log<sub>10</sub> CFU/mL)) compared with distilled water treatment (3.52 log<sub>10</sub> CFU/mL (SEM = 0.12 log<sub>10</sub> CFU/mL)). Moreover, there was a lower incidence of black rot after treatment with CAC-717 (26.67% ± 3.33%) versus distilled water (56.67% ± 8.82%). For non-contaminated seeds, there was no significant difference in germination rate and plant stem length between distilled water and CAC-717 treatment after 5 days of cultivation. In conclusion, CAC-717 is a promising seed disinfectant without deleterious effects on germination or plant growth. |
| first_indexed | 2024-03-10T15:30:55Z |
| format | Article |
| id | doaj.art-ce89bab2659b43a4bcd593679e27d6c9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:30:55Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ce89bab2659b43a4bcd593679e27d6c92023-11-20T17:41:56ZengMDPI AGMicroorganisms2076-26072020-10-01810160610.3390/microorganisms8101606Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage SeedsAkikazu Sakudo0Makoto Haritani1Koichi Furusaki2Rumiko Onishi3Takashi Onodera4School of Veterinary Medicine, Okayama University of Science, Imabari, Ehime 794-8555, JapanLaboratory of Environmental Science for Sustainable Development, Department of Global Agricultural Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, JapanMineral Activation Technical Research Center, Omuta, Fukuoka 836-0041, JapanSanta Mineral Co., Ltd., Minato-ku, Tokyo 105-0013, JapanLaboratory of Environmental Science for Sustainable Development, Department of Global Agricultural Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan<i>Xanthomonas campestris</i> pv. <i>campestris</i> (<i>Xcc</i>) is an important seed-borne bacterial pathogen that causes black rot in brassica. Current seed disinfection methods for <i>Xcc</i> have disadvantages; chemical treatment has associated environmental risks, hot water immersion reduces germination, and dry heat treatment is protracted. Here, we treated <i>Xcc</i>-contaminated seeds with CAC-717, a recently developed disinfectant produced by applying an electric field and water flow to distilled water containing calcium hydrogen carbonate to produce mesoscopic crystals. The decimal reduction time (<i>D</i>-value) of <i>Xcc</i> suspension (8.22 log<sub>10</sub> colony forming units (CFU)/mL) by CAC-717 treatment was 0.319 min. Treatment of <i>Xcc</i>-contaminated cabbage seeds at 25 °C for 30 min with CAC-717 significantly reduced bacterial cell numbers recovered from the seeds (0.36 log<sub>10</sub> CFU/mL (SEM (standard error of the mean) = 0.23 log<sub>10</sub> CFU/mL)) compared with distilled water treatment (3.52 log<sub>10</sub> CFU/mL (SEM = 0.12 log<sub>10</sub> CFU/mL)). Moreover, there was a lower incidence of black rot after treatment with CAC-717 (26.67% ± 3.33%) versus distilled water (56.67% ± 8.82%). For non-contaminated seeds, there was no significant difference in germination rate and plant stem length between distilled water and CAC-717 treatment after 5 days of cultivation. In conclusion, CAC-717 is a promising seed disinfectant without deleterious effects on germination or plant growth.https://www.mdpi.com/2076-2607/8/10/1606bacteriumblack rotfood safetygerminationplant stem growthseeds |
| spellingShingle | Akikazu Sakudo Makoto Haritani Koichi Furusaki Rumiko Onishi Takashi Onodera Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage Seeds Microorganisms bacterium black rot food safety germination plant stem growth seeds |
| title | Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage Seeds |
| title_full | Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage Seeds |
| title_fullStr | Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage Seeds |
| title_full_unstemmed | Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage Seeds |
| title_short | Electrically Charged Disinfectant Containing Calcium Hydrogen Carbonate Mesoscopic Crystals as a Potential Measure to Control <i>Xanthomonas campestris</i> pv. <i>campestris</i> on Cabbage Seeds |
| title_sort | electrically charged disinfectant containing calcium hydrogen carbonate mesoscopic crystals as a potential measure to control i xanthomonas campestris i pv i campestris i on cabbage seeds |
| topic | bacterium black rot food safety germination plant stem growth seeds |
| url | https://www.mdpi.com/2076-2607/8/10/1606 |
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