Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis
Background: Multiple sclerosis (MS) is characterized by a combination of inflammatory and demyelination processes in the spinal cord and brain. Conventional drugs generally target the autoimmune response, without any curative effect. For that reason, there is a great interest in identifying novel ag...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8658 |
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| author | Ines ELBini-Dhouib Maroua Manai Nour-elhouda Neili Soumaya Marzouki Ghada Sahraoui Warda Ben Achour Sondes Zouaghi Melika BenAhmed Raoudha Doghri Najet Srairi-Abid |
| author_facet | Ines ELBini-Dhouib Maroua Manai Nour-elhouda Neili Soumaya Marzouki Ghada Sahraoui Warda Ben Achour Sondes Zouaghi Melika BenAhmed Raoudha Doghri Najet Srairi-Abid |
| author_sort | Ines ELBini-Dhouib |
| collection | DOAJ |
| description | Background: Multiple sclerosis (MS) is characterized by a combination of inflammatory and demyelination processes in the spinal cord and brain. Conventional drugs generally target the autoimmune response, without any curative effect. For that reason, there is a great interest in identifying novel agents with anti-inflammatory and myelinating effects, to counter the inflammation and cell death distinctive of the disease. Methods and results: An in vitro assay showed that curcumin (Cur) at 10 µM enhanced the proliferation of C8-D1A cells and modulated the production of Th1/Th2/Th17 cytokines in the cells stimulated by LPS. Furthermore, two in vivo pathophysiological experimental models were used to assess the effect of curcumin (100 mg/kg). The cuprizone model mimics the de/re-myelination aspect in MS, and the experimental autoimmune encephalomyelitis model (EAE) reflects immune-mediated events. We found that Cur alleviated the neurological symptomatology in EAE and modulated the expression of lymphocytes CD3 and CD4 in the spinal cord. Interestingly, Cur restored motor and behavioral deficiencies, as well as myelination, in demyelinated mice, as indicated by the higher index of luxol fast blue (LFB) and the myelin basic protein (MBP) intensity in the corpus callosum. Conclusions: Curcumin is a potential therapeutic agent that can diminish the MS neuroimmune imbalance and demyelination through its anti-inflammatory and antioxidant effects. |
| first_indexed | 2024-03-09T12:31:06Z |
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| id | doaj.art-ce8a237790474d6ebd33e538c0b3d015 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:31:06Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ce8a237790474d6ebd33e538c0b3d0152023-11-30T22:30:03ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315865810.3390/ijms23158658Dual Mechanism of Action of Curcumin in Experimental Models of Multiple SclerosisInes ELBini-Dhouib0Maroua Manai1Nour-elhouda Neili2Soumaya Marzouki3Ghada Sahraoui4Warda Ben Achour5Sondes Zouaghi6Melika BenAhmed7Raoudha Doghri8Najet Srairi-Abid9Laboratoire des Biomolécules, Venins et Applications Théranostiques (LR20IPT01), Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis 1002, TunisiaLaboratoire de Génétique Humaine (LR99ES10), Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis 2092, TunisiaLaboratoire des Biomolécules, Venins et Applications Théranostiques (LR20IPT01), Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis 1002, TunisiaLaboratoire de Transmission, Contrôle et Immunobiologie des Infections (LR11IPT02), Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis 1002, TunisiaLaboratoire de Médecine de Précision, Médecine Personnalisée et Investigation en Oncologie (LR21SP01), Service d’Anatomie Pathologique, Institut Salah Azaiez, Bab Saadoun, Tunis 1006, TunisiaLaboratoire des Biomolécules, Venins et Applications Théranostiques (LR20IPT01), Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis 1002, TunisiaLaboratoire des Biomolécules, Venins et Applications Théranostiques (LR20IPT01), Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis 1002, TunisiaLaboratoire de Transmission, Contrôle et Immunobiologie des Infections (LR11IPT02), Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis 1002, TunisiaLaboratoire de Médecine de Précision, Médecine Personnalisée et Investigation en Oncologie (LR21SP01), Service d’Anatomie Pathologique, Institut Salah Azaiez, Bab Saadoun, Tunis 1006, TunisiaLaboratoire des Biomolécules, Venins et Applications Théranostiques (LR20IPT01), Institut Pasteur de Tunis, Université de Tunis El Manar, Tunis 1002, TunisiaBackground: Multiple sclerosis (MS) is characterized by a combination of inflammatory and demyelination processes in the spinal cord and brain. Conventional drugs generally target the autoimmune response, without any curative effect. For that reason, there is a great interest in identifying novel agents with anti-inflammatory and myelinating effects, to counter the inflammation and cell death distinctive of the disease. Methods and results: An in vitro assay showed that curcumin (Cur) at 10 µM enhanced the proliferation of C8-D1A cells and modulated the production of Th1/Th2/Th17 cytokines in the cells stimulated by LPS. Furthermore, two in vivo pathophysiological experimental models were used to assess the effect of curcumin (100 mg/kg). The cuprizone model mimics the de/re-myelination aspect in MS, and the experimental autoimmune encephalomyelitis model (EAE) reflects immune-mediated events. We found that Cur alleviated the neurological symptomatology in EAE and modulated the expression of lymphocytes CD3 and CD4 in the spinal cord. Interestingly, Cur restored motor and behavioral deficiencies, as well as myelination, in demyelinated mice, as indicated by the higher index of luxol fast blue (LFB) and the myelin basic protein (MBP) intensity in the corpus callosum. Conclusions: Curcumin is a potential therapeutic agent that can diminish the MS neuroimmune imbalance and demyelination through its anti-inflammatory and antioxidant effects.https://www.mdpi.com/1422-0067/23/15/8658neuroinflammationdemyelinationcurcumin |
| spellingShingle | Ines ELBini-Dhouib Maroua Manai Nour-elhouda Neili Soumaya Marzouki Ghada Sahraoui Warda Ben Achour Sondes Zouaghi Melika BenAhmed Raoudha Doghri Najet Srairi-Abid Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis International Journal of Molecular Sciences neuroinflammation demyelination curcumin |
| title | Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis |
| title_full | Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis |
| title_fullStr | Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis |
| title_full_unstemmed | Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis |
| title_short | Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis |
| title_sort | dual mechanism of action of curcumin in experimental models of multiple sclerosis |
| topic | neuroinflammation demyelination curcumin |
| url | https://www.mdpi.com/1422-0067/23/15/8658 |
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