Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells
Imaging non-repetitive loci in living cells remains challenging. Here, the authors engineered an inducible system whereby biomolecular assemblies can be guided to specific genomic loci by a nuclease-defective Cas9, allowing the simultaneous imaging and manipulation of the loci.
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-35504-x |
| _version_ | 1797977365929787392 |
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| author | Qin Peng Ziliang Huang Kun Sun Yahan Liu Chi Woo Yoon Reed E. S. Harrison Danielle L. Schmitt Linshan Zhu Yiqian Wu Ipek Tasan Huimin Zhao Jin Zhang Sheng Zhong Shu Chien Yingxiao Wang |
| author_facet | Qin Peng Ziliang Huang Kun Sun Yahan Liu Chi Woo Yoon Reed E. S. Harrison Danielle L. Schmitt Linshan Zhu Yiqian Wu Ipek Tasan Huimin Zhao Jin Zhang Sheng Zhong Shu Chien Yingxiao Wang |
| author_sort | Qin Peng |
| collection | DOAJ |
| description | Imaging non-repetitive loci in living cells remains challenging. Here, the authors engineered an inducible system whereby biomolecular assemblies can be guided to specific genomic loci by a nuclease-defective Cas9, allowing the simultaneous imaging and manipulation of the loci. |
| first_indexed | 2024-04-11T05:05:41Z |
| format | Article |
| id | doaj.art-ce8a2c245cbe4bf2851e171ec04346fc |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-11T05:05:41Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-ce8a2c245cbe4bf2851e171ec04346fc2022-12-25T12:22:21ZengNature PortfolioNature Communications2041-17232022-12-0113111510.1038/s41467-022-35504-xEngineering inducible biomolecular assemblies for genome imaging and manipulation in living cellsQin Peng0Ziliang Huang1Kun Sun2Yahan Liu3Chi Woo Yoon4Reed E. S. Harrison5Danielle L. Schmitt6Linshan Zhu7Yiqian Wu8Ipek Tasan9Huimin Zhao10Jin Zhang11Sheng Zhong12Shu Chien13Yingxiao Wang14Department of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaInstitute of Cancer Research, Shenzhen Bay LaboratoryDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Pharmacology, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Biochemistry, University of Illinois at Urbana-ChampaignDepartment of Biochemistry, University of Illinois at Urbana-ChampaignDepartment of Pharmacology, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaDepartment of Bioengineering, Institute of Engineering in Medicine, University of CaliforniaImaging non-repetitive loci in living cells remains challenging. Here, the authors engineered an inducible system whereby biomolecular assemblies can be guided to specific genomic loci by a nuclease-defective Cas9, allowing the simultaneous imaging and manipulation of the loci.https://doi.org/10.1038/s41467-022-35504-x |
| spellingShingle | Qin Peng Ziliang Huang Kun Sun Yahan Liu Chi Woo Yoon Reed E. S. Harrison Danielle L. Schmitt Linshan Zhu Yiqian Wu Ipek Tasan Huimin Zhao Jin Zhang Sheng Zhong Shu Chien Yingxiao Wang Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells Nature Communications |
| title | Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells |
| title_full | Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells |
| title_fullStr | Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells |
| title_full_unstemmed | Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells |
| title_short | Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells |
| title_sort | engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells |
| url | https://doi.org/10.1038/s41467-022-35504-x |
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