Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis
We examined a cohort of 93 cystic fibrosis (CF) male patients who were pancreatic-sufficient (PS-CF; n=40) or pancreatic-insufficient (PI-CF; n = 53). Complex semen examination was performed, including standard semen analysis, quantitative karyological analysis (QKA) of immature germ cells (IGCs), t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2023;volume=25;issue=5;spage=591;epage=597;aulast=Sedova |
| _version_ | 1797649529597591552 |
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| author | Anna O Sedova Maria I Shtaut Elizaveta E Bragina Tatyana M Sorokina Galina V Shmarina Marina V Andreeva Lyubov F Kurilo Stanislav A Krasovskiy Aleksander V Polyakov Vyacheslav B Chernykh |
| author_facet | Anna O Sedova Maria I Shtaut Elizaveta E Bragina Tatyana M Sorokina Galina V Shmarina Marina V Andreeva Lyubov F Kurilo Stanislav A Krasovskiy Aleksander V Polyakov Vyacheslav B Chernykh |
| author_sort | Anna O Sedova |
| collection | DOAJ |
| description | We examined a cohort of 93 cystic fibrosis (CF) male patients who were pancreatic-sufficient (PS-CF; n=40) or pancreatic-insufficient (PI-CF; n = 53). Complex semen examination was performed, including standard semen analysis, quantitative karyological analysis (QKA) of immature germ cells (IGCs), transmission electronic microscopy (TEM), biochemical analysis, and sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL) assay. Azoospermia was diagnosed in 83 (89.2%) patients. The other 10 (10.8%) patients were found to be nonazoospermic and showed various spermatological diagnoses (asthenozoospermia, n = 2; asthenoteratozoospermia, n = 3; oligoasthenozoospermia, n = 1; oligoasthenoteratozoospermia, n = 3; and normozoospermia, n = 1) with no specific morphological abnormalities. Oligospermia was detected in 89.2% azoospermic and 30.0% nonazoospermic patients. Low seminal pH (<7.0) was found in 74 (89.2%) of 83 azoospermic patients. Moderate leukocytospermia (2.0 × 10[6]–2.2 × 10[6] ml−1) was revealed in 2.4% azoospermic and 40.0% nonazoospermic semen samples. The signs of partial meiotic arrest at prophase I were found in 4 of 6 nonazoospermic patients examined by QKA of IGCs. The content of fructose and citrate was low in oligospermic and normal in nonoligospermic semen samples. An increased percentage (>30%) of spermatozoa with noncondensed (“immature”) chromatin was revealed in 2 of 6 nonazoospermic semen samples analyzed by TEM. |
| first_indexed | 2024-03-11T15:47:29Z |
| format | Article |
| id | doaj.art-ce8be57f3df641f39b1392336f6cd004 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-03-11T15:47:29Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-ce8be57f3df641f39b1392336f6cd0042023-10-26T05:48:56ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622023-01-0125559159710.4103/aja2022115Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosisAnna O SedovaMaria I ShtautElizaveta E BraginaTatyana M SorokinaGalina V ShmarinaMarina V AndreevaLyubov F KuriloStanislav A KrasovskiyAleksander V PolyakovVyacheslav B ChernykhWe examined a cohort of 93 cystic fibrosis (CF) male patients who were pancreatic-sufficient (PS-CF; n=40) or pancreatic-insufficient (PI-CF; n = 53). Complex semen examination was performed, including standard semen analysis, quantitative karyological analysis (QKA) of immature germ cells (IGCs), transmission electronic microscopy (TEM), biochemical analysis, and sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL) assay. Azoospermia was diagnosed in 83 (89.2%) patients. The other 10 (10.8%) patients were found to be nonazoospermic and showed various spermatological diagnoses (asthenozoospermia, n = 2; asthenoteratozoospermia, n = 3; oligoasthenozoospermia, n = 1; oligoasthenoteratozoospermia, n = 3; and normozoospermia, n = 1) with no specific morphological abnormalities. Oligospermia was detected in 89.2% azoospermic and 30.0% nonazoospermic patients. Low seminal pH (<7.0) was found in 74 (89.2%) of 83 azoospermic patients. Moderate leukocytospermia (2.0 × 10[6]–2.2 × 10[6] ml−1) was revealed in 2.4% azoospermic and 40.0% nonazoospermic semen samples. The signs of partial meiotic arrest at prophase I were found in 4 of 6 nonazoospermic patients examined by QKA of IGCs. The content of fructose and citrate was low in oligospermic and normal in nonoligospermic semen samples. An increased percentage (>30%) of spermatozoa with noncondensed (“immature”) chromatin was revealed in 2 of 6 nonazoospermic semen samples analyzed by TEM.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2023;volume=25;issue=5;spage=591;epage=597;aulast=Sedovacongenital bilateral agenesia/aplasia of vas deferens; cystic fibrosis; cystic fibrosis transmembrane conductance regulator gene; semen; spermatozoa |
| spellingShingle | Anna O Sedova Maria I Shtaut Elizaveta E Bragina Tatyana M Sorokina Galina V Shmarina Marina V Andreeva Lyubov F Kurilo Stanislav A Krasovskiy Aleksander V Polyakov Vyacheslav B Chernykh Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis Asian Journal of Andrology congenital bilateral agenesia/aplasia of vas deferens; cystic fibrosis; cystic fibrosis transmembrane conductance regulator gene; semen; spermatozoa |
| title | Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis |
| title_full | Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis |
| title_fullStr | Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis |
| title_full_unstemmed | Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis |
| title_short | Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis |
| title_sort | comprehensive semen examination in patients with pancreatic sufficient and pancreatic insufficient cystic fibrosis |
| topic | congenital bilateral agenesia/aplasia of vas deferens; cystic fibrosis; cystic fibrosis transmembrane conductance regulator gene; semen; spermatozoa |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2023;volume=25;issue=5;spage=591;epage=597;aulast=Sedova |
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