Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae)
In this study, the complete mitogenome sequence of Rana amurensis (Anura: Ranidae) is determined using long PCR. It is a circular molecule of 20,571 bp in length (GenBank accession no. MF370348). The complete mtDNA sequence of R. amurensis contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA gene...
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Format: | Article |
Language: | English |
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Taylor & Francis Group
2017-12-01
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Series: | Mitochondrial DNA. Part B. Resources |
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Online Access: | http://dx.doi.org/10.1080/23802359.2017.1357444 |
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author | Peng Liu Heng Wang Wenge Zhao |
author_facet | Peng Liu Heng Wang Wenge Zhao |
author_sort | Peng Liu |
collection | DOAJ |
description | In this study, the complete mitogenome sequence of Rana amurensis (Anura: Ranidae) is determined using long PCR. It is a circular molecule of 20,571 bp in length (GenBank accession no. MF370348). The complete mtDNA sequence of R. amurensis contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs) and 2 control regions (D-loops). The nucleotide composition was 28.6% A, 26.8% C, 14.0% G and 30.6% T. Mitochondrial genome analyses based on NJ method yield phylogenetic trees, including that 20 reported family Ranidae frogs. These molecular data presented here provide a useful tool for systematic analyses of genus Rana. |
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format | Article |
id | doaj.art-ce8c5f26b7b44636a3b2179ca3db3160 |
institution | Directory Open Access Journal |
issn | 2380-2359 |
language | English |
last_indexed | 2024-03-11T13:36:44Z |
publishDate | 2017-12-01 |
publisher | Taylor & Francis Group |
record_format | Article |
series | Mitochondrial DNA. Part B. Resources |
spelling | doaj.art-ce8c5f26b7b44636a3b2179ca3db31602023-11-02T15:42:56ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592017-12-012242442510.1080/23802359.2017.13574441357444Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae)Peng Liu0Heng Wang1Wenge Zhao2Harbin Normal UniversityHarbin Normal UniversityHarbin Normal UniversityIn this study, the complete mitogenome sequence of Rana amurensis (Anura: Ranidae) is determined using long PCR. It is a circular molecule of 20,571 bp in length (GenBank accession no. MF370348). The complete mtDNA sequence of R. amurensis contained 2 rRNA genes (12S rRNA and 16S rRNA), 22 tRNA genes, 13 protein-coding genes (PCGs) and 2 control regions (D-loops). The nucleotide composition was 28.6% A, 26.8% C, 14.0% G and 30.6% T. Mitochondrial genome analyses based on NJ method yield phylogenetic trees, including that 20 reported family Ranidae frogs. These molecular data presented here provide a useful tool for systematic analyses of genus Rana.http://dx.doi.org/10.1080/23802359.2017.1357444ranidaemitogenomerana amurensisphylogenetic tree |
spellingShingle | Peng Liu Heng Wang Wenge Zhao Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae) Mitochondrial DNA. Part B. Resources ranidae mitogenome rana amurensis phylogenetic tree |
title | Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae) |
title_full | Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae) |
title_fullStr | Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae) |
title_full_unstemmed | Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae) |
title_short | Sequencing and analysis of the complete mitochondrial genome of Rana amurensis (Anura: Ranidae) |
title_sort | sequencing and analysis of the complete mitochondrial genome of rana amurensis anura ranidae |
topic | ranidae mitogenome rana amurensis phylogenetic tree |
url | http://dx.doi.org/10.1080/23802359.2017.1357444 |
work_keys_str_mv | AT pengliu sequencingandanalysisofthecompletemitochondrialgenomeofranaamurensisanuraranidae AT hengwang sequencingandanalysisofthecompletemitochondrialgenomeofranaamurensisanuraranidae AT wengezhao sequencingandanalysisofthecompletemitochondrialgenomeofranaamurensisanuraranidae |